Introduction

Pediatric heart failure imposes a significant health burden, necessitating effective interventions. Left ventricular assist devices (VADs) have emerged as crucial tools for circulatory support in advanced pediatric heart failure cases. However, VAD implantation brings forth the challenge of infections and inflammation, impacting patient outcomes. In this study, we explore the potential of two types pf pharmaceutical formulations, liposomal carriers loaded with gallic acid (GA) and tannic acid (TA) to address these issues.

Methods

Liposomes encapsulating GA and TA were prepared using thin-film hydration. Antimicrobial and antibiofilm efficacy against a dual bacterial system composed of Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis) was assessed. The impact on lipopolysaccharide (LPS)-induced human aortic endothelial cells (HAEC) viability, intercellular adhesion molecule 1(ICAM-1) expression, monocyte attachment, and Interleukin 6 (IL-6) production were analyzed.

Results

Both TA- and GA-loaded liposomes demonstrated uniform shape with size around 250 nm. TA-loaded liposomes exhibited superior antibacterial and antibiofilm efficacy against the dual bacteria system compared to GA-loaded liposomes. GA-loaded liposomes significantly improved HAEC viability but TA-liposomes did not substantially enhance cell viability. Both liposomal interventions reduced LPS-induced IL-6 production, ICAM-1 expression, and monocyte attachment on HAECs.

Conclusion

This study highlights the multifaceted potential of GA and TA-liposomes in addressing infections and inflammation associated with pediatric VAD implantation.

中文翻译：

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探索没食子酸和单宁酸输送的脂质体系统：解决儿科心室辅助装置接受者炎症和感染的潜在策略

介绍

小儿心力衰竭造成严重的健康负担，需要有效的干预措施。左心室辅助装置（VAD）已成为晚期儿科心力衰竭病例中循环支持的重要工具。然而，VAD 植入带来了感染和炎症的挑战，影响了患者的治疗结果。在这项研究中，我们探索了两种药物制剂的潜力，即装载没食子酸（GA）和单宁酸（TA）的脂质体载体来解决这些问题。

方法

采用薄膜水合法制备封装 GA 和 TA 的脂质体。评估了针对金黄色葡萄球菌（S. aureus）和表皮葡萄球菌（S. epidermidis ）组成的双细菌系统的抗菌和抗生物膜功效。分析了对脂多糖 (LPS) 诱导的人主动脉内皮细胞 (HAEC) 活力、细胞间粘附分子 1 (ICAM-1) 表达、单核细胞附着和白细胞介素 6 (IL-6) 产生的影响。

结果

负载 TA 和 GA 的脂质体均表现出均匀的形状，尺寸约为 250 nm。与负载 GA 的脂质体相比，负载 TA 的脂质体对双细菌系统表现出优异的抗菌和抗生物膜功效。负载 GA 的脂质体显着提高了 HAEC 的活力，但 TA 脂质体并没有显着增强细胞的活力。两种脂质体干预措施均减少了 LPS 诱导的 IL-6 产生、ICAM-1 表达和单核细胞在 HAEC 上的附着。

结论

这项研究强调了 GA 和 TA 脂质体在解决与儿科 VAD 植入相关的感染和炎症方面的多方面潜力。