Abstract—Plant polyphenols are characterized by a wide range of biological activities, including antioxidant properties, and have a high geroprotective potential. The purpose of this work was to investigate the effect of the extract of rowan berries (Sorbus aucuparia L.) on the lifespan and stress resistance of Drosophila melanogaster with the identification of possible mechanisms of its biological activity. It has been established that an ethanol extract of S. aucuparia berries, the main components of which are rutin and cyanidin-3-rutinoside, has a pronounced antioxidant activity in vitro. At the same time, treatment with rowan berry extract increased the resistance of D. melanogaster males to starvation, but reduced resistance to hyperthermia. In females, the extract reduced resistance to oxidative stress but increased resistance to hyperthermia. The effects of rowan berry extract on longevity depended both on its concentration and on the sex of fruit flies. In response to treatment with rowan berry extract, D. melanogaster males and females showed slight differences in the background level of expression of cellular stress response genes, including heat shock genes (hsp27, hsp68, and hsp83), oxidative stress resistance genes (hif1, nrf2, and sod1), circadian rhythm genes (clk and per), and the longevity gene sirt1, which may explain the differences in the observed effects.

摘要：植物多酚具有广泛的生物活性，包括抗氧化特性，并具有很高的防衰老潜力。这项工作的目的是研究罗文浆果（Sorbus aucuparia L.）提取物对果蝇寿命和抗逆性的影响，并确定其生物活性的可能机制。已证实，S. aucuparia浆果的乙醇提取物（其主要成分是芦丁和花青素-3-芸香苷）具有显着的体外抗氧化活性。同时，用罗文浆果提取物处理增加了雄性黑腹果蝇对饥饿的抵抗力，但降低了对高温的抵抗力。在女性中，提取物降低了对氧化应激的抵抗力，但增加了对高温的抵抗力。罗文浆果提取物对寿命的影响取决于其浓度和果蝇的性别。响应花楸浆果提取物的处理，雄性黑腹果蝇和雌性黑腹果蝇细胞应激反应基因的背景表达水平略有差异，包括热休克基因（hsp27、hsp68和hsp83）、氧化应激抗性基因（hif1、nrf2和sod1）、昼夜节律基因（clk和per）以及长寿基因Sirt1，这可以解释观察到的效果的差异。