Parabens are derivatives of alkyl esters of p-hydroxybenzoic acid and come in different classes. These compounds are primarily used as antimicrobial preservative agents in many commercial products, including cosmetics and pharmaceuticals. Accordingly, Benzyl paraben (BeP) is known to be a potential endocrine disruptor. The aim of this study was to determine the toxicity of benzyl paraben (BeP) on aquatic and terrestrial organisms, specifically Scenedesmus sp., Moina macrocopa, and Eisenia fetida. All the organisms were treated with different concentrations of BeP (0.025 mg/L and 1000 mg/L), and LC₂₅, LC₅₀, and LC₉₀ values were used to measure the toxicity levels. Results showed the LC values of BeP for M. macrocopa (3.3 mg/L, 4.7 mg/L, 7.3 mg/L) and E. fetida (173.2 mg/L, 479.8 mg/L, 1062 mg/L), respectively. Toxicity tests on green algae (Scenedesmus sp.) were conducted, the green algae were subjected to various BeP concentration. At 50 mg/L of BeP, cell viability was reduced to 56.2% and the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay indicated 35.4% viable cells. The chlorophyll value and the biochemical parameters of the algal cells were corroborative with the cell viability test. Lethal indices (LC₅₀) for M. macrocopa and E. fetida were evaluated for their toxicity on biochemical properties and were found to be catalase (0.111 mg/L, 0.5 mg/L), lipid peroxidation (0.072 mg/L, 0.056 mg/L), and total protein (0.309 mg/L, 0.314 mg/L), respectively. Overall, this study demonstrated the toxic impact of BeP on non-target aquatic as well as terrestrial species.

对羟基苯甲酸酯是对羟基苯甲酸烷基酯的衍生物，分为不同的类别。这些化合物主要用作许多商业产品中的抗菌防腐剂，包括化妆品和药品。因此，对羟基苯甲酸苄酯 (BeP) 被认为是一种潜在的内分泌干扰物。本研究的目的是确定对羟基苯甲酸苄酯 (BeP) 对水生和陆生生物的毒性，特别是栅藻、Moina macrocopa和Eisenia fetida。所有生物体均用不同浓度的BeP（0.025 mg/L和1000 mg/L）处理，并使用LC ₂₅、LC ₅₀和LC _{90值来测量毒性水平。}结果显示，BeP 对M. macrocopa的 LC 值分别为(3.3 mg/L、4.7 mg/L、7.3 mg/L) 和E. fetida (173.2 mg/L、479.8 mg/L、1062 mg/L)。对绿藻（Scenedesmus sp .）进行了毒性试验，对绿藻进行了不同BeP浓度的处理。在 50 mg/L BeP 下，细胞活力降低至 56.2%，MTT（3-[4,5-二甲基噻唑-2-基]-2,5 二苯基四唑溴化物）测定表明存活细胞为 35.4%。藻细胞的叶绿素值和生化参数与细胞活力测试相佐证。对M. macrocopa和E. fetida的致死指数(LC ₅₀ )进行生化毒性毒性评估，结果发现为过氧化氢酶(0.111 mg/L, 0.5 mg/L)、脂质过氧化(0.072 mg/L, 0.056 mg)。 /L）和总蛋白（0.309 mg/L，0.314 mg/L）。总体而言，这项研究证明了 BeP 对非目标水生和陆生物种的毒性影响。