Abstract

Sauger (Sander canadensis) and walleye (Sander vitreus) are closely related North American fish species that are often managed by fishery agencies throughout their ranges. However, genotyping resources for sauger are presently limited to a small set of microsatellite loci. We evaluated whether primers in an existing walleye genotyping-in-thousands panel could amplify single nucleotide polymorphism loci (SNPs) in sauger. We identified 71 primer pairs that amplify 118 SNPs in both species. Allele frequency differences were large enough to confidently distinguish the species and identify hybrids. Additionally, we identified 41 loci with observed heterozygosity > 0.1 in sauger; these markers may be useful for simple population genetic analyses and parentage analysis when few contributors are present and for differentiating highly structured populations.

中文翻译：

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适用于 sauger (Sander canadensis) 和 walleye (Sander vitreus) 物种鉴定和群体遗传学的扩增子基因分型面板

摘要

Sauger (Sander canadensis) 和 walleye (Sander vitreus) 是密切相关的北美鱼类，通常由渔业机构在其分布范围内进行管理。然而，索格的基因分型资源目前仅限于一小部分微卫星位点。我们评估了现有白斑眼斑眼鱼基因分型面板中的引物是否可以扩增索格中的单核苷酸多态性位点 (SNP)。我们鉴定了 71 个引物对，可在两个物种中扩增 118 个 SNP。等位基因频率差异足够大，足以自信地区分物种并识别杂种。此外，我们还鉴定了 41 个具有观察到的杂合性的基因座。 0.1 沙格；当贡献者很少时，这些标记可能有助于简单的群体遗传分析和亲子分析，以及区分高度结构化的群体。