Capra hircus (goat) induced pluripotent stem cells (giPSCs) harbor enormous scientific value and contribute to cellular agriculture, animal cloning, etc. Conventional approaches to giPSC generation suffer from complexity and low preparation efficiency. In the present study, we introduced the episomal vectors carrying the human pluripotent genes in goat somatic cells to generate the giPSC-like colonies. Initially, a simple non-enzymatic method was used to isolate the goat dermal fibroblast cells and, further, a cell line was established. Later, goat fibroblast cells were transfected with commercially available episomal vectors carrying the human pluripotent genes and successfully generated the iPSC-like colonies which exhibited the expression of goat endogenous pluripotent genes and positive staining with alkaline phosphatase. Moreover, giPS-like cells formed embryoid bodies (EBs)-like aggregates and weekly expressed the marker genes of two germ layers. Reprogramming of goat fibroblast using episomal vectors carrying human pluripotent genes could lead to the development of an efficient and time- and cost-effective approach to giPSC generation.

山羊诱导的多能干细胞 (giPSC) 具有巨大的科学价值，有助于细胞农业、动物克隆等。传统的 giPSC 生成方法复杂且制备效率低。在本研究中，我们将携带人类多能基因的附加型载体引入山羊体细胞中，以产生 giPSC 样集落。最初，采用简单的非酶法分离山羊真皮成纤维细胞，并进一步建立细胞系。随后，用市售的携带人多能基因的附加型载体转染山羊成纤维细胞，并成功产生了表达山羊内源多能基因并用碱性磷酸酶呈阳性染色的类iPSC集落。此外，giPS样细胞形成类胚体（EB）样聚集体，并每周表达两个胚层的标记基因。使用携带人类多能基因的附加型载体对山羊成纤维细胞进行重编程可能会导致开发出一种高效、省时且经济的 giPSC 生成方法。