Tectona grandis Linn, commonly known as teak, is traditionally used to treat a range of diseases, including the common cold, headaches, bronchitis, scabies, diabetes, inflammation, and others. The present study was conducted with the purpose of isolating and identifying the active compounds in T. grandis leaf against a panel of Vibrio spp., which may induce vibriosis in shrimp, using bioassay-guided purification. The antimicrobial activity was assessed using the microdilution method, followed by the brine shrimp lethality assay to determine toxicity. Following an initial screening with a number of different solvents, it was established that the acetone extract was the most effective. The acetone extract was then exposed to silica gel chromatography followed by reversed-phase HPLC and further UHPLC-orbitrap-ion trap mass spectrometry to identify the active compounds. Three compounds called 1-hydroxy-2,6,8-trimethoxy-9,10-anthraquinone, deoxyanserinone B, and khatmiamycin were identified with substantial anti-microbial action against V. parahaemolyticus, V. alginolyticus, V. harveyi, V. anguillarum, and V. vulnificus. The IC₅₀ values of the three compounds viz. 1-hydroxy-2,6,8-trimethoxy-9,10-anthraquinone, deoxyanserinone B, and khatmiamycin varied between 2 and 28, 7 and 38, and 7 and 56 μg/mL, respectively, which are as good as the standard antibiotics such as amoxicillin and others. The in vivo toxicity test revealed that the compounds were non-toxic to shrimp. The results of the study suggest that T. grandis leaf can be used as a source of bioactive compounds to treat Vibrio species in shrimp farming.

Tectona grandis Linn，俗称柚木，传统上用于治疗一系列疾病，包括普通感冒、头痛、支气管炎、疥疮、糖尿病、炎症等。本研究的目的是使用生物测定引导纯化，分离和鉴定巨观叶中针对一组弧菌的活性化合物，弧菌可能会诱导虾弧菌病。使用微量稀释法评估抗菌活性，然后通过丰年虾致死率测定来确定毒性。经过对多种不同溶剂的初步筛选后，确定丙酮提取物是最有效的。然后将丙酮提取物进行硅胶色谱分析，然后进行反相 HPLC 分析，并进一步进行 UHPLC-轨道阱-离子阱质谱分析，以鉴定活性化合物。被称为 1-羟基-2,6,8-三甲氧基-9,10-蒽醌、脱氧鹅舍利酮 B 和卡米霉素的三种化合物被鉴定对副溶血弧菌、溶藻弧菌、哈维弧菌、鳗弧菌具有显着的抗菌作用。和创伤弧菌。三种化合物的IC _50值即。1-羟基-2,6,8-三甲氧基-9,10-蒽醌、脱氧安丝里酮 B 和卡米霉素分别在 2 和 28、7 和 38、7 和 56 μg/mL 之间变化，与标准品一样好抗生素如阿莫西林等。体内毒性试验表明该化合物对虾无毒。研究结果表明，巨观叶可用作生物活性化合物的来源，用于治疗养虾业中的弧菌。