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Plant and yeast consortium for efficient remediation of dyes and effluents: a biochemical and toxicological study
International Microbiology ( IF 3.1 ) Pub Date : 2024-01-04 , DOI: 10.1007/s10123-023-00464-9
Rahul R. Jadhav , Savita R. Tapase , Vishal V. Chandanshive , Anna D. Gophane , Jyoti P. Jadhav

Textile effluent carries a range of dyes that may be recalcitrant and resistant to biodegradation. A unique consortium of the Fimbristylis dichotoma and Saccharomyces cerevisiae is exploited for the biodegradation of an azo dye Rubine GFL and actual textile effluent. This consortium enhances the rate of biodegradation of Rubine GFL and actual textile effluent with an excellent rate of biodegradation of 92% for Rubine GFL and 68% for actual textile effluent when compared to the individual one within 96 h. Speedy decolorization of Rubine GFL and actual textile effluent was observed due to the induction of oxido-reductive enzymes of the FD-SC consortium. Along with the significant reduction in the values of COD, BOD, ADMI, TSS, and TDS with 70, 64, 65, 41, and 52%, respectively, in experimental sets treated with FD-SC consortium. The biodegradation of Rubine GFL was confirmed with UV-Vis spectroscopy at the preliminary level, and then, metabolites formed after degradation were detected and identified by FTIR, HPLC, and GC-MS techniques. Also, decolorization of the dye was observed in the sections of the root cortex of Fimbristylis dichotoma. The toxicity of dye and metabolites formed after degradation was assessed by seed germination and bacterial count assay, where increased germination % and bacterial count from 31×107CFUs to 92 × 107 CFUs reflect the nontoxic nature of metabolites. Furthermore, the nontoxic nature of metabolites was confirmed by fish toxicity on Cirrhinus mrigala showed normal structures of fish gills and liver in the groups treated with FD-SC consortium proving the better tactic for biodegradation of dyes and textile effluent.



中文翻译:

用于有效修复染料和废水的植物和酵母联合体:生化和毒理学研究

纺织废水中含有一系列可能难以降解且难以生物降解的染料。Fimbristylis dichotomaSaccharomyces cerevisiae的独特联合体被用于偶氮染料 Rubine GFL 和实际纺织废水的生物降解。该联盟提高了 Rubine GFL 和实际纺织废水的生物降解率,与单独的生物降解率相比,96 小时内 Rubine GFL 的生物降解率高达 92%,实际纺织废水的生物降解率为 68%。由于 FD-SC 联合体氧化还原酶的诱导,观察到 Rubine GFL 和实际纺织废水的快速脱色。在使用 FD-SC 联合体处理的实验组中,COD、BOD、ADMI、TSS 和 TDS 值分别显着降低 70%、64%、65%、41% 和 52%。通过紫外-可见光谱初步证实了 Rubine GFL 的生物降解性,然后通过 FTIR、HPLC 和 GC-MS 技术检测和鉴定了降解后形成的代谢物。另外,在Fimbristylis dichotoma的根皮层切片中观察到染料脱色。通过种子发芽和细菌计数测定来评估降解后形成的染料和代谢物的毒性,其中发芽%和细菌计数从31×10 7 CFU增加到92×10 7 CFU反映了代谢物的无毒性质。此外,代谢物的无毒性质得到了对鲮鱼的毒性证实,在用 FD-SC 联合体处理的组中,鱼鳃和肝脏结构正常,证明了染料和纺织废水生物降解的更好策略。

更新日期:2024-01-05
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