Three simple and affordable UV spectrophotometric methods have been proposed for the simultaneous determination of chlorthalidone and nebivolol in a synthetic mixture, as well as a combined dosage form. Method I use the simultaneous equation methodology and has a linearity range of 5–25 μg/mL for chlorthalidone at 233 nm and 5–90 μg/mL for nebivolol at 280 nm respectively. The linearity ranges for chlorthalidone at 228–238 nm and nebivolol at 275–285 nm were found to be 5–60 and 5–100 μg/mL respectively, using method II, the area under the curve method. The linearity range for method III, the first derivative method, is 10–35 μg/mL for chlorthalidone at 227 nm and 10–35 μg/mL for nebivolol at 275 nm. The two diagnostic plot residuals normal probability plot and residuals versus expected values plot are utilized for the verification of outcome data and found to be optimal for three methods. The method has been validated for accuracy, precision, recovery studies, linearity, specificity, and stability studies according to the International Council of Harmonisation guideline Q2R1. These developed methods have been utilized in routine analysis for the simultaneous determination of chlorthalidone and nebivolol without pre-extraction.

提出了三种简单且经济实惠的紫外分光光度法，用于同时测定合成混合物以及组合剂型中的氯噻酮和奈必洛尔。方法 I 使用联立方程方法，氯噻酮在 233 nm 处的线性范围为 5–25 μg/mL，奈必洛尔在 280 nm 处的线性范围为 5–90 μg/mL。使用方法 II（曲线下面积法）发现氯噻酮在 228–238 nm 处和奈必洛尔在 275–285 nm 处的线性范围分别为 5–60 和 5–100 μg/mL。方法 III（一阶导数方法）的线性范围对于氯噻酮在 227 nm 处为 10–35 μg/mL，对于奈比洛尔在 275 nm 处为 10–35 μg/mL。两个诊断图残差正态概率图和残差与预期值图用于验证结果数据，并发现对于三种方法来说是最佳的。该方法已根据国际协调委员会指南 Q2R1 进行了准确性、精密度、回收率研究、线性、特异性和稳定性研究的验证。这些开发的方法已用于常规分析，无需预提取即可同时测定氯噻酮和奈必洛尔。