Pseudomonas spp., such as P. fluorescens group, P. fragi, and P. putida, are the major psychrophilic spoilage bacteria in the food industry. Bacteriophages (phages) are a promising tool for controlling food-spoilage and food-poisoning bacteria; however, there are few reports on phages effective on food-spoilage bacteria such as Pseudomonas spp. In this study, 12 Pseudomonas phages were isolated from chicken and soil samples. Based on the host range and lytic activity at 30 °C and 4 °C and various combinations of phages, phages vB_PflP-PCS4 and vB_PflP-PCW2 were selected to prepare phage cocktails to control Pseudomonas spp. The phage cocktail consisting of vB_PflP-PCS4 and vB_PflP-PCW2 showed the strongest lytic activity and retarded regrowth of P. fluorescens and P. putida at 30 °C, 8 °C, and 4 °C at a multiplicity of infection of 100. Nucleotide sequence analysis of the genomic DNA indicated that vB_PflP-PCS4 and vB_PflP-PCW2 phages were lytic phages of the Podoviridae family and lacked tRNA, toxin, or virulence genes. A novel endolysin gene was found in the genomic DNA of phage vB_PflP-PCS4. The results of this study suggest that the phage cocktail consisting of vB_PflP-PCS4 and vB_PflP-PCW2 is a promising tool for the biocontrol of psychrophilic food-spoilage pseudomonads during cold storage and distribution.

假单胞菌属，例如荧光假单胞菌、脆弱假单胞菌和恶臭假单胞菌，是食品工业中主要的嗜冷腐败菌。噬菌体（噬菌体）是控制食物腐败和食物中毒细菌的有前途的工具；然而，关于噬菌体对食品腐败菌（如假单胞菌属）有效的报道很少。在这项研究中，从鸡和土壤样品中分离出12 种假单胞菌噬菌体。根据宿主范围和30℃和4℃下的裂解活性以及噬菌体的各种组合，选择噬菌体vB_PflP-PCS4和vB_PflP-PCW2来制备噬菌体混合物来控制假单胞菌属。由 vB_PflP-PCS4 和 vB_PflP-PCW2 组成的噬菌体混合物在 30 °C、8 °C 和 4 °C、感染复数为 100 时表现出最强的裂解活性并延迟荧光假单胞菌和恶臭假单胞菌的再生。基因组 DNA 序列分析表明，vB_PflP-PCS4 和 vB_PflP-PCW2 噬菌体是 Podoviridae 家族的裂解性噬菌体，缺乏 tRNA、毒素或毒力基因。在噬菌体 vB_PflP-PCS4 的基因组 DNA 中发现了一个新的细胞内溶素基因。本研究的结果表明，由 vB_PflP-PCS4 和 vB_PflP-PCW2 组成的噬菌体混合物是冷藏和配送过程中嗜冷食品腐败假单胞菌生物防治的有前途的工具。