Background

The growth and development of rice (Oryza sativa L.) are affected by multiple factors, such as ROS homeostasis and utilization of iron. Here, we demonstrate that OsUGE2, a gene encoding a UDP-glucose 4-epimerase, controls growth and development by regulating reactive oxygen species (ROS) and iron (Fe) level in rice. Knockout of this gene resulted in impaired growth, such as dwarf phenotype, weakened root growth and pale yellow leaves. Biochemical analysis showed that loss of function of OsUGE2 significantly altered the proportion and content of UDP-Glucose (UDP-Glc) and UDP-Galactose (UDP-Gal). Cellular observation indicates that the impaired growth may result from decreased cell length. More importantly, RNA-sequencing analysis showed that knockout of OsUGE2 significantly influenced the expression of genes related to oxidoreductase process and iron ion homeostasis. Consistently, the content of ROS and Fe are significantly decreased in OsUGE2 knockout mutant. Furthermore, knockout mutants of OsUGE2 are insensitive to both Fe deficiency and hydrogen peroxide (H₂O₂) treatment, which further confirmed that OsUGE2 control rice growth possibly through Fe and H₂O₂ signal. Collectively, these results reveal a new pathway that OsUGE2 could affect growth and development via influencing ROS homeostasis and Fe level in rice.

中文翻译：

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OsUGE2 通过影响水稻中的 ROS 稳态和铁水平来调节植物生长

背景

水稻( Oryza sativa  L.)的生长发育受到ROS稳态和铁利用等多种因素的影响。在这里，我们证明OsUGE2（一种编码 UDP-葡萄糖 4-差向异构酶的基因）通过调节水稻中的活性氧 (ROS) 和铁 (Fe) 水平来控制生长和发育。该基因的敲除会导致生长受损，例如矮化表型、根系生长减弱和叶片淡黄。生化分析表明， OsUGE2功能丧失显着改变了 UDP-葡萄糖 (UDP-Glc) 和 UDP-半乳糖 (UDP-Gal) 的比例和含量。细胞观察表明生长受损可能是由于细胞长度缩短造成的。更重要的是，RNA测序分析表明OsUGE2的敲除显着影响与氧化还原酶过程和铁离子稳态相关的基因的表达。一致地， OsUGE2敲除突变体中 ROS 和 Fe 的含量显着降低。此外，OsUGE2的敲除突变体对Fe缺乏和过氧化氢(H ₂ O ₂ )处理均不敏感，这进一步证实OsUGE2可能通过Fe和H ₂ O ₂信号控制水稻生长。总的来说，这些结果揭示了OsUGE2可以通过影响水稻中 ROS 稳态和 Fe 水平来影响生长和发育的新途径。