Abstract

This study describes the in vitro regeneration of ‘Haruka’ plants, a new cultivar of Japanese flowering cherry registered in 2021 by the Japanese statutory authority. As this is a double-flowered cultivar produced by inter-specific hybridization, in vitro regeneration is an effective method for large-scale propagation. To promote proliferation, apical shoots were cultured on half-strength Murashige and Skoog medium supplemented with 5 μM 6-benzylaminopurine. The highest average of 7.8 shoots per explant was obtained at 15°C in the dark. Cultures were maintained in continuous darkness for 12 wk and then transferred to lighting conditions under a 16-h photoperiod at 25°C. Subsequently, a 100% rooting rate was obtained with the application of 1 μM indole-3-butyric acid in combination with 0.1 μM naphthaleneacetic acid. Additionally, the number of roots per shoot and the maximum length of roots were significantly higher under exposure to pink light illumination provided by cold cathode fluorescent lamps emitting red- and blue-colored light at a ratio of 80% and 20%, respectively. More than 95% of the regenerated plantlets survived after ex vitro acclimatization.

中文翻译：

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日本樱花新品种“Haruka”（Cerasus Sato-zakura Group“Haruka”）的组织培养响应和体外植株再生

摘要

这项研究描述了“Haruka”植物的体外再生，“Haruka”是日本法定机构于 2021 年注册的日本樱花新品种。由于该品种是通过种间杂交产生的重花品种，离体再生是大规模繁殖的有效方法。为了促进增殖，在补充有 5 μM 6-苄氨基嘌呤的半强度 Murashige 和 Skoog 培养基上培养顶芽。每个外植体在 15°C 的黑暗条件下平均获得最高平均 7.8 个芽。将培养物在连续黑暗中维持12周，然后转移至25°C、16小时光周期的光照条件下。随后，使用 1 μM 吲哚-3-丁酸与 0.1 μM 萘乙酸组合，获得 100% 的生根率。此外，在冷阴极荧光灯分别以80%和20%的比例发射红光和蓝光提供的粉红光照射下，每个芽的根数和根的最大长度显着更高。体外驯化后，95%以上的再生植株成活。