Cytochrome P450s are a large family of protein-encoding genes in plant genomes, many of which have not yet been comprehensively characterized. Here, a novel P450 gene, CYP82D47, was isolated and functionally characterized from cucumber (Cucumis sativus L.). Quantitative real-time reverse-transcription polymerase chain reaction analysis revealed that CYP82D47 expression was triggered by salicylic acid (SA) and ethephon (ETH). Expression analysis revealed a correlation between CYP82D47 transcript levels and plant defense responses against powdery mildew (PM) and Fusarium oxysporum f. sp. cucumerinum (Foc). Although no significant differences were observed in disease resistance between CYP82D47-RNAi and wild-type cucumber, overexpression (OE) of CYP82D47 enhanced PM and Foc resistance in cucumber. Furthermore, the expression levels of SA-related genes (PR1, PR2, PR4, and PR5) increased in CYP82D47-overexpressing plants 7 days post fungal inoculation. The levels of ETH-related genes (EIN3 and EBF2) were similarly upregulated. The observed enhanced resistance was associated with the upregulation of SA/ETH-signaling-dependent defense genes. These findings indicate the crucial role of CYP82D47 in pathogen defense in cucumber. CYP82D47-overexpressing cucumber plants exhibited heightened susceptibility to both diseases. The study results offer important insights that could aid in the development of disease-resistant cucumber cultivars and elucidate the molecular mechanisms associated with the functions of CYP82D47.

细胞色素 P450 是植物基因组中蛋白质编码基因的一个大家族，其中许多基因尚未得到全面表征。在这里，从黄瓜 ( Cucumis sativus L.)中分离出一个新的 P450 基因CYP82D47并进行了功能表征。定量实时逆转录聚合酶链反应分析表明，CYP82D47 的表达是由水杨酸（SA）和乙烯利（ETH）触发的。表达分析揭示了CYP82D47转录物水平与植物针对白粉病 (PM) 和尖孢镰刀菌 ( Fusarium oxysporum f) 的防御反应之间的相关性。sp 。黄瓜（Foc）。虽然CYP82D47 -RNAi和野生型黄瓜之间的抗病性没有观察到显着差异，但CYP82D47的过表达（OE）增强了黄瓜的PM和Foc抗性。此外，在真菌接种后7天，CYP82D47过表达植物中SA相关基因（PR1、PR2、PR4和PR5）的表达水平增加。ETH 相关基因（ EIN3和EBF2 ）的水平也同样上调。观察到的抵抗力增强与 SA/ETH 信号依赖性防御基因的上调有关。这些发现表明CYP82D47在黄瓜病原体防御中的关键作用。CYP82D47过度表达的黄瓜植物对这两种疾病表现出更高的易感性。该研究结果提供了重要的见解，可以帮助开发抗病黄瓜品种，并阐明与CYP82D47功能相关的分子机制。