Abstract

Background

Platycladus orientalis (L.) Franco (PO) has been used in Oriental medicine to treat rheumatism and diarrhea; however, its antinociceptive effects remain unclear.

Objective

This study aimed to reveal the antinociceptive effect of the PO extract and standardize this extract in RAW 264.7 cells and mice with monosodium uric acid (MSU)-induced pain.

Results

PO extracts were prepared using different ethanol (EtOH) concentrations (0–100%). Thereafter, groups were determined based on the results of the MSU-induced pain model in mice and nitric oxide (NO) assay using lipopolysaccharide (LPS)-induced RAW 264.7 cells. As the methylene chloride (CH₂Cl₂) and ethyl acetate (EtOAc) layers exhibited increasing effects with time, these layers were selected to isolate the major components. The PO extract was standardized using a highly efficient HPLC method, and its antinociceptive effects in a mouse model of MSU-induced gout pain were determined using the von Frey test. The PO extract was found to exhibit antinociceptive effects. Moreover, sample standardization was successfully achieved based on the validation of these compounds.

Conclusion

The PO extract exhibited potent dose- and time-dependent antinociceptive effects in an animal model of gout pain; these effects might be mediated by the major compounds of this extract. Collectively, these results suggest that PO can be used as a herbal drug to treat gout-related pain.

中文翻译：

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侧柏提取物的镇痛作用评价及标准化

摘要

背景

Platycladus orientalis (L.) Franco (PO) 在东方医学中用于治疗风湿病和腹泻；然而，其抗伤害作用仍不清楚。

客观的

本研究旨在揭示 PO 提取物的抗伤害作用，并在 RAW 264.7 细胞和单钠尿酸 (MSU) 引起的疼痛小鼠中标准化该提取物。

结果

使用不同浓度的乙醇 (EtOH) (0–100%) 制备 PO 提取物。此后，根据 MSU 诱导的小鼠疼痛模型的结果和使用脂多糖 (LPS) 诱导的 RAW 264.7 细胞进行一氧化氮 (NO) 测定的结果来确定分组。由于二氯甲烷(CH ₂ Cl ₂ )和乙酸乙酯(EtOAc)层随着时间的推移表现出增强的效果，因此选择这些层来分离主要组分。使用高效 HPLC 方法对 PO 提取物进行标准化，并使用 von Frey 试验测定其在 MSU 诱发的痛风小鼠模型中的镇痛作用。PO 提取物被发现具有抗伤害作用。此外，基于这些化合物的验证，成功实现了样品标准化。

结论

PO 提取物在痛风动物模型中表现出有效的剂量和时间依赖性镇痛作用；这些作用可能是由该提取物的主要化合物介导的。总的来说，这些结果表明 PO 可用作治疗痛风相关疼痛的草药。