The distinct and species-specific chemical compounds found on the insect cuticle have demonstrated effectiveness in various applications, including species identification. Accurate identification of fly species becomes challenging when only damaged empty puparial cases are available, making it difficult to use traditional morphological and molecular identification methods. This study aimed to analyze the chemical compositions of puparial exuviae from three forensically and medically important fly species; Lucilia sericata, Chrysomya albiceps, and Chrysomya marginalis. Gas chromatography/mass spectrometry (GC–MS) was employed to assess the chemical profiles of these exuviae and evaluate their accuracy in identifying Dipteran insects. The study revealed the presence of twelve classes of chemical compounds across the three species, with retention times ranging from 18.78 to 35.03. A total of forty-two compounds with chain lengths ranging from C12 to C45 were identified. The profiles of Ch. albiceps and L. sericata displayed similarities, with alcohol being the most abundant compound (28.6%) in L. sericata. However, alkanes, including n-alkanes, branched alkanes, and cycloalkanes, constituted the main components of the cuticles in the three species, with Ch. marginalis displaying the highest percentage. These findings represent an initial step towards utilizing hydrocarbon composition as a practical tool for distinguishing between forensic species in Egypt.

在昆虫角质层上发现的独特且物种特异性的化合物已在各种应用中证明了有效性，包括物种鉴定。当只有受损的空蛹盒可用时，准确识别蝇种变得具有挑战性，使得很难使用传统的形态学和分子鉴定方法。本研究旨在分析三种法医学和医学上重要的蝇类蛹蜕皮的化学成分；丝光绿蝇、白头金蝇和边缘金蝇。采用气相色谱/质谱（GC-MS）评估这些蜕皮的化学特征，并评估其识别双翅目昆虫的准确性。该研究揭示了这三个物种中存在十二类化合物，保留时间范围为 18.78 至 35.03。总共鉴定了 42 种链长范围从 C12 到 C45 的化合物。 Ch的简介。白头肌和L. sericata显示出相似之处，酒精是L. sericata中最丰富的化合物 (28.6%) 。然而，烷烃，包括正烷烃、支链烷烃和环烷烃，构成了这三个物种角质层的主要成分，其中Ch.边缘显示百分比最高。这些发现代表了利用碳氢化合物成分作为区分埃及法医物种的实用工具的第一步。