Abstract

The red palm weevil (RPW) Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is a species of beetle that depends on palm trees to complete its life cycle. RPW larvae feed on palm trunks and can cause tree death, with significant impacts on both wild and cultivated palms. The larvae burrow into the trunk of the palm and feed on the delicate tissue and sap of the palm, killing the host tree. Herbivore insect gut microbiota play important roles in host physiological characteristics such as nutritional needs, immunity, growth and developmental mechanisms, and mating behavior. The aim of this study was to determine the gut microbiota diversity of RPW larvae sampled in Sistan and Baluchistan province, Iran. Analysis of 16S rRNA amplified directly from the intestine showed the presence of Serratia sp., Klebsiella sp., and Shigella sp. from the Enterobacteriaceae family, Bacillus sp. from the Bacillacea family, Enterococcus sp. from the Enterococcaceae family, and Kocuria sp. from the Micrococcaceae family. The results of their cultivation on a modified CMCAgar medium showed that Kocuria, Serratia, Bacillus, Shigella genera have the ability to degrade cellulose. Identifying the bacterial flora and determining their role can be essential to controlling and reducing the population of this pest.

中文翻译：

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铁红喙头蝠（鞘翅目：象甲科）幼虫肠道菌群多样性调查

摘要

红棕榈象甲 (RPW) Rhynchophorus ferrugineus（鞘翅目：象甲科）是一种依赖棕榈树完成其生命周期的甲虫。 RPW 幼虫以棕榈树干为食，可导致树木死亡，对野生和栽培棕榈树都有重大影响。幼虫钻入棕榈树的树干中，以棕榈树脆弱的组织和汁液为食，杀死寄主树。草食动物昆虫肠道微生物群在宿主生理特征（如营养需求、免疫、生长和发育机制以及交配行为）中发挥着重要作用。本研究的目的是确定伊朗锡斯坦和俾路支省采样的 RPW 幼虫的肠道微生物群多样性。对直接从肠道扩增的 16S rRNA 进行分析表明存在沙雷氏菌属、克雷伯氏菌属和志贺氏菌属。来自肠杆菌科，芽孢杆菌属。来自杆菌科，肠球菌属。来自肠球菌科和Kocuria sp。来自微球菌科。在改良的CMCAgar培养基上培养的结果表明，库库菌属、沙雷氏菌属、芽孢杆菌属、志贺氏菌属具有降解纤维素的能力。识别细菌菌群并确定它们的作用对于控制和减少这种害虫的数量至关重要。