Abstract

Heading date is one of the important agronomic traits that affects rice yield. In this study, we cloned a new rice B3 family gene, OsL1, which regulates rice heading date. Importantly, osl1-1 and osl1-2, two different types of mutants of OsL1 were created using the gene editing technology CRISPR/Cas9 system and exhibited 4 days earlier heading date than that of the wild type under short-day conditions. Subsequently, the plants overexpressing OsL1, OE-OsL1, showed a 2-day later heading date than the wild type in Changsha and a 5-day later heading date in Lingshui, but there was no significant difference in other yield traits. Moreover, the results of subcellular localization study indicated that OsL1 protein was located in the nucleus and the expression pattern analysis showed that OsL1 gene was expressed in rice roots, stems, leaves, and panicles, and the expression level was higher at the root and weak green panicle. In addition, the OsL1 gene was mainly expressed at night time under short-light conditions. The transcriptomic analysis indicated that OsL1 might be involved in the Hd1-Hd3a pathway function. Together, our results revealed that the cloning and functional analysis of OsL1 can provide new strategy for molecular design breeding of rice with suitable fertility period.

中文翻译：

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OsL1基因功能丧失导致水稻在短日照条件下提早开花

摘要

抽穗期是影响水稻产量的重要农艺性状之一。在这项研究中，我们克隆了一个新的水稻 B3 家族基因OsL1，它调节水稻抽穗日期。重要的是，OsL1的两种不同类型的突变体osl1-1和osl1-2是利用基因编辑技术CRISPR/Cas9系统创建的，并且在短日照条件下表现出比野生型早4天的抽穗期。随后，过表达OsL1的植株OE-OsL1在长沙比野生型晚2天抽穗期，在陵水比野生型晚5天抽穗期，但其他产量性状没有显着差异。此外，亚细胞定位研究结果表明OsL1蛋白定位于细胞核，表达谱分析表明OsL1基因在水稻根、茎、叶、穗中均有表达，且根部表达量较高，而根部表达量较弱。绿色圆锥花序。此外，OsL1基因主要在夜间短光条件下表达。转录组分析表明OsL1可能参与Hd1-Hd3a通路功能。总之，我们的结果表明， OsL1的克隆和功能分析可以为适育期水稻的分子设计育种提供新策略。