Background

Bergenin is a type of polyphenol derived from various medicinal plants and has multiple biological functions, including antioxidant, anti-cancerous, and anti-inflammatory activity. However, the role of bergenin in atherosclerosis (AS) development has not been detected yet. Here, human umbilical vein endothelial cells (HUVECs) were used to investigate the effects of bergenin on TNF-α-induced oxidative stress, inflammation, and monocyte adhesion in vitro.

Methods

Cell viability of HUVECs was assessed by cell counting kit-8 (CCK-8) assay. Western blotting was performed to evaluate the levels of apoptosis- or signaling-related proteins. Intracellular oxidative stress levels were detected by evaluating reactive oxygen species (ROS) production, malondialdehyde (MDA) level, and superoxide dismutase (SOD) activity in HUVECs. The effects of bergenin on monocyte adhesion to HUVECs were detected by measuring the protein and expression levels of adhesion molecules.

Results

Bergenin promoted the viability and inhibited the apoptosis in TNF-α-treated HUVECs. The increased oxidative stress induced by TNF-α was significantly suppressed by bergenin in a concentration-dependent manner. Bergenin reduced the protein and expression levels of adhesion molecules in TNF-α-treated HUVECs. Human leukemic monocyte (U973) adhesion to HUVECs was promoted by TNF-α treatment and significantly inhibited by bergenin. In addition, bergenin blocked the activation of NF-κB signaling in TNF-α-treated HUVECs.

Conclusion

Bergenin inhibited TNF-α-induced apoptosis and oxidative stress in HUVECs and suppressed monocyte adhesion to HUVECs by inactivating NF-κB signaling pathway.

中文翻译：

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岩白菜素通过抑制人脐静脉内皮细胞的氧化应激、炎症和单核细胞粘附来改善动脉粥样硬化的进展

背景

岩白菜素是一种从多种药用植物中提取的多酚，具有多种生物功能，包括抗氧化、抗癌和抗炎活性。然而，岩白菜素在动脉粥样硬化（AS）发展中的作用尚未被发现。本研究使用人脐静脉内皮细胞 (HUVEC) 体外研究岩白菜素对 TNF-α 诱导的氧化应激、炎症和单核细胞粘附的影响。

方法

通过细胞计数试剂盒 8 (CCK-8) 测定评估 HUVEC 的细胞活力。进行蛋白质印迹以评估细胞凋亡或信号传导相关蛋白的水平。通过评估 HUVEC 中活性氧 (ROS) 的产生、丙二醛 (MDA) 水平和超氧化物歧化酶 (SOD) 活性来检测细胞内氧化应激水平。通过测定粘附分子的蛋白和表达水平来检测岩白菜素对单核细胞粘附HUVEC的影响。

结果

岩白菜素可促进 TNF-α 处理的 HUVEC 的活力并抑制细胞凋亡。TNF-α 诱导的氧化应激增加被岩白菜素以浓度依赖性方式显着抑制。岩白菜素降低了 TNF-α 处理的 HUVEC 中粘附分子的蛋白质和表达水平。TNF-α 处理可促进人白血病单核细胞 (U973) 与 HUVEC 的粘附，而岩白菜素可显着抑制该作用。此外，岩白菜素还可阻断 TNF-α 处理的 HUVEC 中 NF-κB 信号传导的激活。

结论

Bergenin 抑制 TNF-α 诱导的 HUVEC 细胞凋亡和氧化应激，并通过灭活 NF-κB 信号通路抑制单核细胞对 HUVEC 的粘附。