As a common malignant tumor, esophageal squamous cell carcinoma (ESCC) is occasionally seen in clinical practice. This type of disease has low incidence rate and mortality. The post-translational modification of small ubiquitin like modifiers (SUMO) can play a crucial role in regulating protein function, and can significantly impact the occurrence and development of diseases. SUMO-specific peptidase (SENP) affects cell activity by regulating the biological function of SUMO. SENP3 belongs to the SENP family, and available data indicate that many malignancies are associated with SENPs, it is currently unclear its role in ESCC. This study indicates that there is a high level of SENP3 expression in ESCC tumor cells. If the expression level of this gene is high, it can have a significant impact on ESCC cell lines and affect physiological activities such as invasion of KYSE170 cells. If the gene is knocked out, this situation will not occur. There is also research data indicating that this gene can effectively activate related signaling pathways, thereby promoting the physiological activities of malignant tumor cells. In a nude mouse xenograft tumor model, KYSE170 cells with SENP3 expression knockdown induced a smaller volume and weight of tumor tissue. Therefore, it can be clearly stated that SENP3 can enable Wnt/ β- The catenin signaling pathway is stimulated, which in turn affects the physiological activities of ESCC cells, including the invasion process. The results of this article lay the foundation for clinical staff to carry out clinical management.

食管鳞状细胞癌（ESCC）作为一种常见的恶性肿瘤，在临床上偶尔可见。此类疾病的发病率和死亡率较低。小泛素样修饰物（SUMO）的翻译后修饰在调节蛋白质功能中发挥着至关重要的作用，对疾病的发生和发展具有显着影响。SUMO 特异性肽酶 (SENP) 通过调节 SUMO 的生物学功能来影响细胞活性。SENP3属于SENP家族，现有数据表明许多恶性肿瘤与SENP相关，目前尚不清楚其在食管鳞癌中的作用。本研究表明食管鳞癌肿瘤细胞中有高水平的SENP3表达。如果该基因的表达水平较高，则可对ESCC细胞系产生显着影响，影响KYSE170细胞的侵袭等生理活动。如果基因被敲除，这种情况就不会发生。还有研究数据表明，该基因能够有效激活相关信号通路，从而促进恶性肿瘤细胞的生理活动。在裸鼠异种移植肿瘤模型中，敲低 SENP3 表达的 KYSE170 细胞诱导出更小的肿瘤组织体积和重量。因此，可以明确地说，SENP3能够使Wnt/β-catenin信号通路受到刺激，进而影响ESCC细胞的生理活动，包括侵袭过程。本文研究结果为临床人员开展临床管理奠定了基础。