Globally, millions of cubic meters of dye-contaminated wastewater are generated annually from the textile industry. Discharging the dye-contaminated wastewater into natural water streams is detrimental to the ecosystem and human health. Wastewater bioremediation is considered a practical solution for converting wastewater into value-added water for safe reuse especially in irrigation. The present study investigated the immobilization of highly stable spore laccase enzyme of Bacillus amyloliquefaciens A1 by entrapment and adsorption techniques. Based on the results, agarose gel was the most efficient support with 94% immobilization yield. Optimum temperature of immobilized enzyme was at 60 ℃ with complete thermal stability for 120 min. The maximum activity of immobilized enzyme was at pH 6–7 and retained 91% of its activity after 24 h incubation. It showed 100% storage stability after 8 weeks which was higher than that of un-immobilized enzyme (4 weeks). The enzyme activity was nearly stable after 8 consecutive cycles of reuse. Decolorization of 8 textile dyes was studied using the immobilized enzyme. Crystal violet, direct red 81 and acid black 24 were efficiently decolorized (94.5, 77.4, and 68.8%, respectively) within 24 h in the first cycle. Reusability of immobilized enzyme showed 74.6, 53.5, and 51.8% decolorization of the same dyes in the same order after the second cycle. Immobilized spore laccase-treated dye water was used to irrigate lentil seeds and showed germination index about 73% compared to 10.8% for untreated one indicating very low toxicity of the treated dye water.

全球范围内，纺织行业每年产生数百万立方米的染料污染废水。将染料污染的废水排放到天然水流中对生态系统和人类健康有害。废水生物修复被认为是将废水转化为增值水以安全再利用的实用解决方案，特别是在灌溉方面。本研究通过包埋和吸附技术研究了高度稳定的解淀粉芽孢杆菌A1孢子漆酶的固定化。根据结果​​，琼脂糖凝胶是最有效的支持物，固定化率为 94%。固定化酶的最适温度为60 ℃，热稳定120 min。固定化酶的最大活性在pH 6-7 时，培养24 h 后仍保留91% 的活性。8周后其储存稳定性为100%，高于非固定化酶（4周）。连续8次循环使用后酶活性基本稳定。利用固定化酶对8种纺织染料的脱色进行了研究。在第一个循环中，结晶紫、直接红 81 和酸性黑 24 在 24 小时内被有效脱色（分别为 94.5%、77.4% 和 68.8%）。固定化酶的可重复使用性显示，在第二次循环后，相同染料按相同顺序脱色率为 74.6%、53.5% 和 51.8%。使用固定化孢子漆酶处理的染料水来灌溉扁豆种子，其发芽指数约为 73%，而未经处理的发芽指数为 10.8%，表明处理过的染料水的毒性非常低。