In the field of forensic entomology, Chrysomya rufifacies (Macquart 1843) (Diptera: Calliphoridae) has proven to be an important species for postmortem interval (PMI) assessment in criminal investigations. The developmental patterns of C. rufifacies exhibit temperature-dependent variations that are geographically specific, and these variations are influenced by seasonal changes. Hence, understanding the geographically specific development patterns of C. rufifacies in response to temperature and seasonal changes is crucial for improving the accuracy of PMI predictions. In the present study, we examined the developmental rates of C. rufifacies across a temperature gradient (20 °C, 25 °C, 30 °C, 35 °C, and 40 °C). Primary objective of present study was to gather precise developmental data essential for calculating the post-mortem interval (PMI) within varying temperature ranges. Furthermore, we investigated the influence of seasonal variations, encompassing the summer, rainy and winter seasons, on the growth and developmental patterns of C. rufifacies. The results of present studies show that temperature significantly affects the growth and development of C. rufifacies. The length of developmental stages and morphological parameters varied seasonally, with longer duration and colder temperatures in winter and shorter duration and warmer temperatures in summer. The finding that faster development was directly correlated with higher temperatures highlighted the significance of temperature in affecting an insect’s life cycle. Our research highlights the necessity of gathering information on evolution trends that are specific to a given area in order to accurately calculate PMI in forensic entomological investigations. Understanding the interactions between temperature, seasonal variation, and morphological traits is critical for establishing baseline data for PMI estimates. The accuracy of PMI estimates will ultimately increase thanks to this information, and forensic entomologists will have even more opportunities to contribute to forensic investigations.

在法医昆虫学领域，Chrysomya rufifacies（Macquart 1843）（双翅目：Calliphoridae）已被证明是刑事调查中死后间隔（PMI）评估的重要物种。C. rufifacies的发育模式表现出地理上特定的温度依赖性变化，并且这些变化受到季节变化的影响。因此，了解C. rufifacies响应温度和季节变化的地理特定发育模式对于提高 PMI 预测的准确性至关重要。在本研究中，我们研究了C. rufifacies在温度梯度（20 °C、25 °C、30 °C、35 °C 和 40 °C）下的发育速率。本研究的主要目的是收集精确的发育数据，这些数据对于计算不同温度范围内的死后间隔（PMI）至关重要。此外，我们还研究了季节变化（包括夏季、雨季和冬季）对C. rufifacies生长和发育模式的影响。目前的研究结果表明，温度对C. rufifacies的生长发育有显着影响。发育阶段的长度和形态参数随季节变化，冬季持续时间较长、温度较低，夏季持续时间较短、温度较高。更快的发育与更高的温度直接相关这一发现凸显了温度在影响昆虫生命周期中的重要性。我们的研究强调了收集特定区域特定进化趋势信息的必要性，以便在法医昆虫学调查中准确计算 PMI。了解温度、季节变化和形态特征之间的相互作用对于建立 PMI 估计的基线数据至关重要。由于这些信息，PMI 估计的准确性最终将提高，法医昆虫学家将有更多机会为法医调查做出贡献。