The failure to successfully treat dermatophyte-related diseases is often due to the formation of biofilms, which makes dermatophytes resistant to antifungals. Here, an attempt has been made to assess inhibition of dermatophyte biofilm production using photodynamic therapy and rhamnolipid biosurfactant. Two methods were used to inhibit biofilm formation by dermophytes Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton verrucosum, Microsporum canis and Microsporum gypseum. The first method was the use of rhamnolipid with concentrations of 39 to 1000 ppm and the second was the use of photodynamic method with concentrations of 8, 16 and 32 µg/ml of methylene blue. In addition, these two methods were evaluated simultaneously. The biofilm formation was evaluated using spectrophotometry and scanning electron microscopy. Biosurfactant has been shown to have an improved ability to inhibit the formation of biofilm by the strains. Although photodynamic therapy has not been successful, but in combination with biosurfactant, it may have a synergistic effect. By investigating the effect of rhamnolipid on the formation of biofilm, it was found that Microsporum species has a relatively stronger attachment to the surfaces of the wells compared to trichophyton species. The biofilms were evaluated with electron microscope in the simultaneous treatment of rhamnolipid and photodynamics. The results showed that after the treatment, the biofilms became discrete and their structural integrity was reduced. Even in Microsporum species, which were among the most resistant dermatophytes, the changes in the fungal biofilm after treatment were significant.

未能成功治疗皮肤癣菌相关疾病通常是由于生物膜的形成，这使得皮肤癣菌对抗真菌药物产生抗药性。在这里，尝试评估使用光动力疗法和鼠李糖脂生物表面活性剂对皮肤癣菌生物膜产生的抑制。采用两种方法抑制皮肤癣菌须毛癣菌、红色毛癣菌和疣状毛癣菌、犬小孢子菌和石膏小孢子菌生物膜的形成。第一种方法是使用浓度为 39 至 1000 ppm 的鼠李糖脂，第二种方法是使用浓度为 8、16 和 32 µg/ml 的亚甲基蓝的光动力法。此外，同时对这两种方法进行了评价。使用分光光度法和扫描电子显微镜评估生物膜的形成。生物表面活性剂已被证明具有改善的抑制菌株生物膜形成的能力。虽然光动力疗法尚未成功，但与生物表面活性剂联合使用，可能会产生协同效应。通过研究鼠李糖脂对生物膜形成的影响，发现与毛癣菌相比，小孢子菌对孔表面的附着力相对较强。用电子显微镜对鼠李糖脂和光动力学同时处理的生物膜进行了评估。结果表明，处理后，生物膜变得离散，其结构完整性降低。即使在最具抗性的皮肤真菌之一的小孢子菌属物种中，处理后真菌生物膜的变化也很显着。