Natural pigments are becoming increasingly popular owing of their reliability. Microbial pigments provide an alternative to natural colours. A total of 24 fungal cultures were collected from leaf bits of Senna auriculata, with one strain (FNG1) producing an extracellular red orange pigment. Nigrospora oryzae was confirmed by using physical criteria and molecular phylogenetic study by using ITS and β- tubulin analysis. In EtOAc, the crude red pigment was the most soluble. The TLC analysis was used to partly purify the natural pigment. The partially purified fungal pigment was used in successive bioprospecting studies. The antimicrobial activity of the partially purified sample was assessed against eight human pathogens, with Leucobacter AA7 showing the largest zone of inhibition (200–500 µg/mL). The compound's DPPH scavenging activity enhanced from 38.2 to 67.9%, with an IC₅₀ value of 34.195 ± 2.33 µg/mL. Cancer cells were suppressed by partly pure fungal pigment, but non-cancerous HEK 293 cells were unaffected. The GC–MS analysis was used to characterize the molecule present in the partly purified pigment. In addition, the cotton textiles have the greatest staining capability for crude mycobial pigment, which dyes quickly and has a negative cytotoxicity.

天然颜料由于其可靠性而变得越来越受欢迎。微生物色素提供了天然色素的替代品。从番泻叶叶碎片中收集了总共 24 种真菌培养物，其中一种菌株 (FNG1) 产生细胞外橙红色素。Nigrospora oryzae通过物理标准和 ITS 和 β-微管蛋白分析的分子系统发育研究得到证实。在 EtOAc 中，粗品红色颜料的溶解度最高。TLC分析用于部分纯化天然色素。部分纯化的真菌色素用于连续的生物勘探研究。针对八种人类病原体评估了部分纯化样品的抗菌活性，其中 白杆菌 AA7 显示出最大的抑制区 (200–500 µg/mL)。该化合物的 DPPH 清除活性从 38.2% 增强至 67.9%，IC ₅₀值为 34.195 ± 2.33 µg/mL。部分纯真菌色素可抑制癌细胞，但非癌性 HEK 293 细胞不受影响。GC-MS 分析用于表征部分纯化颜料中存在的分子。此外，棉纺织品对粗真菌色素的染色能力最强，染色速度快，且具有负细胞毒性。