One of the most prevalent infectious diseases identified in both communities and hospitalized patients is urinary tract infection (UTI). Enterococcus is evolved into a clinically pertinent uropathogen due to its evolving resistance to multiple antimicrobial agents.This study, detects antimicrobial susceptibility patterns of Enterococcus species and molecular detection of Enterococcus faecalis from patients with urinary tract infections. In this cross-sectional observational study, 165 urine samples were obtained from clinically diagnosed patients with UTIs of different ages and gender. Enterococcus species were identified by standard microbiological procedure and PCR (by using species-specific primers for Enterococcus faecalis). A modified Kirby Bauer Disc diffusion method was used to identify the antimicrobial susceptibility pattern following Clinical and Laboratory Standards Institute (CLSI) guidelines. Out of 165 urine samples, 134 samples yielded positive cultures. Enterococcus species were isolated from 23 (17.1%) urine samples. Among all Enterococcus, 16 (69.6%) isolates were E. faecalis, detected by PCR assay. A higher (30.4%) proportion of Enterococcus-positive patients were from the age group 48–57 years and female patients (78.2%) had a higher prevalence. Enterococcal infection was found in 56.5% of non-catheterized patients and 43.5% of catheterized patients. Vancomycin and linezolid (78.3%) and meropenem (73.9%) sensitivity was prevalent among all Enterococcus species. They showed 100% resistance towards ceftriaxone, cefixime 95.7%, cefuroxime 91.3%, azithromycin 82.6%. This research indicated the occurrence of Enterococcus species and the advent of multidrug-resistant E. faecalis in patients with UTIs. Routine speciation and antimicrobial susceptibility testing of Enterococcus in various clinical samples is encouraged.

尿路感染 (UTI) 是社区和住院患者中最流行的传染病之一。由于肠球菌对多种抗菌药物不断产生耐药性，它已演变成一种临床相关的尿路病原体。本研究检测尿路感染患者肠球菌的抗菌敏感性模式以及粪肠球菌的分子检测。在这项横断面观察研究中，从临床诊断的不同年龄和性别的尿路感染患者中获取了 165 份尿液样本。通过标准微生物程序和 PCR（通过使用粪肠球菌物种特异性引物）鉴定肠球菌物种。根据临床和实验室标准研究所 (CLSI) 指南，使用改良的柯比鲍尔纸片扩散法来确定抗菌药物敏感性模式。165 份尿液样本中，134 份样本培养结果呈阳性。从 23 份 (17.1%) 尿液样本中分离出肠球菌。在所有肠球菌中，16 株（69.6%）为大肠杆菌。粪肠球菌，通过PCR检测检测。肠球菌阳性患者的比例较高（30.4%）来自48-57岁年龄组，女性患者（78.2%）的患病率较高。56.5% 的未置管患者和 43.5% 的置管患者中发现肠球菌感染。万古霉素和利奈唑胺 (78.3%) 和美罗培南 (73.9%) 敏感性在所有肠球菌属中普遍存在。他们对头孢曲松100%耐药，头孢克肟95.7%，头孢呋辛91.3%，阿奇霉素82.6%。这项研究表明，尿路感染患者中出现了肠球菌属物种和多重耐药粪肠球菌的出现。鼓励对各种临床样本中的肠球菌进行常规形态分析和抗菌药物敏感性测试。