Objective

Increasing evidence shows that microRNA (miR) is related to drug resistance in hepatocellular carcinoma (HCC). miR-34b-5p could retard tumor development, but its function in HCC is still unclear.

Methods

miR-34b-5p expression was determined in HCC tissues and cell lines. Analysis and verification of the binding between miR-34b-5p and metadherin (MTDH) was carried out. The actual action of miR-34b-5p and MTDH in the area of proliferation, apoptosis, invasion, and migration of cisplatin (DDP)-resistant HCC cells was monitored.

Results

miR-34b-5p was downregulated in HCC. Overexpression of miR-34b-5p enhanced the sensitivity of HCC cells to DDP, inhibiting proliferation, migration, and invasion and promoting apoptosis. MTDH was the direct target of miR-34b-5p. MTDH was upregulated in HCC tissues, which was negatively correlated with miR-34b-5p expression. Enhancement of MTDH can reverse the effect of upregulated miR-34b-5p on the chemosensitivity of HCC cells.

Conclusion

miR-34b-5p targets MTDH and enhances the chemosensitivity of HCC cells.

中文翻译：

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MicroRNA-34b-5p增加肝细胞癌细胞的化疗敏感性

客观的

越来越多的证据表明 microRNA (miR) 与肝细胞癌 (HCC) 的耐药性相关。miR-34b-5p可以延缓肿瘤的发展，但其在HCC中的功能仍不清楚。

方法

在 HCC 组织和细胞系中测定了 miR-34b-5p 的表达。对miR-34b-5p和metadherin（MTDH）之间的结合进行了分析和验证。监测 miR-34b-5p 和 MTDH 在顺铂 (DDP) 耐药性 HCC 细胞的增殖、凋亡、侵袭和迁移方面的实际作用。

结果

miR-34b-5p 在 HCC 中下调。miR-34b-5p的过表达增强了HCC细胞对DDP的敏感性，抑制增殖、迁移和侵袭并促进细胞凋亡。MTDH 是 miR-34b-5p 的直接靶标。MTDH 在 HCC 组织中表达上调，与 miR-34b-5p 表达呈负相关。MTDH的增强可以逆转上调的miR-34b-5p对HCC细胞化疗敏感性的影响。

结论

miR-34b-5p 靶向 MTDH 并增强 HCC 细胞的化疗敏感性。