C-C motif chemokine ligand 2 (CCL2) has been reported to be expressed in the bovine endometrium during pregnancy. However, the details of its functions involved in the implantation mechanism are still not clear. The purpose of this study is to analyze the functional properties of CCL2 in the bovine endometrium and embryos. The expression of CCR2 was not different between the luteal phase and implantation phase of their endometrial tissues, but was significantly high in IFNa treated bovine endometrial stromal (BES) cells in vitro. The expressions of PGES1, PGES2, AKR1C4, and AKR1C4 were high at the implantation stage compared with the luteal stage. On the other hand, PGES2 and AKR1B1 in BEE and PGES3 and AKR1A1 in BES were significantly increased by CCL2 treatment, respectively. The expressions of PCNA and IFNt were found significantly high in the bovine trophoblastic cells (BT) treated with CCL2 compared to the control. CCL2 significantly increased the attachment rate of BT vesicles to BEE in in vitro co-culture system. The expression of OPN and ICAM-1 increased in BEE, and ICAM-1 increased in BT by CCL2 treatment, respectively. The present results indicate that CCL2 has the potential to regulate the synthesis of PGs in the endometrium and the embryo growth. In addition, CCL2 has the possibility to regulate the process of bovine embryo attachment to the endometrium by modulation of binding molecules expression.

据报道，CC 基序趋化因子配体 2 (CCL2) 在妊娠期间的牛子宫内膜中表达。然而，其在植入机制中所涉及的功能细节仍不清楚。本研究的目的是分析 CCL2 在牛子宫内膜和胚胎中的功能特性。CCR2的表达在其子宫内膜组织的黄体期和着床期之间没有差异，但在体外IFNa处理的牛子宫内膜基质（BES）细胞中显着高。与黄体期相比， PGES1、PGES2、AKR1C4和AKR1C4在着床期的表达较高。另一方面， CCL2处理分别显着增加了BEE中的PGES2和AKR1B1以及BES中的PGES3和AKR1A1。与对照组相比，用 CCL2 处理的牛滋养层细胞 (BT) 中PCNA和IFNt的表达显着升高。在体外共培养系统中，CCL2 显着增加了 BT 囊泡与 BEE 的附着率。通过CCL2处理，BEE中OPN和ICAM-1的表达增加， BT中ICAM-1的表达增加。目前的结果表明，CCL2 具有调节子宫内膜中 PG 的合成和胚胎生长的潜力。此外，CCL2有可能通过调节结合分子的表达来调节牛胚胎附着到子宫内膜的过程。