Ficus tikoua Bureau is an ecologically functional perennial vine that belongs to the Moraceae family. Tissue culture is a common method for the rapid propagation of plants and is suitable for the rapid propagation of F. tikoua. The objective of the current study was to evaluate the process of F. tikoua organogenesis in vitro utilizing stem explants. An efficient tissue culture propagation method was developed by manipulating several plant growth regulators (PGRs) and their proportions, as well as evaluating alternative medium options for specific phases of differentiation. Explants were cultured on MS medium, supplemented with various thidiazuron (TDZ) concentrations with 6-benzylaminopurine (BA) and naphthaleneacetic acid (NAA), to investigate their efficacy during in vitro shoot organogenesis. The results demonstrated that the combination of 0.5 mg L⁻¹ BA, 1.0 mg L⁻¹ NAA, and 1.0 mg L⁻¹ TDZ was the most effective for callus formation (91.1%) and that the combination of 2.0 mg L⁻¹ BA and 0.5 mg L⁻¹ TDZ was the most suited for adventitious shoot induction (61.11%). The medium supplemented with 1.0 mg L⁻¹ BA, 1.0 mg L⁻¹ NAA, and 0.5 mg L⁻¹ TDZ yielded the highest bud multiplication number with a 6.05 proliferation factor. The cultured shoots rooted easily on half-strength MS medium containing 1.0 mg L⁻¹ IBA and 0.1 mg L⁻¹ NAA (68.52%). The subsequent regenerated plants were grown in a mixture of garden soil, perlite, and vermiculite in a ratio of 2:1:1, resulting in a survival rate of 86.17%.

Ficus tikoua Bureau 是一种具有生态功能的多年生藤本植物，属于桑科。组织培养是植物快速繁殖的常用方法，适合F. tikoua的快速繁殖。本研究的目的是利用茎外植体评估F. tikoua器官发生的过程。通过操纵几种植物生长调节剂（PGR）及其比例，以及评估特定分化阶段的替代培养基选项，开发了一种有效的组织培养繁殖方法。外植体在 MS 培养基上培养，并补充不同浓度的噻苯隆 (TDZ)、6-苄氨基嘌呤 (BA) 和萘乙酸 (NAA)，以研究它们在体外芽器官发生过程中的功效。结果表明，0.5 mg L ^-1 BA、1.0 mg L ^-1 NAA 和 1.0 mg L ^-1 TDZ 的组合对愈伤组织形成最有效（91.1%），2.0 mg L ^-1 BA的组合对愈伤组织形成最有效。 0.5 mg L ^-1 TDZ 最适合不定芽诱导（61.11%）。补充有1.0 mg L ^-1 BA、1.0 mg L ^-1 NAA和0.5 mg L ^-1 TDZ的培养基产生最高的芽增殖数，增殖因子为6.05。^{培养的芽在含有1.0 mg L -1} IBA和0.1 mg L ^-1 NAA的半强度MS培养基上容易生根（68.52%）。随后的再生植物在花园土、珍珠岩和蛭石按2:1:1的比例混合生长时，成活率为86.17%。