Candida boidinii is a methylotrophic yeast with wide geographical distribution. In the present study, the microorganism was isolated from the Bahian semiarid and the enzymatic extract containing β-glucosidase was obtained through submerged fermentation. Response surface methodology was employed to optimize of fermentation medium. The higher production of β-glucosidase was obtained after 71 h of fermentation in an optimized medium composed of 3.35% glucose, 1.78% yeast extract and 1% peptone. The optimum pH and temperature of enzymatic activity were 6.8 (citrate–phosphate buffer) and 71.7 °C, respectively. Salts tested (10 mM) CaCl₂, Na₂SO₄ and ZnSO₄ promotes the increase of 91%, 45% and 80% of activity, respectively. The enzyme retained 64% ± 2.3 of its initial activity after 1 h heating at 90 °C. The production of reducing sugars was 95.94% after 24 h of hydrolysis and, with the addition of metal ions, this value increased more than 2 times. Among the supports analyzed for immobilization, chitosan showed higher residual activity during reuse. The immobilized enzyme showed higher activity at 60 °C with pH 6 and preserved almost 100% of the initial activity after 30 min at 70 °C.

博伊丁假丝酵母是一种地理分布广泛的甲基营养酵母。本研究从巴伊安半干旱地区分离出该微生物，通过深层发酵获得含有β-葡萄糖苷酶的酶提取物。采用响应面法对发酵培养基进行优化。在由3.35%葡萄糖、1.78%酵母提取物和1%蛋白胨组成的优化培养基中发酵71 h后，获得了较高的β-葡萄糖苷酶产量。酶活性的最适pH和温度分别为6.8（柠檬酸盐-磷酸盐缓冲液）和71.7℃。测试的盐(10 mM) CaCl ₂、Na ₂ SO ₄和ZnSO ₄分别促进活性增加91%、45%和80%。在 90 °C 加热 1 小时后，该酶保留了其初始活性的 64% ± 2.3。水解24小时后，还原糖的生成率为95.94%，随着金属离子的添加​​，该值增加了2倍以上。在分析固定化的载体中，壳聚糖在再利用过程中表现出较高的残留活性。固定化酶在 60 °C、pH 6 时表现出较高的活性，并且在 70 °C 30 分钟后几乎保留了 100% 的初始活性。