Abstract

The review discusses electrochemical methods for analysis of drug interactions with DNA. The electroanalysis method is based on the registration of interaction-induced changes in the electrochemical oxidation potential of heterocyclic nitrogenous bases in the DNA molecule and in the maximum oxidation current amplitude. The mechanisms of DNA–drug interactions can be identified based on the shift in the electrooxidation potential of heterocyclic nitrogenous bases toward more negative (cathodic) or positive (anodic) values. Drug intercalation into DNA shifts the electrochemical oxidation potential to positive values, indicating thermodynamically unfavorable process that hinders oxidation of nitrogenous bases in DNA. The potential shift toward the negative values indicates electrostatic interactions, e.g., drug binding in the DNA minor groove, since this process does not interfere with the electrochemical oxidation of bases. The concentration-dependent decrease in the intensity of electrochemical oxidation of DNA bases allows to quantify the type of interaction and calculate the binding constants.

中文翻译：

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药物基因组学研究中的电分析：药物与 DNA 相互作用的机制

摘要

该综述讨论了分析药物与 DNA 相互作用的电化学方法。电分析方法基于记录相互作用引起的DNA分子中杂环含氮碱基的电化学氧化电位和最大氧化电流幅度的变化。DNA-药物相互作用的机制可以根据杂环含氮碱基的电氧化电位向负值（阴极）或正值（阳极）的转变来确定。药物嵌入 DNA 后，电化学氧化电位变为正值，这表明热力学上不利的过程会阻碍 DNA 中含氮碱基的氧化。电位向负值的转变表明静电相互作用，例如DNA小沟中的药物结合，因为该过程不干扰碱基的电化学氧化。DNA 碱基电化学氧化强度的浓度依赖性降低可以量化相互作用的类型并计算结合常数。