Abstract

This review highlights operational principles, features, and modern aspects of the development of third-generation sequencing technology of biopolymers focusing on the nucleic acids analysis, namely the nanopore sequencing system. Basics of the method and technical solutions used for its realization are considered, from the first works showing the possibility of creation of these systems to the easy-to-handle procedure developed by Oxford Nanopore Technologies company. Moreover, this review focuses on applications, which were developed and realized using equipment developed by the Oxford Nanopore Technologies, including assembly of whole genomes, methagenomics, direct analysis of the presence of modified bases.

中文翻译：

---

使用纳米孔测序直接测定单一生物聚合物分子的结构

摘要

本综述重点介绍了专注于核酸分析的第三代生物聚合物测序技术（即纳米孔测序系统）发展的操作原理、特点和现代方面。考虑了用于其实现的方法和技术解决方案的基础知识，从显示创建这些系统的可能性的第一个作品到牛津纳米孔技术公司开发的易于处理的程序。此外，本综述重点关注使用牛津纳米孔技术公司开发的设备开发和实现的应用，包括全基因组组装、宏基因组学、修饰碱基存在的直接分析。