Abstract

The review considers the problem of hydrogen peroxide decomposition and hydroxyl radical formation in the presence of iron in vivo and in vitro. Analysis of the literature data allows us to conclude that, under physiological conditions, transport of iron, carried out with the help of carrier proteins, minimizes the possibility of appearance of free iron ions in cytoplasm of the cell. Under pathological conditions, when the process of transferring an iron ion from a donor protein to an acceptor protein can be disrupted due to modifications of the carrier proteins, iron ions can enter cytosol. However, at pH values close to neutral, which is typical for cytosol, iron ions are converted into water-insoluble hydroxides. This makes it impossible to decompose hydrogen peroxide according to the mechanism of the classical Fenton reaction. A similar situation is observed in vitro, since buffers with pH close to neutral are used to simulate free radical oxidation. At the same time, iron hydroxides are able to catalyze decomposition of hydrogen peroxide with formation of a hydroxyl radical. Decomposition of hydrogen peroxide with iron hydroxides is called Fenton-like reaction. Studying the features of Fenton-like reaction in biological systems is the subject of future research.

中文翻译：

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体内和体外芬顿反应。可能性和局限性

摘要

该综述考虑了体内和体外铁存在下过氧化氢分解和羟基自由基形成的问题。对文献数据的分析使我们得出这样的结论：在生理条件下，在载体蛋白的帮助下进行的铁转运最大限度地减少了细胞细胞质中出现游离铁离子的可能性。在病理条件下，当铁离子从供体蛋白转移到受体蛋白的过程由于载体蛋白的修饰而被破坏时，铁离子可以进入细胞质。然而，在接近中性的 pH 值（细胞质的典型 pH 值）下，铁离子会转化为不溶于水的氢氧化物。这使得过氧化氢无法按照经典芬顿反应的机理分解。在体外也观察到类似的情况，因为使用 pH 值接近中性的缓冲液来模拟自由基氧化。同时，氢氧化铁能够催化过氧化氢分解并形成羟基自由基。过氧化氢与氢氧化铁的分解称为类芬顿反应。研究生物系统中类芬顿反应的特征是未来研究的主题。