Sea buckthorn (Hippophae rhamnoides), a hardy deciduous shrub of the family Elaeagnaceae, is known for its economic and ecological importance due to its wide use in medicine, food, windbreaks, and sand fixation. In this study, the first genetic linkage map of H. rhamnoides was constructed based on an F₁ population (H. rhamnoides subsp. mongolica ‘wulangemu’ × H. rhamnoides subsp. sinensis ‘wucixiong’) by using start codon targeted (SCoT), sequence-related amplified polymorphism (SRAP), and simple sequence repeat (SSR) markers. The linkage map contained 1143 markers with a total length of 1889.46 centimorgans (cM), including 12 linkage groups (LG) and covering 97.17% of the estimated genome, with an average marker density of 1.67 cM. According to the phenotypic variation analysis, 61 QTLs were identified. Among these QTLs, there were 18 QTLs for seedling height, 27 QTLs for ground diameter, two QTLs for primary branching angle, 11 QTLs for leaf width, and 3 QTLs for leaf shape index. The range of phenotypic variation explained by these QTLs was 10.7 ~ 21.9%. The QTLs identified by high-density linkage mapping reported in this study will be useful for the molecular breeding of sea buckthorn.

沙棘（Hippophae rhamnoides）是胡颓子科的一种耐寒落叶灌木，因其在医药、食品、防风和固沙方面的广泛用途而以其经济和生态重要性而闻名。本研究基于F ₁群体（H. rhamnoides subsp. mongolica 'wulangemu' × H. rhamnoides subsp. sinensis 'wucixiong'），利用起始密码子靶向（SCoT）构建了第一个沙棘遗传连锁图谱。 、序列相关扩增多态性 (SRAP) 和简单序列重复 (SSR) 标记。连锁图谱包含1143个标记，总长度为1889.46厘摩(cM)，其中包括12个连锁群(LG)，覆盖了估计基因组的97.17%，平均标记密度为1.67 cM。根据表型变异分析，鉴定出61个QTL。其中，苗高QTL 18 个，地径QTL 27 个，初分枝角度QTL 2 个，叶宽QTL 11 个，叶形指数QTL 3 个。这些QTL解释的表型变异范围为10.7%~21.9%。本研究报道的高密度连锁图谱鉴定的QTL将有助于沙棘的分子育种。