Bark residues from Douglas fir are an abundant resource that is currently used primarily in low-value energy recovery or is landfilled. Bark extractives are rich in diverse compounds like terpenes, fatty acids, phenols, and sugars with potential uses in a variety of high value applications. The study explores the potential of enzymatic hydrolysis to improve phenolic compounds from Douglas fir bark. It also assesses differences in chemical composition among rhytidome, phloem, and comingled bark fractions from an industrial waste pile. Phloem fractions exhibit higher yields of extractives, rhytidome fractions have elevated lignin levels, while the comingled fraction lies between the two except in ash content which was higher than in the separated fractions. Fungal decay tests with Gloeophyllum trabeum and Coniophora puteana on extract treated wood suggest potential for growth inhibition in extracts, about 58–31 % and 30–7% mass loss (in average) respectively, but due to high mass loss at low concentrations an enzymatic modification approach seems crucial for enhanced inhibition. Growth responses in whole-cell fermentation approach display variability depending on the participating microorganisms. Enzymatic hydrolysis with beta-glucosidase improved the antioxidant properties of bark extracts and holds promise for altering the chemical composition and enhancing bioactivity.

中文翻译：

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花旗松树皮：成分、提取物利用和酶处理以富集生物活性成分

花旗松的树皮残留物是一种丰富的资源，目前主要用于低价值能源回收或被填埋。树皮提取物富含萜烯、脂肪酸、酚和糖等多种化合物，在各种高价值应用中具有潜在用途。该研究探讨了酶水解改善花旗松树皮酚类化合物的潜力。它还评估了工业废物堆中的皱纹、韧皮部和混合树皮部分之间化学成分的差异。韧皮部部分表现出较高的提取物收率，皱纹部分具有较高的木质素水平，而混合部分除了灰分含量高于分离部分外，位于两者之间。真菌腐烂测试石竹和玉果经提取物处理的木材表明提取物具有潜在的生长抑制作用，质量损失（平均）分别约为 58-31% 和 30-7%，但由于低浓度下的质量损失较大，酶促修饰方法似乎对于增强抑制至关重要。全细胞发酵方法中的生长反应根据参与的微生物而表现出变异性。使用 β-葡萄糖苷酶进行酶水解可改善树皮提取物的抗氧化特性，并有望改变化学成分并增强生物活性。