Abstract

REVEILLE (RVEs) transcription factors play an important role in regulating circadian clock, plant growth and development, and stress responses. While RVEs have been identified in various plant species, comprehensive genome exploration and analysis of RVEs in castor (Ricinus communis L.) have not been previously reported. In this study, we identified RcRVEs in the castor genome through sequence alignment and conducted an analysis encompassing gene structures, protein motifs, physicochemical properties, transcription patterns under cold stress, different tissues, and diurnal rhythm. A total of five RcRVEs were pinpointed in the castor genome. Evolutionary tree analysis categorized these RcRVEs into three distinct groups, implying that different RcRVEs may fulfill distinct biological functions. Furthermore, several abiotic stresses and hormone-responsive cis-acting elements were found in the promoters of the RcRVEs. RNA-sequencing (RNA-seq) analysis revealed that RcRVE1 and RcRVE7 were induced by cold stress and exhibited upregulation over time, indicating their involvement in regulating cold adaptation in castor plants. Five RcRVEs have notably higher expression levels in leaves compared to other tissues. The expression of all RcRVEs displayed diurnal rhythms under light/dark cycles. This study provides valuable insights into the potential functions of RcRVEs in regulating castor’s growth and development, and responses to cold stress.

中文翻译：

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蓖麻 REVEILLE 转录因子的全基因组鉴定和表达模式分析

摘要

REVEILLE (RVEs) 转录因子在调节生物钟、植物生长发育以及应激反应中发挥着重要作用。虽然 RVE 已在多种植物物种中被发现，但此前尚未报道过蓖麻 ( Ricinus communis L.) 中 RVE 的全面基因组探索和分析。在本研究中，我们通过序列比对鉴定了蓖麻基因组中的RcRVE，并对基因结构、蛋白质基序、理化特性、冷应激下的转录模式、不同组织和昼夜节律进行了分析。在蓖麻基因组中总共定位到了 5 个RcRVE 。进化树分析将这些 RcRVE 分为三个不同的组，这意味着不同的 RcRVE 可能实现不同的生物学功能。此外，在RcRVE的启动子中发现了一些非生物胁迫和激素响应性顺式作用元件。RNA测序（RNA-seq）分析表明，RcRVE1和RcRVE7是由冷胁迫诱导的，并随着时间的推移表现出上调，表明它们参与调节蓖麻植物的冷适应。与其他组织相比，五个RcRVE在叶子中的表达水平明显更高。所有RcRVE的表达在光/暗循环下均表现出昼夜节律。这项研究为 RcRVE 在调节蓖麻生长发育以及冷应激反应方面的潜在功能提供了宝贵的见解。