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Field incidence and analysis of biology and morphometrics of the invasive spiralling whitefly, Aleurodicus dispersus (Hemiptera: Aleyrodidae), in cotton in Tamil Nadu, India
International Journal of Tropical Insect Science ( IF 1.2 ) Pub Date : 2024-03-14 , DOI: 10.1007/s42690-024-01205-9
V Sadhana , K Senguttuvan , M Murugan , C Yogambal , T Ramasubramanian , R Caroline Nirmala , M Jayakanthan

The incidence of Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae) was surveyed in production fields of cotton, Gossypium hirsutum L., in Tamil Nadu, India, from December 2021 to April 2022. Plants sampled in the crops were winter and summer planted, 45–95 days past seeding, and in the flowering to boll development stages. Whitefly counts from the sampling yielded a range of 4.4 to 8.9 adults and nymphs per 3 leaves (sample unit). The nymphs and adults collected from the fields were taxonomically confirmed using taxonomic characteristics obtained by photographs of live and slide-mounted specimens. Identification was further confirmed by molecular techniques using the mitochondrial Cytochrome Oxidase subunit I gene (COI) universal primer-based polymerase chain reaction (PCR) amplification and sequencing. The resulting dendrogram, constructed using MEGA-X and analyzed using the maximum likelihood method, yielded a single clade with sub-lineages of the specimens collected in this survey. Overall, the mean (± SD) duration of the entire life cycle of A. dispersus collected was 43.9 ± 2.4 d. Mean (± SD) length x width the developmental stages were 283.8 ± 13.3 μm x 167.0 ± 4.5 μm for eggs, 335.7 ± 19.3 μm x 266.9 ± 25.7 μm for first instars, 473.8 ± 21.6 μm x 349.8 ± 21.3 μm for second instars, 774.4 ± 15.11 μm x 440.2 ± 5.8 μm for third instars, 881.1 ± 16.7 μm x 634.7 ± 5.2 μm for fourth instars, and 970.0 ± 9.1 μm x 836.9 ± 12.7 μm for adults.



中文翻译:

印度泰米尔纳德邦棉花中入侵性螺旋粉虱 Aleurodicus 分散粉虱(半翅目:粉虱科)的现场发生率及生物学和形态测量分析

2021 年 12 月至 2022 年 4 月,对印度泰米尔纳德邦的棉花( Gossypium hirsutum L. )生产田中的粉虱(半翅目:粉虱科)的发病率进行了调查。农作物中采样的植物是冬季和夏季种植的,45-播种后95天,处于开花至棉铃发育阶段。采样中的粉虱计数为每 3 片叶子(样本单位)4.4 至 8.9 只成虫和若虫。使用通过活体标本和载玻片标本照片获得的分类学特征对从田间收集的若虫和成虫进行分类学确认。使用基于线粒体细胞色素氧化酶亚基 I 基因 (COI) 通用引物的聚合酶链式反应 (PCR) 扩增和测序的分子技术进一步证实了鉴定。使用 MEGA-X 构建并使用最大似然法分析所得的树状图,产生了一个具有本次调查中收集的样本子谱系的单一进化枝。总体而言,所收集的分散香苋整个生命周期的平均(± SD)持续时间为 43.9 ± 2.4 d。发育阶段的平均(±SD)长x宽卵为283.8±13.3μm x 167.0±4.5μm,第一龄为335.7±19.3μm x 266.9±25.7μm,第二龄为473.8±21.6μm x 349.8±21.3μm,第三龄为774.4±15.11μm×440.2±5.8μm,第四龄为881.1±16.7μm×634.7±5.2μm,成虫为970.0±9.1μm×836.9±12.7μm。

更新日期:2024-03-15
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