Abstract

Dental infections and systemic complications caused by Streptococcus species in the oral cavity are increasingly exhibiting resistance to commonly used antibiotics, posing a potential threat to global public health. Phage therapy may offer a superior alternative, given that bacteriophages can be easily isolated and rapidly replicate in large numbers. In this study, six Streptococcus species from the oral cavity were characterized. Bacteriophages isolated from wastewater using five of these species as hosts produced plaques ranging from 0.2 to 2.4 mm in size. The phages demonstrated stability within a temperature range of 4 ℃ to 37 ℃. However, at temperatures exceeding 45 ℃, a noticeable reduction in bacteriophage titer was observed. Similarly, the phages showed greater stability within a pH range of 5 to 10. The isolated phages exhibited latency periods ranging from 15 to 20 min and had burst sizes varying from 10 to 200 viral particles. This study supports the potential use of bacteriophages in controlling infections caused by Streptococcus species.

中文翻译：

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口腔中针对链球菌属的噬菌体的分离可潜在地控制牙科疾病和相关的全身并发症

摘要

口腔中链球菌引起的牙齿感染和全身并发症越来越多地表现出对常用抗生素的耐药性，对全球公共卫生构成潜在威胁。鉴于噬菌体可以轻松分离并快速大量复制，噬菌体疗法可能提供一种更好的选择。在这项研究中，对口腔中的六种链球菌进行了鉴定。使用其中五种细菌作为宿主从废水中分离出噬菌体，产生大小为 0.2 至 2.4 毫米的噬菌斑。噬菌体在4℃至37℃的温度范围内表现出稳定性。然而，当温度超过45℃时，噬菌体滴度明显降低。同样，噬菌体在 5 至 10 的 pH 范围内表现出更高的稳定性。分离的噬菌体表现出 15 至 20 分钟的潜伏期，爆发大小为 10 至 200 个病毒颗粒。这项研究支持噬菌体在控制链球菌引起的感染方面的潜在用途。