Abstract

The cadherin superfamily molecules, functioning as cell adhesion molecules, are recognized to play roles in both physiological and pathological processes. The cadherin-based adherent junction (CAJ) is believed to interact with presenilin-1 (PS-1), suggesting that disruptions in CAJ structures might contribute to neurodegeneration, potentially leading to Alzheimer’s Disease (AD). Yet, the specific expression patterns of cadherin superfamily mRNA remain somewhat ambiguous. This research utilized in situ hybridization (ISH) to examine the expression and localization of cadherin mRNA in AD mouse model brains. Long cRNA probes targeting cadherin revealed endogenous mRNA expression in brain sections. Interestingly, senile plaques in the AD mouse brain were also bound to these probes. This binding, however, did not exclusively denote cadherin mRNA, as ISH detected both antisense and sense cRNA probes. Our data suggest that although antisense cRNA probes effectively detected cadherin mRNA expression in AD brain cells, their association with senile plaques may not specifically indicate cadherin mRNA expression.

中文翻译：

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钙粘蛋白cRNA探针在阿尔茨海默病小鼠模型脑中的应用

摘要

钙粘蛋白超家族分子作为细胞粘附分子，被认为在生理和病理过程中发挥作用。基于钙粘蛋白的粘附连接 (CAJ) 被认为与早老素-1 (PS-1) 相互作用，这表明 CAJ 结构的破坏可能会导致神经退行性变，从而可能导致阿尔茨海默病 (AD)。然而，钙粘蛋白超家族 mRNA 的具体表达模式仍然有些模糊。本研究利用原位杂交 (ISH) 检测 AD 小鼠模型大脑中钙粘蛋白 mRNA 的表达和定位。靶向钙粘蛋白的长 cRNA 探针揭示了脑切片中的内源 mRNA 表达。有趣的是，AD 小鼠大脑中的老年斑也与这些探针结合。然而，这种结合并不专门表示钙粘蛋白 mRNA，因为 ISH 检测到了反义和有义 cRNA 探针。我们的数据表明，虽然反义 cRNA 探针有效检测 AD 脑细胞中钙粘蛋白 mRNA 的表达，但它们与老年斑的关联可能无法特异性指示钙粘蛋白 mRNA 的表达。