Laccase is an extracellular enzyme that is widely used in the decolonization of textile dyes in waste water. The aim of our study was to isolate, purify, characterize and immobilize the laccase enzyme produced by Trametes versicolor HBB 7328. Purified laccase enzyme was immobilized in polyacrylamide gel to explore its ability in decolonization of textile dyes. Laccase purification process was carried out by fractionation using ammonium sulphate (80%) followed by DEAE Sepharose column (30 × 3 cm) chromatography method. Recovery and fold purification in this step were 27.35 and 16.23%. Purified laccase (named as LAC1) revealed its optimum activity at pH 5.0 and 35 °C temperature, and displayed remarkable stability in the range of 30–40 °C and in the pH range (pH 3.0–7.0). The single bands on SDS-PAGE represent the purity of LAC1 with molecular weight of 60 kDa. Both free and immobilized laccase assessed for their ability to decolorize textile dyes. Free laccase decolorized Methyl red to 72.705%, Reactive orange to 57.851%, Reactive blue to 37.231%, Bromophenol blue to 24.412% however Immobilized laccase decolorized Methyl red to 89.823%, Reactive orange to 63.151%, Reactive blue to 59.548%, Bromophenol blue to 49.421% respectively. This study proposes the role of laccase from Trametes versicolor HBB 7328 in decolonization of textile dyes.

Graphical Abstract

中文翻译：

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栓菌 HBB 7328 漆酶的制备、纯化和固定化及其在纺织染料脱色中的作用

漆酶是一种胞外酶，广泛用于废水中纺织染料的去殖民化。我们的研究目的是分离、纯化、表征和固定由Trametes versicolor HBB 7328产生的漆酶。将纯化的漆酶固定在聚丙烯酰胺凝胶中，以探索其对纺织染料去殖民化的能力。漆酶纯化过程通过使用硫酸铵（80%）分馏，然后通过 DEAE Sepharose 柱（30 × 3 cm）层析方法进行。该步骤的回收率和纯化倍数分别为27.35%和16.23%。纯化的漆酶（命名为 LAC1）在 pH 5.0 和 35 °C 温度下表现出最佳活性，并在 30–40 °C 范围和 pH 范围（pH 3.0–7.0）内表现出显着的稳定性。 SDS-PAGE 上的单条带代表分子量为 60 kDa 的 LAC1 的纯度。评估了游离漆酶和固定化漆酶对纺织染料脱色的能力。游离漆酶脱色 甲基红至 72.705%，活性橙至 57.851%，活性蓝至 37.231%，溴酚蓝至 24.412% 然而 固定化漆酶脱色 甲基红至 89.823%，活性橙至 63.151%，活性蓝至 59.548%，溴酚蓝分别为 49.421%。本研究提出了来自Trametes versicolor HBB 7328 的漆酶在纺织染料去殖民化中的作用。

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