Abstract

A thermo-alkali stable cellulase from Geobacillus sp. TP-1 was isolated from Tapovan hot spring soil sample. The BLASTn sequence analysis of 16S rRNA sequence revealed that the isolate belonged to the Geobacillus genus and shared the highest degree of sequence similarity (99.43%) with the different strains of Geobacillus subterraneus. The neighbour joining method of multiple sequence alignment revealed that the 16S rRNA sequence of Geobacillus sp. TP-1 shows maximum similarity with Geobacillus stearothermophilus strain S_YE6-1017-022. One-Factor-At-a-Time analysis was used to optimize the carbon source, nitrogen source, pH, temperature, inoculum size and growth profile with respect to cellulase production. When compared to un-optimized basal media, optimised medium increased cellulase production by around 3.6 times. The Plackett Burman factorial design was employed to identify the critical medium components influencing cellulase activity and temperature was determined to have a significant effect on overall cellulase production. The current strain was capable of utilising lignocellulosic waste as an alternative carbon source. The use of sugarcane molasses and wheat bran as carbon sources resulted in a significant increase (~ 7.2 fold) in cellulase production in the current study, indicating the bacterium’s potential for valorising lignocellulosic biomass into value-added products, which encourages its use in lignocellulosic-based bio refineries.

中文翻译：

---

嗜热地芽孢杆菌的耐热纤维素酶生产条件的统计模型。 TP-1 分离自印度 Garhwal 喜马拉雅山脉 Tapovan 温泉

摘要

来自土芽孢杆菌属的热碱稳定纤维素酶。 TP-1是从Tapovan温泉土壤样品中分离出来的。 16S rRNA序列BLASTn序列分析表明，该菌株属于土芽孢杆菌属，与地下土芽孢杆菌不同菌株的序列相似度最高（99.43%） 。多重序列比对的邻接方法揭示了Geobacillus sp.的16S rRNA序列。 TP-1与嗜热脂肪地芽孢杆菌菌株S_YE6-1017-022表现出最大的相似性。使用一次一因素分析来优化纤维素酶生产的碳源、氮源、pH、温度、接种量和生长曲线。与未优化的基础培养基相比，优化的培养基使纤维素酶产量增加了约 3.6 倍。采用 Plackett Burman 因子设计来确定影响纤维素酶活性的关键培养基成分，并确定温度对整个纤维素酶生产有显着影响。目前的菌株能够利用木质纤维素废物作为替代碳源。在当前的研究中，使用甘蔗糖蜜和麦麸作为碳源导致纤维素酶产量显着增加（〜7.2倍），表明该细菌具有将木质纤维素生物质转化为增值产品的潜力，这鼓励其在木质纤维素中的使用为基础的生物精炼厂。