The increasing number of plastic debris produced worldwide and its detrimental impacts on the environment and human health have led to rising demand for bio-plastic, or polyhydroxyalkanoate, as a replacement to synthetic plastic manufactured from petroleum. The usefulness of agricultural waste as a prime source of carbon for Polyhydroxyalkanoate production and aids in the valorization of the waste. The study optimizes sulfuric acid pre-treated wheat straw (SAPWS) along with inoculum size, temperature, and incubation period using Box-Behnken design (BBD). The maximum Polyhydroxyalkanoate (PHA) yield (0.250 g/L) and productivity (0.521%) by Bacillus paranthracis RSKS-3. were found at 10 g/L SAPWS, 5% inoculum size, 7.4 pH, and 48 h of the incubation period. The observed estimations were in statistical accord with the anticipated values of 0.247 g/L and 0.508%. Based on PHA production using SAPWS as a carbon source, 1.2 g/L was produced with was 5.29-fold increment as compared to standard glucose (10 g/L) that produced 0.34 g/L PHA. The characteristic properties of extracted PHA were similar to the standard PHA. Conclusively, agricultural waste possibly will be utilized as an alternative and a renewable feedstock for green synthesis of PHA production.

Graphical Abstract

中文翻译：

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使用硫酸预处理的麦秆 (SAPWS) 作为原料生产聚羟基脂肪酸酯：使用副炭疽杆菌 RSKS-3 进行基于 RSM（响应面方法）的优化

全球产生的塑料碎片数量不断增加，及其对环境和人类健康的不利影响，导致对生物塑料或聚羟基脂肪酸酯的需求不断增长，以替代石油制造的合成塑料。农业废物作为聚羟基脂肪酸酯生产的主要碳源的用途，有助于废物的增值。该研究使用 Box-Behnken 设计 (BBD) 优化了硫酸预处理麦秆 (SAPWS) 以及接种量、温度和培养期。副炭疽杆菌RSKS-3的最大聚羟基脂肪酸酯 (PHA) 产量 (0.250 g/L) 和生产率 (0.521%) 。在 10 g/L SAPWS、5% 接种量、7.4 pH 和 48 小时孵育期下发现。观察到的估计值与 0.247 g/L 和 0.508% 的预期值在统计上一致。基于使用 SAPWS 作为碳源的 PHA 生产，生产了 1.2 g/L，与生产 0.34 g/L PHA 的标准葡萄糖 (10 g/L) 相比，增加了 5.29 倍。提取的 PHA 的特性与标准 PHA 相似。总之，农业废弃物可能会被用作绿色合成 PHA 生产的替代品和可再生原料。

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