Nano-dispersed cerium dioxide is promising for use in medicine due to its unique physicochemical properties, including low toxicity, the safety of in vivo usage, active participation in different redox processes occurring in living cells, and its regenerative potential, manifested in the ability of CeO₂ to participate repeatedly in redox reactions. In this work, we examined the biological activity of cerium dioxide nanoparticles (CeO₂ NPs) synthesized by precipitation in mixed water/alcohol solutions at a constant pH of 9. This synthesis method allowed controlling the size and Ce³⁺/Ce⁴⁺ proportion on the surface of NPs, changing the synthesis conditions and obtaining highly stable suspensions of “naked” CeO₂ NPs. Changes in the surface properties upon contact of CeO₂ NPs with protein-rich media, e.g., bovine serum albumin and DMEM cell culture medium supplemented with 10% fetal bovine serum, the characteristics of nanoparticle uptake by mouse aortic endothelial cells and the antioxidant activity of the nanoparticles of different sizes were investigated by various state-of-the-art analytical methods.

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二氧化铈纳米颗粒的生物活性与尺寸和表面特征的关系

纳米分散二氧化铈由于其独特的理化性质，包括低毒性、体内使用安全、积极参与活细胞中发生的不同氧化还原过程以及其再生潜力，在医学上的应用前景广阔。 CeO ₂反复参与氧化还原反应。在这项工作中，我们检测了在 pH 值恒定为 9 的混合水/酒精溶液中沉淀合成的二氧化铈纳米颗粒 (CeO ₂ NP)的生物活性。这种合成方法可以控制尺寸和 Ce ³⁺ /Ce ⁴⁺比例改变合成条件，获得“裸露”CeO ₂纳米粒子的高度稳定的悬浮液。 CeO ₂ NPs与富含蛋白质的培养基（如牛血清白蛋白和添加10%胎牛血清的DMEM细胞培养基）接触后表面性质的变化、小鼠主动脉内皮细胞摄取纳米颗粒的特性以及CeO 2 NPs的抗氧化活性通过各种最先进的分析方法对不同尺寸的纳米颗粒进行了研究。