Abstract

The present study was aimed at the evaluation of neuroprotective ability of methanolic extract of Trianthema decandra (METD) against hyperglycemia-related cognitive impairment in rats. The extract of T. decandra was standardized by TLC and HPTLC methods. To verify the identity and purity of isolated compounds, they were segregated and characterized using various techniques, including UV-visible spectrophotometry, FT-IR, H-NMR, and Mass spectroscopy. α-Amylase and α-glucosidase inhibition property of the extracts were assessed in-vitro. The screening of the neuroprotective effects of METD in hyperglycemic rats was done utilizing Morri’s water (MWM) and elevated plus maze (EPM) model, as well as acetylcholinesterase (AChE) activity. The extracts of Trianthema decandra and its chemical constituents, namely quercetin and phytol, demonstrated a significant protective effect on enzymes like α-amylase and α-glucosidase. Methanol and hydroalcoholic extracts have shown the strongest inhibitory activity followed by chloroform extract. Quercetin and phytol were associated with the methanolic and chloroform extracts which were identified using TLC and HPTLC techniques. During the thirty days of the study, the induction of diabetes in the rats exhibited persistent hyperglycemia, hyperlipidemia, higher escape latency during training trials and reduced time spent in target quadrant in probe trial in Morris water maze test, and increased escape latency in EPM task. Regimen of METD (200 and 400 mg/kg) in the diabetic rats reduced the glucose levels in blood, lipid, and liver profile and showed positive results on Morri’s water and elevated plus maze tasks. During the investigation, it was determined that Trianthema decandra extracts and the chemical constituent’s quercetin and phytol in it had anti-diabetic and neuroprotective activities.

Graphical Abstract

中文翻译：

---

富含黄酮类化合物 Trianthema decandra 可改善高血糖大鼠的认知功能障碍

摘要

本研究旨在评估Tianthema decandra（METD）甲醇提取物对大鼠高血糖相关认知障碍的神经保护能力。采用薄层色谱法和高效薄层色谱法对紫草提取物进行标准化。为了验证分离化合物的身份和纯度，使用各种技术（包括紫外-可见分光光度法、FT-IR、H-NMR 和质谱法）对它们进行分离和表征。在体外评估提取物的α-淀粉酶和α-葡萄糖苷酶抑制特性。利用 Morri 水 (MWM) 和高架十字迷宫 (EPM) 模型以及乙酰胆碱酯酶 (AChE) 活性筛选 METD 对高血糖大鼠的神经保护作用。天花提取物及其化学成分槲皮素和植醇对α-淀粉酶和α-葡萄糖苷酶等酶具有显着的保护作用。甲醇和水醇提取物显示出最强的抑制活性，其次是氯仿提取物。槲皮素和植醇与甲醇和氯仿提取物相关，并使用薄层色谱和高效薄层色谱技术进行鉴定。在为期三十天的研究中，诱导糖尿病的大鼠表现出持续的高血糖、高脂血症、训练试验中较高的逃避潜伏期以及莫里斯水迷宫试验中的探索试验中在目标象限中花费的时间减少，以及EPM任务中的逃避潜伏期增加。糖尿病大鼠的 METD 治疗方案（200 和 400 毫克/千克）降低了血液中的葡萄糖水平、血脂和肝脏特征，并且在莫里水和高架十字迷宫任务中显示出积极的结果。在研究过程中，确定了白千层提取物及其化学成分槲皮素和植醇具有抗糖尿病和神经保护活性。

图形概要