Genetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to further characterize risks to this important group of non-target organisms. In this work, exclusive feeding of frozen spider mites (Tetranychus urticae, Acari: Tetranychidae) from Bt cotton confirmed adverse effects on longevity and fecundity of O. majusculus adults. Alternate feeding of Bt protein-containing spider mites and Bt-free Ephestia kuehniella (Lepidoptera: Pyralidae) eggs mitigated effects on longevity, but not on fecundity. When living larvae of Spodoptera littoralis (Lepidoptera: Noctuidae) from Bt cotton were fed to the predators, however, no effects on longevity and reproduction of female O. majusculus were observed, despite the fact that Bt protein concentrations in larvae were almost as high as concentrations in spider mites. When a diverse mix of prey species with various Bt protein concentrations is consumed in the field, it is unlikely that exposure of Orius spp. to Mpp51Aa2 is high enough to exert adverse effects on predator populations. MON 88702 cotton may thus be a valuable tool for integrated management of sucking pests.

基因工程 (GE) 棉花事件 MON 88702，从苏云金芽孢杆菌(Bt) 中产生 Mpp51Aa2（以前称为 mCry51Aa2），可控制吸吮害虫，例如草盲蝽属 ( Lygus spp)。 （半翅目：Miridae）和蓟马（Thysanoptera）。摄入高剂量的杀虫蛋白会对有益的捕食性Orius属的生命表参数产生不利影响。 （半翅目：花蝽科）。这引发了实验室研究更现实的食物处理，包括含有和不含 Bt 蛋白的猎物类型的不同组合，以进一步表征这一重要非目标生物体的风险。在这项工作中，仅用 Bt 棉花喂养冷冻叶螨（Tetranychus urticae ，螨虫：Tetranychidae）证实了对O. majusculus成虫的寿命和繁殖力的不利影响。交替喂养含 Bt 蛋白的叶螨和不含 Bt 的Ephestia kuehniella（鳞翅目：螟科）卵可减轻对寿命的影响，但不会降低对繁殖力的影响。然而，当将来自 Bt 棉花的斜纹夜蛾（鳞翅目：夜蛾科）的活幼虫喂食给捕食者时，尽管幼虫中的 Bt 蛋白浓度几乎与红蜘蛛中的浓度。当在野外消耗具有不同 Bt 蛋白浓度的多种猎物物种时，Orius spp 的暴露不太可能。 Mpp51Aa2 的值足够高，足以对捕食者种群产生不利影响。因此，MON 88702 棉花可能是综合防治刺吸式害虫的宝贵工具。