In the present study, plasma activated water (PAW) immersion with different discharge times were applied to investigate their effects on broccoli seed germination, sprouts growth, antioxidant enzyme activities, polyphenol and anthocyanin content as well as sulforaphane formation. The seed germination rate, germination index, vigor index, water absorption, wettability and sprouts growth were promoted by PAW treatments, and plasma discharge for 30 min (PAW30) treatment showed the best germination rate and sprouts growth. No significant difference was found in color, polyphenol and anthocyanin content as well as myrosinase activity among different PAW treatments. The superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and glutathione peroxidase (GPX) enzyme activities of broccoli sprouts were enhanced significantly by all PAW treatments. Besides, PAW15, PAW30 and PAW60 treatments also increased significantly the sulforaphane formation of 5 d sprouts, which increased by 7.09%, 10.34% and 5.43% compared to the control, respectively. Therefore, these data indicated that seeds immersed with PAW30 could be used as a promising strategy to enhance the seed germination and quality of broccoli sprouts.

在本研究中，采用不同放电时间的等离子体活化水（PAW）浸泡来研究其对西兰花种子发芽、芽生长、抗氧化酶活性、多酚和花青素含量以及萝卜硫素形成的影响。 PAW处理对种子发芽率、发芽指数、活力指数、吸​​水性、润湿性和芽苗生长均有促进作用，其中等离子放电30 min（PAW30）处理的发芽率和芽苗生长效果最好。不同PAW处理之间颜色、多酚和花青素含量以及黑芥子酶活性没有发现显着差异。所有 PAW 处理均显着增强了西兰花芽的超氧化物歧化酶 (SOD)、过氧化物酶 (POD)、过氧化氢酶 (CAT) 和谷胱甘肽过氧化物酶 (GPX) 酶活性。此外，PAW15、PAW30和PAW60处理还显着增加了5 d芽的萝卜硫素形成量，分别比对照增加了7.09%、10.34%和5.43%。因此，这些数据表明，用 PAW30 浸泡种子可以作为提高西兰花芽的种子发芽和质量的有前景的策略。