Abstract

The work is devoted to the study of neurodegenerative changes in the ultrastructural organization of hippocampal CA1 in adult rats that have suffered prenatal hyperhomocysteinemia (pHHC). Electron microscopy in the neural networks of the CA1 region of the dorsal hippocampus in adult rats with pHHC, in contrast to control animals, revealed signs of pathological changes: degeneration of pyramidal neurons and destruction of the myelin sheath of axons, as well as destruction of the axial cylinders of basal and apical dendrites directed from a pyramidal layer of neurons in the direction of tractus temporammonic or Schaffer collaterals, respectively. In control animals, on the distal branches of dendrites in the layers of the stratum oriens and stratum radiatum, using the Golgi method, a dense network of varicose dendritic extensions was identified, providing an increase in the area of synaptic contacts. In rats that have undergone pHHC, significant destructive changes are found in these dendritic varicosities: destruction of mitochondrial cristae and the appearance of dilated cisterns. In adult rats with pHHC, it completely eliminates the preference for the smell of valerian, which is normally a physiologically significant stimulus, which indicates a negative effect of pHHC on the functioning of the olfactory analyzer, the activity of which is closely connected with the hippocampus. The obtained facts indicate the detrimental effect of homocysteine on the structure and interneuronal connections in the nervous tissue of the CA1 region of the dorsal hippocampus, as a morphological substrate for the integration of stimuli entering it.

中文翻译：

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产前高同型半胱氨酸血症成年大鼠背海马CA1区神经退行性改变的研究

摘要

这项工作致力于研究患有产前高同型半胱氨酸血症 (pHHC) 的成年大鼠海马 CA1 超微结构组织的神经退行性变化。与对照动物相比，患有 pHHC 的成年大鼠背侧海马 CA1 区神经网络的电子显微镜显示了病理变化的迹象：锥体神经元变性和轴突髓鞘破坏，以及基底树突和顶端树突的轴向圆柱体分别从神经元锥体层定向到颞束或谢弗络脉方向。在对照动物中，在东方层和放射层的树突远端分支上，使用高尔基方法，识别出静脉曲张树突延伸的致密网络，从而增加了突触接触面积。在经历 pHHC 的大鼠中，在这些树突静脉曲张中发现了显着的破坏性变化：线粒体嵴破坏和扩张池的出现。在患有 pHHC 的成年大鼠中，它完全消除了对缬草气味的偏好，缬草的气味通常是一种生理上显着的刺激，这表明 pHHC 对嗅觉分析器的功能产生负面影响，而嗅觉分析器的活动与海马体密切相关。所获得的事实表明，同型半胱氨酸对背侧海马 CA1 区神经组织的结构和神经元间连接产生有害影响，作为进入其中的刺激整合的形态学底物。