Through the integration of CRISPR/Cpf1 with optogenetics and a reduction-responsive motif, we have developed a photoactivatable cross-linked crRNA that enables precise genome editing upon light exposure. This system also allows for termination of editing activity through external application of reducing agent. The dual-stimuli-responsive CRISPR/Cpf1 editing process operates in a unique OFF → ON → OFF sequence, making it a valuable tool for investigating time-sensitive biological events.

中文翻译：

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通过交联 crRNA 的正交激活和失活对 CRISPR/Cpf1 编辑进行化学控制

通过将 CRISPR/Cpf1 与光遗传学和还原响应基序相结合，我们开发了一种可光激活的交联 crRNA，可在光照下实现精确的基因组编辑。该系统还允许通过外部应用还原剂来终止编辑活动。双刺激响应 CRISPR/Cpf1 编辑过程以独特的 OFF → ON → OFF 顺序运行，使其成为研究时间敏感的生物事件的宝贵工具。