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Celine, a long interspersed nuclear element retrotransposon, colonizes in the centromeres of poplar chromosomes Plant Physiol. (IF 7.4) Pub Date : 2024-04-23 Haoyang Xin, Yiduo Wang, Wenli Zhang, Bao Yu, Pavel Neumann, Yihang Ning, Tao Zhang, Yufeng Wu, Ning Jiang, Jiming Jiang, Mengli Xi
Centromeres in most multicellular eukaryotes are composed of long arrays of repetitive DNA sequences. Interestingly, several transposable elements, including the well-known long terminal repeat (LTR) retrotransposon CRM (centromeric retrotransposon of maize), were found to be enriched in functional centromeres marked by the centromeric histone H3 (CENH3). Here we report a centromeric long interspersed
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Co-repressors AtSDR4L and DIG1 interact with transcription factor VAL2 and promote Arabidopsis seed-to-seedling transition Plant Physiol. (IF 7.4) Pub Date : 2024-04-23 Bailan Lu, Milad Alizadeh, Ryan Hoy, Renwei Zheng, Dongeun Go, Liang Song
Two transcriptional co-repressors physically interact with a transcription factor that is known to recruit a multi-protein complex, which promotes the repression of seed maturation genes by depositing trimethylation marks on lysine 27 of the histone 3 tails.
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A group of L-type lectin receptor kinases function redundantly in mediating extracellular NAD(P) signaling in Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-04-23 Qi Li, Mingxi Zhou, Fiona Harris, Zhonglin Mou
An Arabidopsis mutant lacking several L-type lectin receptor kinases shows severely reduced NAD(P)-induced local and systemic immunity and biological induction of systemic acquired resistance.
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A tale of two pumps: Blue light and abscisic acid alter Arabidopsis leaf hydraulics via bundle sheath cell H + -ATPases Plant Physiol. (IF 7.4) Pub Date : 2024-04-23 Tanmayee Torne-Srivastava, Yael Grunwald, Ahan Dalal, Adi Yaaran, Menachem Moshelion, Nava Moran
The bundle sheath cell (BSC) layer tightly enveloping the xylem throughout the leaf is recognized as a major signal-perceiving “valve” in series with stomata, regulating leaf hydraulic conductance (Kleaf) and thereby radial water flow via the transpiring leaf. The BSC blue light (BL) signaling pathway increases Kleaf and the underlying BSC water permeability. Here, we explored the hypothesis that BSCs
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Imaging of labile Fe2+ and Fe3+ in living Arabidopsis thaliana roots Plant Physiol. (IF 7.4) Pub Date : 2024-04-22 Carine Alcon, Arnaud Comte, Catherine Curie, Tou Cheu Xiong
Adapting fluorescent iron imaging to living plants enables visualizing labile Fe2+ and Fe3+ pools, revealing the heterogeneous distribution of iron redox status at the tissue and cellular levels.
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Glutathionylation of a glycolytic enzyme promotes cell death and vigor loss during aging of elm seeds Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Ying Li, Yu Wang, Yu-Qi He, Tian-Tian Ye, Xu Huang, Hao Wu, Tian-Xiao Ma, Hugh W Pritchard, Xiao-Feng Wang, Hua Xue
Seed deterioration during storage is a major problem in agricultural and forestry production and for germplasm conservation. Our previous studies have shown that a mitochondrial outer membrane protein VOLTAGE-DEPENDENT ANION CHANNEL (VDAC) is involved in programmed cell death (PCD)-like viability loss during the controlled deterioration treatment (CDT) of elm (Ulmus pumila L.) seeds, but its underlying
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Jasmonate mimic modulates cell elongation by regulating antagonistic bHLH transcription factors via brassinosteroid signaling Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Xing Wang, Zhaobin Ren, Shipeng Xie, Zhaohu Li, Yuyi Zhou, Liusheng Duan
Lodging restricts growth, development, and yield formation in maize (Zea mays L.). Shorter internode length is beneficial for lodging tolerance. However, although brassinosteroids (BRs) and jasmonic acid (JA) are known to antagonistically regulate internode growth, the underlying molecular mechanism is still unclear. In this study, application of the JA mimic coronatine (COR) inhibited basal internode
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BRZ-INSENSITIVE-LONG HYPOCOTYL8 inhibits kinase-mediated phosphorylation to regulate brassinosteroid signaling Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Zhana Chagan, Genki Nakata, Shin Suzuki, Ayumi Yamagami, Ryo Tachibana, Surina Surina, Shozo Fujioka, Minami Matsui, Tetsuo Kushiro, Takuya Miyakawa, Tadao Asami, Takeshi Nakano
Glycogen synthase kinase 3 (GSK3) is an evolutionarily conserved serine/threonine protein kinase in eukaryotes. In plants, the GSK3-like kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2) functions as a central signaling node through which hormonal and environmental signals are integrated to regulate plant development and stress adaptation. BIN2 plays a major regulatory role in brassinosteroid (BR) signaling
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RNA splicing modulates the postharvest physiological deterioration of cassava storage root Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Jinbao Gu, Xiaowen Ma, Qiuxiang Ma, Zhiqiang Xia, Yan Lin, Jianbo Yuan, Yang Li, Cong Li, Yanhang Chen, Wenquan Wang, Peng Zhang, Zhen-Yu Wang
Rapid postharvest physiological deterioration (PPD) of cassava (Manihot esculenta Crantz) storage roots is a major constraint that limits the potential of this plant as a food and industrial crop. Extensive studies have been performed to explore the regulatory mechanisms underlying the PPD processes in cassava to understand their molecular and physiological responses. However, the exceptional functional
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Increased cytoplasmic Mg2+ level contributes to rice salicylic acid accumulation and broad-spectrum resistance Plant Physiol. (IF 7.4) Pub Date : 2024-04-18 Wenzhi Zeng, Yanjun Zhang, Xinyue Tian, Wenyan Li, Han Meng, Yuanchang Zhou, Zonghua Wang, Zhichang Chen, Kewei Zhang, Mo Wang
Elevating Mg2+ levels in rice cytoplasm by genetic modification or exogenous supply increases salicylic acid accumulation and enhances broad-spectrum resistance to pathogens.
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A pipeline for identification of causal mutations in barley identifies Xantha-j as the chlorophyll synthase gene Plant Physiol. (IF 7.4) Pub Date : 2024-04-17 David Stuart, Shakhira Zakhrabekova, Morten Egevang Jørgensen, Christoph Dockter, Mats Hansson
Thousands of barley (Hordeum vulgare L.) mutants have been isolated over the last century, and many are stored in gene banks across various countries. In the present work, we developed a pipeline to efficiently identify causal mutations in barley. The pipeline is also efficient for mutations located in centromeric regions. Through bulked-segregant analyses using whole genome sequencing of pooled F2
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3D reconstruction and multi-omics analysis reveal a unique pattern of embryogenesis in Ginkgo biloba Plant Physiol. (IF 7.4) Pub Date : 2024-04-17 Lingyu Ma, Zijian Hu, Weiwei Shen, Yingying Zhang, Guangchao Wang, Bang Chang, Jinkai Lu, Yaning Cui, Huimin Xu, Yun Feng, Biao Jin, Xi Zhang, Li Wang, Jinxing Lin
Ginkgo (Ginkgo biloba L.) is one of the earliest extant species in seed plant phylogeny. Embryo development patterns can provide fundamental evidence for the origin, evolution, and adaptation of seeds. However, the architectural and morphological dynamics during embryogenesis in Ginkgo biloba (G. biloba) remain elusive. Herein, we obtained over 2200 visual slices from three stages of embryo development
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BnaABF3 and BnaMYB44 regulate the transcription of zeaxanthin epoxidase genes in carotenoid and abscisic acid biosynthesis Plant Physiol. (IF 7.4) Pub Date : 2024-04-15 Shenhua Ye, Yingying Huang, Tiantian Ma, Xiaowei Ma, Rihui Li, Jinxiong Shen, Jing Wen
Zeaxanthin epoxidase (ZEP) is a key enzyme that catalyzes the conversion of zeaxanthin to violaxanthin in the carotenoid and abscisic acid (ABA) biosynthesis pathways. The rapeseed (Brassica napus) genome has 4 ZEP (BnaZEP) copies that are suspected to have undergone subfunctionalization, yet the 4 genes’ underlying regulatory mechanisms remain unknown. Here, we genetically confirmed the functional
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B-Box transcription factor BBX28 requires CONSTITUTIVE PHOTOMORPHOGENESIS1 to induce shade-avoidance response in Arabidopsis thaliana Plant Physiol. (IF 7.4) Pub Date : 2024-04-15 Maite Saura-Sánchez, Gabriel Gomez-Ocampo, Matías Ezequiel Pereyra, Carla Eliana Barraza, Andrés H Rossi, Juan P Córdoba, Javier Francisco Botto
Shade avoidance syndrome is an important adaptive strategy. Under shade, major transcriptional rearrangements underlie the reallocation of resources to elongate vegetative structures and redefine the plant architecture to compete for photosynthesis. BBX28 is a B-box transcription factor involved in seedling de-etiolation and flowering in Arabidopsis (Arabidopsis thaliana), but its function in shade-avoidance
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The trichome pattern diversity of Cardamine shares genetic mechanisms with Arabidopsis but differs in environmental drivers Plant Physiol. (IF 7.4) Pub Date : 2024-04-12 Alberto Fuster-Pons, Alba Murillo-Sánchez, Belén Méndez-Vigo, Arnald Marcer, Bjorn Pieper, Rafael Torres-Pérez, Juan Carlos Oliveros, Miltos Tsiantis, Francisco Xavier Picó, Carlos Alonso-Blanco
Natural variation in trichome pattern (amount and distribution) is prominent among populations of many angiosperms. However, the degree of parallelism in the genetic mechanisms underlying this diversity and its environmental drivers in different species remain unclear. To address these questions, we analyzed the genomic and environmental bases of leaf trichome pattern diversity in Cardamine hirsuta
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Transcription factor PgNAC72 activates DAMMARENEDIOL SYNTHASE expression to promote ginseng saponin biosynthesis Plant Physiol. (IF 7.4) Pub Date : 2024-04-12 Ting Jiang, Yue Zhang, Gege Zuo, Tiao Luo, Hui Wang, Ru Zhang, Zhiyong Luo
Ginsenosides, the primary bioactive constituents in ginseng (Panax ginseng), possess substantial pharmacological potential and are in high demand in the market. The plant hormone methyl jasmonate (MeJA) effectively elicits ginsenoside biosynthesis in P. ginseng, though the regulatory mechanism remains largely unexplored. NAC transcription factors are critical in intricate plant regulatory networks
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Direct and indirect responses of the Arabidopsis transcriptome to an induced increase in trehalose 6-phosphate Plant Physiol. (IF 7.4) Pub Date : 2024-04-09 Omri Avidan, Marina C M Martins, Regina Feil, Marc Lohse, Federico M Giorgi, Armin Schlereth, John E Lunn, Mark Stitt
Trehalose 6-phosphate (Tre6P) is an essential signal metabolite that regulates the level of sucrose, linking growth and development to the metabolic status. We hypothesized that Tre6P plays a role in mediating the regulation of gene expression by sucrose. To test this, we performed transcriptomic profiling on Arabidopsis (Arabidopsis thaliana) plants that expressed a bacterial TREHALOSE 6-PHOSPHATE
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Rice transcription factor OsWRKY37 positively regulates flowering time and grain fertility under copper deficiency Plant Physiol. (IF 7.4) Pub Date : 2024-04-09 Chenchen Ji, Haixing Li, Jingli Ding, Lu Yu, Cuncang Jiang, Chuang Wang, Sheliang Wang, Guangda Ding, Lei Shi, Fangsen Xu, Hongmei Cai
Efficient uptake, translocation, and distribution of Cu to rice (Oryza sativa) spikelets is crucial for flowering and yield production. However, the regulatory factors involved in this process remain unidentified. In this study, we isolated a WRKY transcription factor gene induced by Cu deficiency, OsWRKY37, and characterized its regulatory role in Cu uptake and transport in rice. OsWRKY37 was highly
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Multimodal imaging analysis in silver fir reveals coordination in cellulose and lignin deposition Plant Physiol. (IF 7.4) Pub Date : 2024-04-09 Gonzalo Pérez-de-Lis, Béatrice Richard, Fabienne Quilès, Aurélie Deveau, Ignatius-Kristia Adikurnia, Cyrille B K Rathgeber
Despite lignin being a key component of wood, the dynamics of tracheid lignification are generally overlooked in xylogenesis studies, which hampers our understanding of environmental drivers and blurs the interpretation of isotopic and anatomical signals stored in tree rings. Here, we analyzed cell wall formation in silver fir (Abies alba Mill.) tracheids to determine if cell wall lignification lags
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Photosynthetic capacity and assimilate transport of the lower canopy influence maize yield under high planting density Plant Physiol. (IF 7.4) Pub Date : 2024-04-09 Yanyan Yan, Fengying Duan, Xia Li, Rulang Zhao, Peng Hou, Ming Zhao, Shaokun Li, Yonghong Wang, Tingbo Dai, Wenbin Zhou
Photosynthesis is a major trait of interest for development of high-yield crop plants. However, little is known about the effects of high-density planting on photosynthetic responses at the whole-canopy level. Using the high-yielding maize (Zea mays L.) cultivars ‘LY66’, ‘MC670’, and ‘JK968’, we here conducted a two-year field experiment to assess ear development in addition to leaf characteristics
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Age-dependent changes in leaf size in apple are governed by a cytokinin-integrated module Plant Physiol. (IF 7.4) Pub Date : 2024-04-08 Xumei Jia, Shuo Xu, Yuting Wang, Lu Jin, Tengteng Gao, Zhijun Zhang, Chao Yang, Yubin Qing, Chao Li, Fengwang Ma
Plants undergo various age-dependent changes in leaf morphology during the transition from the juvenile to the adult stage. However, the precise molecular mechanisms governing these changes in apple (Malus domestica) remain unknown. Here, we showed that CYTOKININ OXIDASE/DEHYDROGENASE5 (MdCKX5), an age-dependent gene, encodes a functional CKX enzyme and serves as the common downstream target of SQUAMOSA
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Global dynamics and cytokinin participation of salt gland development trajectory in recretohalophyte Limonium bicolor Plant Physiol. (IF 7.4) Pub Date : 2024-04-08 Boqing Zhao, Yaru Gao, Qiuyu Ma, Xi Wang, Jian-Kang Zhu, Weiqiang Li, Baoshan Wang, Fang Yuan
Salt gland is an epidermal Na+ secretory structure that enhances salt resistance in the recretohalophyte sea lavender (Limonium bicolor). To elucidate the salt gland development trajectory and related molecular mechanisms, we performed single-cell RNA sequencing of L. bicolor protoplasts from young leaves at salt gland initiation and differentiation stages. Dimensionality reduction analyses defined
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The CELL NUMBER REGULATOR FW2.2 protein regulates cell-to-cell communication in tomato by modulating callose deposition at plasmodesmata Plant Physiol. (IF 7.4) Pub Date : 2024-04-08 Arthur Beauchet, Norbert Bollier, Magali Grison, Valérie Rofidal, Frédéric Gévaudant, Emmanuelle Bayer, Nathalie Gonzalez, Christian Chevalier
FW2.2 (standing for FRUIT WEIGHT 2.2), the founding member of the CELL NUMBER REGULATOR (CNR) gene family, was the first cloned gene underlying a quantitative trait locus (QTL) governing fruit size and weight in tomato (Solanum lycopersicum). However, despite this discovery over 20 years ago, the molecular mechanisms by which FW2.2 negatively regulates cell division during fruit growth remain undeciphered
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Nα-acetyltransferase NAA50 mediates plant immunity independent of the Nα-acetyltransferase A complex Plant Physiol. (IF 7.4) Pub Date : 2024-04-08 Laura Armbruster, Marlena Pożoga, Zhongshou Wu, Jürgen Eirich, Karen Thulasi Devendrakumar, Carolina De La Torre, Pavlina Miklánková, Monika Huber, Fabian Bradic, Gernot Poschet, Jonas Weidenhausen, Sabine Merker, Thomas Ruppert, Carsten Sticht, Irmgard Sinning, Iris Finkemeier, Xin Li, Rüdiger Hell, Markus Wirtz
In humans and plants, 40% of the proteome is co-translationally acetylated at the N-terminus by a single Nα-acetyltransferase (Nat) termed NatA. The core NatA complex is comprised of the catalytic subunit Nα- acetyltransferase 10 (NAA10) and the ribosome-anchoring subunit NAA15. The regulatory subunit Huntingtin Yeast Partner K (HYPK) and the acetyltransferase NAA50 join this complex in humans. Even
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LIKE EARLY STARVATION 1 interacts with amylopectin during starch biosynthesis Plant Physiol. (IF 7.4) Pub Date : 2024-04-04 Rayan Osman, Mélanie Bossu, David Dauvillée, Corentin Spriet, Chun Liu, Samuel C Zeeman, Christophe D’Hulst, Coralie Bompard
Starch is the major energy storage compound in plants. Both transient starch and long-lasting storage starch accumulate in the form of insoluble, partly crystalline granules. The structure of these granules is related to the structure of the branched polymer amylopectin: linear chains of glucose units organized in double helices that align to form semi-crystalline lamellae, with branch points located
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Naringenin restricts the colonization and growth of Ralstonia solanacearum in tobacco mutant KCB-1 Plant Physiol. (IF 7.4) Pub Date : 2024-04-04 Haoqi Shi, Jiale Jiang, Wen Yu, Yazhi Cheng, Shengxin Wu, Hao Zong, Xiaoqiang Wang, Anming Ding, Weifeng Wang, Yuhe Sun
Bacterial wilt severely jeopardizes plant growth and causes enormous economic loss in the production of many crops, including tobacco (Nicotiana tabacum). Here, we first demonstrated that the roots of bacterial wilt-resistant tobacco mutant KCB-1 can limit the growth and reproduction of Ralstonia solanacearum. Secondly, we demonstrated that KCB-1 specifically induced an upregulation of naringenin content
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Key transcription factors regulate fruit ripening and metabolite accumulation in tomato Plant Physiol. (IF 7.4) Pub Date : 2024-04-02 Huimin Jia, Yaping Xu, Yuanwen Deng, Yinhuan Xie, Zhongshan Gao, Zhaobo Lang, Qingfeng Niu
Fruit ripening is a complex process involving dynamic changes to metabolites and is controlled by multiple factors, including transcription factors (TFs). Several TFs are reportedly essential regulators of tomato (Solanum lycopersicum) fruit ripening. To evaluate the effects of specific TFs on metabolite accumulation during fruit ripening, we combined CRISPR/Cas9-mediated mutagenesis with metabolome
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Arabidopsis Fhit-like Tumor Suppressor Resumes Early Terminated constitutive ethylene response1-10 mRNA Translation Plant Physiol. (IF 7.4) Pub Date : 2024-04-02 Ranran Zhang, Wei Zhang, Chenrunshu Wang, Chi-Kuang Wen
The Arabidopsis (Arabidopsis thaliana) constitutive ethylene response1-10 (ctr1-10) mutant produces a reduced level of CTR1 protein and exhibits a weak ctr1 mutant phenotype. Sequence analysis revealed highly active translation of the upstream open reading frame (uORF) at the extended 5’-UTR of the ctr1-10 mRNA, resulting from T-DNA insertion. Enhancer screening for ctr1-10 isolated the fragile histidine
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Hypoxia in tomato (Solanum lycopersicum) fruit during ripening: biophysical elucidation by a 3D reaction-diffusion model Plant Physiol. (IF 7.4) Pub Date : 2024-03-28 Hui Xiao, Pieter Verboven, Shuai Tong, Ole Pedersen, Bart Nicolaï
Respiration provides energy, substrates and precursors to support physiological changes of the fruit during climacteric ripening. A key substrate of respiration is oxygen that needs to be supplied to the fruit in a passive way by gas transfer from the environment. Oxygen gradients may develop within the fruit due to its bulky size and the dense fruit tissues, potentially creating hypoxia that may have
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AGC kinases OXI1 and AGC2-2 regulate camalexin secretion and disease resistance by phosphorylating transporter PDR6 Plant Physiol. (IF 7.4) Pub Date : 2024-03-27 Juan Han, Chang-xin Liu, Jian Liu, Cheng-run Wang, Shun-chang Wang, Guopeng Miao
Plant transporters regulating the distribution of secondary metabolites play critical roles in defending against pathogens, insects, and interacting with beneficial microbes. The phosphorylation of these transporters can alter their activity, stability, and intracellular protein trafficking. However, the regulatory mechanism underlying this modification remains elusive. In this study, we discovered
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A wave of specific transcript and protein accumulation accompanies pollen dehydration Plant Physiol. (IF 7.4) Pub Date : 2024-03-27 Heven Sze, Božena Klodová, John M Ward, Jeffrey F Harper, Ravishankar Palanivelu, Mark A Johnson, David Honys
In flowering plants, male gametes are immotile and carried by dry pollen grains to the female organ. Dehydrated pollen is thought to withstand abiotic stress when grains are dispersed from the anther to the pistil, after which sperm cells are delivered via pollen tube growth for fertilization and seed set. Yet, the underlying molecular changes accompanying dehydration and the impact on pollen development
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Abscisic acid activates transcription factor module MdABI5–MdMYBS1 during carotenoid-derived apple fruit coloration Plant Physiol. (IF 7.4) Pub Date : 2024-03-27 Dongjie Jia, Yuchen Li, Kun Jia, Benchang Huang, Qingyuan Dang, Huimin Wang, Xinyuan Wang, Chunyu Li, Yugang Zhang, Jiyun Nie, Yongbing Yuan
Carotenoids are major pigments contributing to fruit coloration. We previously reported that the apple (Malus domestica Borkh.) mutant fruits of ‘Beni Shogun’ and ‘Yanfu 3’ show a marked difference in fruit coloration. However, the regulatory mechanism underlying this phenomenon remains unclear. In this study, we determined that carotenoid is the main factor influencing fruit flesh color. We identified
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Dynamic relationships among pathways producing hydrocarbons and fatty acids of maize silk cuticular waxes Plant Physiol. (IF 7.4) Pub Date : 2024-03-27 Keting Chen, Liza E Alexander, Umnia Mahgoub, Yozo Okazaki, Yasuhiro Higashi, Ann M Perera, Lucas J Showman, Derek Loneman, Tesia S Dennison, Miriam Lopez, Reid Claussen, Layton Peddicord, Kazuki Saito, Nick Lauter, Karin S Dorman, Basil J Nikolau, Marna D Yandeau-Nelson
The hydrophobic cuticle is the first line of defense between aerial portions of plants and the external environment. On maize (Zea mays L.) silks, the cuticular cutin matrix is infused with cuticular waxes, consisting of a homologous series of very long-chain fatty acids (VLCFAs), aldehydes, and hydrocarbons. Together with VLC fatty-acyl-CoAs (VLCFA-CoAs), these metabolites serve as precursors, intermediates
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Physical coupling of H3K4me3 demethylases and Polycomb repressive complex 2 to accelerate flowering in rice Plant Physiol. (IF 7.4) Pub Date : 2024-03-22 Hua Xuan, Nan Shi, Jianhao Chen, Yili Jiang, Hao Zhang, Chuanliang Chu, Shaoqing Li, Xiangsong Chen, Hongchun Yang
Two H3K4me3 demethylases physically interact with the Polycomb repressive complex 2, thereby altering methylation of a key flowering locus and promoting rice flowering.
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Chimeric mutations in grapevine ENHANCED DISEASE RESISTANCE1 improve resistance to powdery mildew without growth penalty Plant Physiol. (IF 7.4) Pub Date : 2024-03-20 Xue-Na Yu, Ye Guo, Qianling Yang, Haiyan Yu, Meng-Jiao Lu, Liang Zhao, Zhuo-Shuai Jin, Xiang-nan Xu, Jia-Yue Feng, Ying-Qiang Wen
Grapevine (Vitis vinifera L.) incurs severequality degradation and yield loss from powdery mildew, a major fungal disease caused by Erysiphe necator. ENHANCED DISEASE RESISTANCE1 (EDR1), a Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK), negatively regulates defense responses against powdery mildew in Arabidopsis (Arabidopsis thaliana). However, little is known about the role of the
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Epigenetic modification of a pectin methylesterase gene activates apoplastic iron reutilization in tomato roots Plant Physiol. (IF 7.4) Pub Date : 2024-03-20 Huihui Zhu, Jiayi Wang, Ru’nan Huang, Zheng’an Yang, Wei Fan, Li Huang, Jianli Yang, Weiwei Chen
Iron (Fe) distribution and reutilization are crucial for maintaining Fe homeostasis in plants. Here, we demonstrate that the tomato (Solanum lycopersicum) Colorless non-ripening (Cnr) epimutant exhibits increased Fe retention in cell wall pectin due to an increase in pectin methylesterase (PME) activity. This ultimately leads to Fe deficiency responses even under Fe-sufficient conditions when compared
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MORE FLORET1 controls anther development by negatively regulating key tapetal genes in both diploid and tetraploid rice Plant Physiol. (IF 7.4) Pub Date : 2024-03-20 Zijun Lu, Lianjun Zhu, Guobin Liang, Xiaoxia Li, Qihang Li, Yajing Li, Shengbo He, Jinwen Wu, Xiangdong Liu, Jingyi Zhang
Polyploid hybrid rice (Oryza sativa) has great potential for increasing yields. However, hybrid rice depends on male fertility and its regulation, which is less well studied in polyploid rice than in diploid rice. We previously identified a MYB transcription factor, MORE FLORET1 (MOF1), whose mutation causes male sterility in neo-tetraploid rice. MOF1 expression in anthers peaks at anther Stage 7 (S7)
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A Prenyltransferase Participates in the Biosynthesis of Anthraquinones in Rubia cordifolia Plant Physiol. (IF 7.4) Pub Date : 2024-03-19 Changzheng Liu, Ruishan Wang, Sheng Wang, Tong Chen, Chaogeng Lyu, Chuanzhi Kang, Xiufu Wan, Juan Guo, Qi Li, Luqi Huang, Lanping Guo
Anthraquinones constitute the largest group of natural quinones, which are used as safe natural dyes and have many pharmaceutical applications. In plants, anthraquinones are biosynthesized through two main routes: the polyketide pathway and the shikimate pathway. The latter primarily forms alizarin-type anthraquinones, and the prenylation of 1,4-dihydroxy-2-naphthoic acid is the first pathway-specific
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Acetylation of transcription factor BpTCP20 by acetyltransferase BpPDCE23 modulates salt tolerance in birch Plant Physiol. (IF 7.4) Pub Date : 2024-03-19 Zhujun Liu, Xinxin Shi, Zhibo Wang, Ming Qu, Caiqiu Gao, Chao Wang, Yucheng Wang
Teosinte branched 1/Cycloidea/Proliferating cell factor (TCP) transcription factors function in abiotic stress responses. However, how TCPs confer salt tolerance is unclear. Here, we characterized a TCP transcription factor, BpTCP20, that responds to salt stress in birch (Betula platyphylla Suk). Plants overexpressing BpTCP20 displayed increased salt tolerance, and Bptcp20 knockout mutants displayed
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KINASE-INDUCIBLE DOMAIN INTERACTING 8 regulates helical pod morphology in Medicago truncatula Plant Physiol. (IF 7.4) Pub Date : 2024-03-19 Qianxia Yu, Huan Du, Yuanyuan Huang, Xiao Lei, Xueting Wu, Jiayu Jiang, Wei Huang, Liangfa Ge
Leguminosae exhibits a wide diversity of legume forms with varying degrees of spiral morphologies, serving as an ideal clade for studying the growth and development of spiral organs. While soybean (Glycine max) develops straight pods, the pod of the model legume Medicago truncatula is a helix structure. Despite the fascinating structures and intensive description of the pods in legumes, little is known
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Debottlenecking the DOPA 4,5-dioxygenase step with enhanced tyrosine supply boosts betalain production in Nicotiana benthamiana Plant Physiol. (IF 7.4) Pub Date : 2024-03-18 Soyoung Jung, Hiroshi A Maeda
Synthetic biology provides emerging tools to produce valuable compounds in plant hosts as sustainable chemical production platforms. However, little is known about how supply and utilization of precursors is coordinated at the interface of plant primary and specialized metabolism, limiting our ability to efficiently produce high levels of target specialized metabolites in plants. L-Tyrosine is an aromatic
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E3 SUMO ligase SIZ1 splicing variants localize and function according to external conditions Plant Physiol. (IF 7.4) Pub Date : 2024-03-18 Jun Soo Kwak, Jong Tae Song, Hak Soo Seo
SIZ1 (SAP and MIZ1) is a member of the Siz/PIAS-type RING family of E3 SUMO (small ubiquitin-related modifier) ligases that play key roles in growth, development, and stress responses in plant and animal systems. Nevertheless, splicing variants of SIZ1 have not yet been characterized. Here, we identified four splicing variants of Arabidopsis (Arabidopsis thaliana) SIZ1, which encode three different
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GLABRA 2 regulates ETHYLENE OVERPRODUCER 1 accumulation during nutrient deficiency-induced root hair growth Plant Physiol. (IF 7.4) Pub Date : 2024-03-18 Jianing Cheng, Jinshu Wang, Shuangtian Bi, Mingyang Li, Lina Wang, Lu Wang, Tong Li, Xiaolan Zhang, Yue Gao, Lei Zhu, Che Wang
Root hairs (RHs), extensive structures of root epidermal cells, are important for plant nutrient acquisition, soil anchorage, and environmental interactions. Excessive production of the phytohormone ethylene (ET) leads to substantial root hair growth, manifested as tolerance to plant nutrient deficiencies. However, the molecular basis of ET production during root hair growth in response to nutrient
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MutL homolog 1 participates in interference-sensitive meiotic crossover formation in soybean Plant Physiol. (IF 7.4) Pub Date : 2024-03-16 Tao Wu, Suxin Yang, Junling Fang, Yongheng Ye, Yaohua Zhang, Jinshan Gao, Jiantian Leng, Zhirui Zhang, Kuanqiang Tang, Javaid Akhter Bhat, Xianzhong Feng
MutL homolog 1 (MLH1), a member of the MutL-homolog family, is required for normal recombination in most organisms. However, its role in soybean (Glycine max) remains unclear to date. Here, we characterized the Glycine max female and male sterility 1 (Gmfms1) mutation that reduces pollen grain viability and increases embryo sac abortion in soybean. Map-based cloning revealed that the causal gene of
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JASMONATE ZIM-DOMAIN PROTEIN 3 regulates photo- and thermo-morphogenesis through inhibiting PIF4 in Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-03-15 Junling Huai, Nan Gao, Yuanyuan Yao, Yanxin Du, Qiang Guo, Rongcheng Lin
Light and temperature are two major environmental factors that affect growth and development of plants during their life cycle. Plants have evolved complex mechanisms to adapt to varying external environments. Here, we show that JASMONATE ZIM-domain protein 3 (JAZ3), a jasmonic acid signaling component, acts as a factor to integrate light and temperature in regulating seedling morphogenesis. JAZ3 overexpression
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TCP transcription factor StAST1 represses potato tuberization by regulating tuberigen complex activity Plant Physiol. (IF 7.4) Pub Date : 2024-03-15 Xiaomeng Sun, Enshuang Wang, Liu Yu, Shengxuan Liu, Tiantian Liu, Jun Qin, Peng Jiang, Shuangshuang He, Xingkui Cai, Shenglin Jing, Botao Song
Potato (Solanum tuberosum L.) is cultivated worldwide for its underground tubers, which provide an important part of human nutrition and serve as a model system for belowground storage organ formation. Similar to flowering, stolon-expressed FLOWERING LOCUS T-like (FT-like) protein SELF-PRUNING 6A (StSP6A) plays an instrumental role in tuberization by binding to the bZIP transcription factors StABI5-like
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Deciphering Recursive Polyploidization in Lamiales and Reconstructing Their Chromosome Evolutionary Trajectories Plant Physiol. (IF 7.4) Pub Date : 2024-03-14 Jiangli Wang, Bowen Song, Minran Yang, Fubo Hu, Huilong Qi, Huizhe Zhang, Yuelong Jia, Yingjie Li, Zhenyi Wang, Xiyin Wang
Lamiales is an order of core eudicots with abundant diversity, and many Lamiales plants have important medicinal and ornamental values. Here, we comparatively reanalyzed 11 Lamiales species with well-assembled genome sequences and found evidence that Lamiales plants, in addition to a hexaploidization or whole-genome triplication (WGT) shared by core eudicots, experienced further polyploidization events
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Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Minori Nigishi, Ginga Shimakawa, Kansei Yamagishi, Ryosuke Amano, Shun Ito, Yoshinori Tsuji, Chikako Nagasato, Yusuke Matsuda
Anion transporters sustain a variety of physiological states in cells. Bestrophins belong to a Cl− and/or HCO3− transporter family conserved in bacteria, animals, algae, and plants. Recently, putative bestrophins were found in the green alga Chlamydomonas reinhardtii, where they are up-regulated under low CO2 conditions and play an essential role in the CO2-concentrating mechanism (CCM). The putative
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A nuclease-dead Cas9-derived tool represses target gene expression Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Bowen Wang, Xiaolin Liu, Zhenxiang Li, Kang Zeng, Jiangyi Guo, Tongxu Xin, Zhen Zhang, Jian-Feng Li, Xueyong Yang
Manipulation of gene expression is central to understanding gene function, engineering cell behavior, and altering biological traits according to production demands. Nuclease-dead Cas9 (dCas9), a variant of active Cas9, offers a versatile platform for the precise control of genome function without DNA cleavage. Notably, however, an effective and universal dCas9-based transcriptional repression system
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Subtilisin-like proteases from Fusarium graminearum induce plant cell death and contribute to virulence Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Jiang Xiong, Mingyu Luo, Yunshen Chen, Qianyong Hu, Ying Fang, Tongjun Sun, Guanjing Hu, Cui-Jun Zhang
Fusarium head blight (FHB), caused by Fusarium graminearum, causes huge annual economic losses in cereal production. To successfully colonize host plants, pathogens secrete hundreds of effectors that interfere with plant immunity and facilitate infection. However, the roles of most secreted effectors of F. graminearum in pathogenesis remain unclear. We analyzed the secreted proteins of F. graminearum
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A nanopore-based cucumber genome assembly reveals structural variations at two QTLs controlling hypocotyl elongation Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Bin Liu, Cheng-Cheng Shen, Shi-Wei Xia, Shan-Shan Song, Li-Hong Su, Yu Li, Qian Hao, Yan-Jun Liu, Dai-Lu Guan, Ning Wang, Wen-Jiao Wang, Xiang Zhao, Huan-Xiu Li, Xi-Xiang Li, Yun-Song Lai
The Xishuangbanna (XIS) cucumber (Cucumis sativus var. xishuangbannanesis) is a semiwild variety that has many distinct agronomic traits. Here, long -reads generated by Nanopore sequencing technology helped assembling a high-quality genome (contig N50 = 8.7 Mb) of landrace XIS49. A total of 10,036 structural/sequence variations (SVs) were identified when comparing with Chinese Long (CL), and known
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Metabolic crosstalk between hydroxylated monoterpenes and salicylic acid in tomato defense response against bacteria Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Julia Pérez-Pérez, Samuel Minguillón, Elías Kabbas-Piñango, Celia Payá, Laura Campos, Manuel Rodríguez-Concepción, Ana Espinosa-Ruiz, Ismael Rodrigo, José María Bellés, María Pilar López-Gresa, Purificación Lisón
Hydroxylated monoterpenes (HMTPs) are differentially emitted by tomato (Solanum lycopersicum) plants resisting bacterial infection. We have studied the defensive role of these volatiles in the tomato response to bacteria, whose main entrance are through stomatal apertures. Treatments with some HMTPs resulted in stomatal closure and pathogenesis-related protein 1 (PR1) induction. Particularly, α-terpineol
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Genetic engineering of drought and salt tolerant tomato via S-nitrosylated Δ1-pyrroline-5-carboxylate reductase Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Wei Liu, Jin-Wei Wei, Qing Shan, Minghui Liu, Jinghao Xu, Biao Gong
Drought and soil salinization substantially impact agriculture. While proline's role in enhancing stress tolerance is known, the exact molecular mechanism by which plants process stress signals and control proline synthesis under stress is still not fully understood. In tomato (Solanum lycopersicum L.), drought and salt stress stimulate nitric oxide (NO) production, which boosts proline synthesis by
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Chromosome ends initiate homologous chromosome pairing during rice meiosis Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Hanli You, Ding Tang, Huixin Liu, Yue Zhou, Yafei Li, Yi Shen, Zhiyun Gong, Hengxiu Yu, Minghong Gu, Jiming Jiang, Tao Zhang, Zhukuan Cheng
During meiotic prophase I, chromosomes undergo large-scale dynamics to allow homologous chromosome pairing, prior to which chromosome ends attach to the inner nuclear envelope and form a chromosomal bouquet. Chromosome pairing is crucial for homologous recombination and accurate chromosome segregation during meiosis. However, the specific mechanism by which homologous chromosomes recognize each other
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Peroxisomal 4-coumaroyl-CoA ligases participate in shikonin production in Lithospermum erythrorhizon Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Kohei Nakanishi, Hao Li, Takuji Ichino, Kanade Tatsumi, Keishi Osakabe, Bunta Watanabe, Koichiro Shimomura, Kazufumi Yazaki
4-Coumaroyl-CoA ligase (4CL) is a key enzyme in the phenylpropanoid pathway, which is involved in the biosynthesis of various specialized metabolites such as flavonoids, coumarins, lignans, and lignin. Plants have several 4CLs showing divergence in sequence: class I 4CLs involved in lignin metabolism, class II 4CLs associated with flavonoid metabolism, and atypical 4CLs and 4CL-like proteins of unknown
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Transcription factor OsWRKY72 controls rice leaf angle by regulating LAZY1-mediated shoot gravitropism Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Lei Liu, Lirong Zhao, Yunwei Liu, Yi Zhu, Shidie Chen, Lu Yang, Xia Li, Wanqin Chen, Zhiyu Xu, Peng Xu, Houping Wang, Diqiu Yu
Leaf angle is a major trait of ideal architecture, which is considered to influence rice (Oryza sativa) cultivation and grain yield. Although a few mutants with altered rice leaf inclination angle have been reported, the underlying molecular mechanism remains unclear. In this study, we showed that a WRKY transcription factor gene, OsWRKY72, was highly expressed in the leaf sheath and lamina joint.
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ABSCISIC ACID-INSENSITIVE 5-KIP-RELATED PROTEIN 1-SHOOT MERISTEMLESS modulates reproductive development of Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Wan-Ni Wang, Yu-Ting Wei, Sheng-Ting Zhao, Fu-Huan Yu, Jing-wen Wang, Cheng-yue Gu, Xin-Ran Liu, Na Sai, Jin-Lei Zhu, Qi-Meng Wang, Qin-Xin Bao, Xin-Rong Mu, Yu-xin Liu, Gary J Loake, Ji-hong Jiang, Lai-Sheng Meng
Soil (or plant) water deficit accelerates plant reproduction. However, the underpinning molecular mechanisms remain unknown. By modulating cell division/number, ABSCISIC ACID-INSENSITIVE 5 (ABI5), a key bZIP (basic (region) leucine zippers) transcription factor, regulates both seed development and abiotic stress responses. The KRP (KIP-RELATED PROTEIN) cyclin-dependent kinases (CDKs) play an essential
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Glutamine induces lateral root initiation, stress responses, and disease resistance in Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Hong-Sheng Liao, Kim-Teng Lee, Yi-Hsin Chung, Soon-Ziet Chen, Yi-Jie Hung, Ming-Hsiun Hsieh
The production of glutamine (Gln) from NO3- and NH4+ requires ATP, reducing power, and carbon skeletons. Plants may redirect these resources to other physiological processes using Gln directly. However, feeding Gln as the sole nitrogen (N) source has complex effects on plants. Under optimal concentrations, Arabidopsis (Arabidopsis thaliana) seedlings grown on Gln have similar primary root lengths,
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ROP GTPases with a geranylgeranylation motif modulate alkaloid biosynthesis in Catharanthus roseus Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Dikki Pedenla Bomzan, Anuj Sharma, Pamela Lemos Cruz, Ines Carqueijeiro, Léo Bellenger, Avanish Rai, Akshay Kumar Thippesh, Venkatesha S Chinnegowda, Durgesh Parihar, Eric Ducos, Vincent Courdavault, Dinesh A Nagegowda
Rho of Plant (ROP) GTPases function as molecular switches that control signaling processes essential for growth, development, and defense. However, their role in specialized metabolism is poorly understood. Previously, we demonstrated that inhibition of protein geranylgeranyl transferase (PGGT-I) negatively impacts the biosynthesis of monoterpenoid indole alkaloids (MIA) in Madagascar periwinkle (Catharanthus
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MicroRNA1432 regulates rice drought stress tolerance by targeting the CALMODULIN-LIKE2 gene Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Guangyu Luo, Lin Li, Xiaoyu Yang, Yu Yu, Lei Gao, Beixin Mo, Xuemei Chen, Lin Liu
Due to climate change, drought has become a major threat to rice (Oryza sativa L.) growth and yield worldwide. Understanding the genetic basis of drought tolerance in rice is therefore of great importance. Here, we identified a microRNA, miR1432, which regulates rice drought tolerance by targeting the CALMODULIN-LIKE2 (OsCaML2) gene. Mutation of MIR1432 (Bhardwaj et al., 2014). Moreover, recent studies