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Analysis and benchmarking of small and large genomic variants across tandem repeats Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-26 Adam C. English, Egor Dolzhenko, Helyaneh Ziaei Jam, Sean K. McKenzie, Nathan D. Olson, Wouter De Coster, Jonghun Park, Bida Gu, Justin Wagner, Michael A. Eberle, Melissa Gymrek, Mark J. P. Chaisson, Justin M. Zook, Fritz J. Sedlazeck
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Super7 passaging method to improve Chinese hamster ovary cell fed‐batch performance Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-25 Matthew J. Moran, Jin Chen, James M. Piret, R. Robert Balcarcel
Chinese hamster ovary (CHO) cells are widely used to manufacture biopharmaceuticals, most of all monoclonal antibodies (mAbs). Some CHO cell lines exhibit production instability, where the productivity of the cells decreases as a function of time in culture. To counter this, we designed a passaging strategy that, rather than maximizing the time spent in log‐growth phase, mimics the first 7 days of
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Integration of just‐in‐time learning with variational autoencoder for cell culture process monitoring based on Raman spectroscopy Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-24 Mohammad Rashedi, Hamid Khodabandehlou, Tony Wang, Matthew Demers, Aditya Tulsyan, Christopher Garvin, Cenk Undey
Protein production in the biopharmaceutical industry necessitates the utilization of multiple analytical techniques and control methodologies to ensure both safety and consistency. To facilitate real‐time monitoring and control of cell culture processes, Raman spectroscopy has emerged as a versatile analytical technology. This technique, categorized as a Process Analytical Technology, employs chemometric
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Assessing the laboratory performance of AI-generated enzymes Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-23
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Discovery of tumor-reactive T cell receptors by massively parallel library synthesis and screening Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-23 Ziva Moravec, Yue Zhao, Rhianne Voogd, Danielle R. Cook, Seon Kinrot, Benjamin Capra, Haiyan Yang, Brenda Raud, Jiayu Ou, Jiekun Xuan, Teng Wei, Lili Ren, Dandan Hu, Jun Wang, John B.A.G. Haanen, Ton N. Schumacher, Xi Chen, Ely Porter, Wouter Scheper
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Therapeutic application of circular RNA aptamers in a mouse model of psoriasis Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-23 Si-Kun Guo, Chu-Xiao Liu, Yi-Feng Xu, Xiao Wang, Fang Nan, Youkui Huang, Siqi Li, Shan Nan, Ling Li, Edo Kon, Chen Li, Meng-Yuan Wei, Rina Su, Jia Wei, Shiguang Peng, Nitay Ad-El, Jiaquan Liu, Dan Peer, Ting Chen, Li Yang, Ling-Ling Chen
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Computational scoring and experimental evaluation of enzymes generated by neural networks Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-23 Sean R. Johnson, Xiaozhi Fu, Sandra Viknander, Clara Goldin, Sarah Monaco, Aleksej Zelezniak, Kevin K. Yang
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The myriad ways to engineer cells Nat. Biomed. Eng. (IF 28.1) Pub Date : 2024-04-23
The widening range of strategies to alter the phenotypes and functions of mammalian cells is a boon for their biomedical applications.
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Stabilization of galactose oxidase by high hydrostatic pressure: Insights on the role of cavities size Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-23 Min J. Kang, José I. Reyes‐De‐Corcuera
High hydrostatic pressure stabilized galactose oxidase (GaOx) at 70.0–80.0°C against thermal inactivation. The pseudo‐first‐order rate constant of inactivation kinact decreased by a factor of 8 at 80°C and by a factor of 44 at 72.5°C. The most pronounced effect of pressure was at the lowest studied temperature of 70.0°C with an activation volume of inactivation ΔV‡ of 78.8 cm3 mol−1. The optimal pressure
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Why Japan lacks a vibrant biotech industry Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-22 Mark Kessel, Chris Vickrey
Recent analysis of the biopharmaceutical industry in Japan has emphasized that the lack of a thriving biotech ecosystem in that country is largely due to tight controls on drug pricing1. However, this is only one part of the explanation, and any strategy to promote Japanese biotech must acknowledge the full complexity of the problem. Japan has long punched above its weight in innovative research in
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Biobased short chain fatty acid production - Exploring microbial community dynamics and metabolic networks through kinetic and microbial modeling approaches Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-22 Merve Atasoy, William T. Scott Jr, Alberte Regueira, Miguel Mauricio-Iglesias, Peter J. Schaap, Hauke Smidt
In recent years, there has been growing interest in harnessing anaerobic digestion technology for resource recovery from waste streams. This approach has evolved beyond its traditional role in energy generation to encompass the production of valuable carboxylic acids, especially volatile fatty acids (VFAs) like acetic acid, propionic acid, and butyric acid. VFAs hold great potential for various industries
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Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells Nat. Biomed. Eng. (IF 28.1) Pub Date : 2024-04-19 DaeYong Lee, Kristin Huntoon, Yifan Wang, Minjeong Kang, Yifei Lu, Seong Dong Jeong, Todd M. Link, Thomas D. Gallup, Yaqing Qie, Xuefeng Li, Shiyan Dong, Benjamin R. Schrank, Adam J. Grippin, Abin Antony, JongHoon Ha, Mengyu Chang, Yi An, Liang Wang, Dadi Jiang, Jing Li, Albert C. Koong, John A. Tainer, Wen Jiang, Betty Y. S. Kim
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Optimization strategies for CO2 biological fixation Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-19 Xiutao Liu, Linqing Li, Guang Zhao, Peng Xiong
Global sustainable development faces a significant challenge in effectively utilizing CO. Meanwhile, CO biological fixation offers a promising solution. CO has the highest oxidation state (+4 valence state), whereas typical multi‑carbon chemicals have lower valence states. The Gibbs free energy (Δ) changes of CO reductive reactions are generally positive and this renders it necessary to input different
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Development of generic metabolic Raman calibration models using solution titration in aqueous phase and data augmentation for in‐line cell culture analysis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-19 Zhijun Zhang, Zhe Lang, Gong Chen, Hang Zhou, Weichang Zhou
This study presents a novel approach for developing generic metabolic Raman calibration models for in‐line cell culture analysis using glucose and lactate stock solution titration in an aqueous phase and data augmentation techniques. First, a successful set‐up of the titration method was achieved by adding glucose or lactate solution at several different constant rates into the aqueous phase of a bench‐top
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Engineering of the Lrp/AsnC‐type transcriptional regulator DecR as a genetically encoded biosensor for multilevel optimization of L‐cysteine biosynthesis pathway in Escherichia coli Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-18 Zhiyou Zhou, Zonglin Li, Yahui Zhong, Shuai Xu, Zhimin Li
L‐cysteine is an important sulfur‐containing amino acid being difficult to produce by microbial fermentation. Due to the lack of high‐throughput screening methods, existing genetically engineered bacteria have been developed by simply optimizing the expression of L‐cysteine‐related genes one by one. To overcome this limitation, in this study, a biosensor‐based approach for multilevel biosynthetic pathway
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Customized molecular glue complexes with desired properties Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Iris Marchal
Degron tags can trigger rapid and temporally controlled degradation of proteins using small molecules known as molecular glues. These molecular glues induce or stabilize protein interactions between a target protein and a ubiquitin ligase. As research tools, molecular glue complexes are limited by the large size of degron tags, which prevents integration in endogenous protein-coding genes. In a study
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World’s priciest drug treats MLD Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
The US Food and Drug Administration has approved the first therapy for metachromatic leukodystrophy (MLD), a rare fatal genetic disorder. The lysosomal storage disease affects about 40 children each year in the USA. It is caused by a mutation in the gene encoding the arylsulfatase enzyme that leads to progressive demyelination and progressive loss of motor and cognitive functions. There were previously
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Saving Cavendish Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Farmers have a green light to grow the first genetically modified banana. The wilt-proof strain of the Cavendish banana developed by researchers from the Queensland University of Technology is resistant to Panama disease (Fusarium wilt), a devastating fungus. The Office of the Gene Technology Regulator in Australia gave the go-ahead on 12 February to allow the genetically modified banana to be grown
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Prime editing Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Recent patents relating to methods and compositions for prime editing.
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FDA approves first MASH drug Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
The first drug to treat fatty liver disease due to metabolic dysfunction-associated steatohepatitis (MASH) has been given a green light by the US Food and Drug Administration. Madrigal Pharmaceuticals’ Rezdiffra (resmetirom) received an accelerated approval to treat the disease, previously known as non-alcoholic steatohepatitis (NASH). In this progressive liver condition fat buildup triggers inflammation
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Biotech news from around the world Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
The Ministry of Health and Welfare selects Johnson & Johnson’s JLABS to operate the country’s global accelerator platform. JLABS will engage with various local incubators and collaborators in the startup ecosystem to offer venture development programs, stimulate employment and encourage commercialization to enhance the global competitiveness of Korea’s life sciences sector. Costa Rica revises its biotech
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First gene-edited pig kidney transplant Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Surgeons at Massachusetts General Hospital have transplanted a pig kidney into a living person for the first time. On 16 March, a 62-year old man with end-stage kidney disease received a kidney from a genome-edited pig developed by eGenesis. The humanized pig organ was taken from a genetically engineered Yucatan miniature pig carrying a total of 69 gene edits designed to increase compatibility between
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RoseTTAFold expands to all-atom for biomolecular prediction and design Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Iris Marchal
Deep learning methods enable the structural prediction of proteins with high accuracy but are unable to model non-protein molecules that are essential for a protein’s biological function. Writing in Science, Krishna et al. introduce RoseTTAFold All-Atom (RFAA) to model the structure of full biological assemblies containing proteins, nucleic acids, small molecules, metals and covalent modifications
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Mapping the landscape of host–microbiome interactions Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Iris Marchal
The human body hosts countless microorganisms that play diverse roles in health and disease. However, the lack of tools capable of investigating these host–microbiota interactions at large scale has left many of them undiscovered. Writing in Nature, Sonnert et al. developed and validated a tool, named BASEHIT, to map the broad interplay between bacteria and human proteins. BASEHIT identified an extensive
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Prime editing deal flurry to nail down patent rights Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
The prime editing field is booming, with companies making strategic decisions to avoid an IP showdown.
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Startups probe hidden viruses in the ‘dark genome’ to treat disease Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Drug hunters are finding that ancient virus-like artifacts in the human genome could offer new avenues to treat neurodegeneration, cancer, autoimmunity and even aging with antibodies, vaccines and antiretroviral agents.
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Video game unleashes millions of citizen scientists on microbiome research Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
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Five questions with César de la Fuente Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Michael Francisco
A pioneer in the emerging fields of AI for antibiotic discovery and molecular de-extinction describes his transdisciplinary background and lifelong passion to understand biology.
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People Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Recent moves of note in and around the biotech and pharma industries.
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Mapping the global landscape for induced pluripotent stem cells from patents and clinical trials Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Liyang Lyu, Ye Feng, Borong Huang, Ren-He Xu, Yuanjia Hu
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Sharing best practices for educational programs on venture creation and commercialization Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Jordan Eidlisz, Zachary Hill-Whilton, Gabriel Vizgan, Daniel Cobos, Sadhana Chitale, Colleen Gillespie, Nabil Dib, Gabrielle Gold-von Simson
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Efficient production of l‐glutathione by whole‐cell catalysis with ATP regeneration from adenosine Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-17 Siqi Zuo, Jiarun Zhao, Pengfei Zhang, Wenqian Liu, Ke Bi, Peilian Wei, Jiazhang Lian, Zhinan Xu
l‐glutathione (GSH) is an important tripeptide compound with extensive applications in medicine, food additives, and cosmetics industries. In this work, an innovative whole‐cell catalytic strategy was developed to enhance GSH production by combining metabolic engineering of GSH biosynthetic pathways with an adenosine‐based adenosine triphosphate (ATP) regeneration system in Escherichia coli. Concretely
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Improving microbial phylogeny with citizen science within a mass-market video game Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-15 Roman Sarrazin-Gendron, Parham Ghasemloo Gheidari, Alexander Butyaev, Timothy Keding, Eddie Cai, Jiayue Zheng, Renata Mutalova, Julien Mounthanyvong, Yuxue Zhu, Elena Nazarova, Chrisostomos Drogaris, Kornél Erhart, Amélie Brouillette, Gabriel Richard, Randy Pitchford, Sébastien Caisse, Mathieu Blanchette, Daniel McDonald, Rob Knight, Attila Szantner, Jérôme Waldispühl
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Serial adaptive laboratory evolution enhances mixed carbon metabolic capacity of Escherichia coli Metab. Eng. (IF 8.4) Pub Date : 2024-04-16 Kangsan Kim, Donghui Choe, Minjeong Kang, Sang-Hyeok Cho, Suhyung Cho, Ki Jun Jeong, Bernhard Palsson, Byung-Kwan Cho
Microbes have inherent capacities for utilizing various carbon sources, however they often exhibit sub-par fitness due to low metabolic efficiency. To test whether a bacterial strain can optimally utilize multiple carbon sources, was serially evolved in L-lactate and glycerol. This yielded two end-point strains that evolved first in L-lactate then in glycerol, and vice versa. The end-point strains
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Engineering a solar formic acid/pentose (SFAP) pathway in Escherichia coli for lactic acid production Metab. Eng. (IF 8.4) Pub Date : 2024-04-15 Yajing Zhang, Tao Sun, Linqi Liu, Xupeng Cao, Weiwen Zhang, Wangyin Wang, Can Li
Microbial CO fixation into lactic acid (LA) is an important approach for low-carbon biomanufacturing. Engineering microbes to utilize CO and sugar as co-substrates can create efficient pathways through input of moderate reducing power to drive CO fixation into product. However, to achieve complete conservation of organic carbon, how to engineer the CO-fixing modules compatible with native central metabolism
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Biotechnology and Bioengineering: Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13
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Multivariate data analysis on multisensor measurement for inline process monitoring of adenovirus production in HEK293 cells Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Xingge Xu, Omar Farnós, Barbara C. M. F. Paes, Sean Nesdoly, Amine A. Kamen
In the era of Biopharma 4.0, process digitalization fundamentally requires accurate and timely monitoring of critical process parameters (CPPs) and quality attributes. Bioreactor systems are equipped with a variety of sensors to ensure process robustness and product quality. However, during the biphasic production of viral vectors or replication‐competent viruses for gene and cell therapies and vaccination
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Cover Image, Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Klemen Božič, Ajda Sedlar, Špela Kralj, Urh Černigoj, Aleš Štrancar, Rok Sekirnik
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Back Cover Image, Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Yixiao Zhang, Sri Madabhushi, Tiffany Tang, Hassan Raza, David J. Busch, Xi Zhao, James Ormes, Sen Xu, Joseph Moroney, Rubin Jiang, Henry Lin, Ren Liu
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Inferring gene regulatory networks from single-cell multiome data using atlas-scale external data Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-12 Qiuyue Yuan, Zhana Duren
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The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-12 Ruchika, Nabab Khan, Shagun Sanjivv Dogra, Ankit Saneja
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Semiautomated design and soluble expression of a chimeric antigen TbpAB01 from Glaesserella parasuis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-10 Jin‐Ping Chen, Lin Zhou, Jin‐Song Gong, Nan‐Kai Wang, Fen‐Fang Miao, Chang Su, Xin‐Le Gao, Guo‐Qiang Xu, Jin‐Song Shi, Zheng‐Hong Xu
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Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-08 Fabian P. Suchy, Daiki Karigane, Yusuke Nakauchi, Maimi Higuchi, Jinyu Zhang, Katja Pekrun, Ian Hsu, Amy C. Fan, Toshinobu Nishimura, Carsten T. Charlesworth, Joydeep Bhadury, Toshiya Nishimura, Adam C. Wilkinson, Mark A. Kay, Ravindra Majeti, Hiromitsu Nakauchi
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Histopathological biomarkers for predicting the tumour accumulation of nanomedicines Nat. Biomed. Eng. (IF 28.1) Pub Date : 2024-04-08 Jan-Niklas May, Jennifer I. Moss, Florian Mueller, Susanne K. Golombek, Ilaria Biancacci, Larissa Rizzo, Asmaa Said Elshafei, Felix Gremse, Robert Pola, Michal Pechar, Tomáš Etrych, Svea Becker, Christian Trautwein, Roman D. Bülow, Peter Boor, Ruth Knuechel, Saskia von Stillfried, Gert Storm, Sanyogitta Puri, Simon T. Barry, Volkmar Schulz, Fabian Kiessling, Marianne B. Ashford, Twan Lammers
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Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-08 Siqi Wang, Yujie Liu, Hongfu Guo, Yan Meng, Weini Xiong, Ruihua Liu, Chao Yang
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Machine learning approaches to predict TAS2R receptors for bitterants Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-08 Francesco Ferri, Marco Cannariato, Marco Agostino Deriu, Lorenzo Pallante
Bitter taste involves the detection of diverse chemical compounds by a family of G protein‐coupled receptors, known as taste receptor type 2 (TAS2R). It is often linked to toxins and harmful compounds and in particular bitter taste receptors participate in the regulation of glucose homeostasis, modulation of immune and inflammatory responses, and may have implications for various diseases. Human TAS2Rs
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Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-07 Lijuan Wang, Yingying Guo, Yizhou Shen, Kun Yang, Xue Cai, Bo Zhang, Zhiqiang Liu, Yuguo Zheng
L-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with being extensively studied as the preferred strain. This preference is due to its ability
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Recent advances in design and application of synthetic membraneless organelles Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-06 Li Wan, Yingying Zhu, Wenli Zhang, Wanmeng Mu
Membraneless organelles (MLOs) formed by liquid-liquid phase separation (LLPS) have been extensively studied due to their spatiotemporal control of biochemical and cellular processes in living cells. These findings have provided valuable insights into the physicochemical principles underlying the formation and functionalization of biomolecular condensates, which paves the way for the development of
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Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-06 Zi-Xu Zhang, Ying-Shuang Xu, Zi-Jia Li, Lu-Wei Xu, Wang Ma, Ying-Feng Li, Dong-Sheng Guo, Xiao-Man Sun, He Huang
are marine microorganisms known for their fast growth and ability to store lipids, making them useful for producing polyunsaturated fatty acids (PUFAs), biodiesel, squalene, and carotenoids. However, the high cost of production, mainly due to expensive fermentation components, limits their wider use. A significant challenge in this context is the need to balance production costs with the value of the
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Gene trajectory inference for single-cell data by optimal transport metrics Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-05 Rihao Qu, Xiuyuan Cheng, Esen Sefik, Jay S. Stanley III, Boris Landa, Francesco Strino, Sarah Platt, James Garritano, Ian D. Odell, Ronald Coifman, Richard A. Flavell, Peggy Myung, Yuval Kluger
Single-cell RNA sequencing has been widely used to investigate cell state transitions and gene dynamics of biological processes. Current strategies to infer the sequential dynamics of genes in a process typically rely on constructing cell pseudotime through cell trajectory inference. However, the presence of concurrent gene processes in the same group of cells and technical noise can obscure the true
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Genetically encoding colors and images into bioengineered microbial materials Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-02
Using synthetic biology, we engineered a cellulose-producing bacterium that can produce eumelanin and respond to light, so that it is possible to grow a microbial leather material that is colored black or contains projected black patterns.
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Capturing and modeling cellular niches from dissociated single-cell and spatial data Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-02
Cells interact with their local environment to enact global tissue function. By harnessing gene–gene covariation in cellular neighborhoods from spatial transcriptomics data, the covariance environment (COVET) niche representation and the environmental variational inference (ENVI) data integration method model phenotype–microenvironment interplay and reconstruct the spatial context of dissociated single-cell
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Self-pigmenting textiles grown from cellulose-producing bacteria with engineered tyrosinase expression Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-02 Kenneth T. Walker, Ivy S. Li, Jennifer Keane, Vivianne J. Goosens, Wenzhe Song, Koon-Yang Lee, Tom Ellis
Environmental concerns are driving interest in postpetroleum synthetic textiles produced from microbial and fungal sources. Bacterial cellulose (BC) is a promising sustainable leather alternative, on account of its material properties, low infrastructure needs and biodegradability. However, for alternative textiles like BC to be fully sustainable, alternative ways to dye textiles need to be developed
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The potential of DAOs for funding and collaborative development in the life sciences Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-02 Simone Fantaccini, Laura Grassi, Andrea Rampoldi
VitaDAO funds longevity research through a blockchain-based decentralized autonomous organization (DAO), showcasing the potential of collaborative, transparent and alternative systems while also highlighting the challenges of coordination, regulation, biases and skepticism in reshaping traditional research financing methods.
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Antivenom slithers back to life Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-03
Snakebite treatment still relies on antivenom, a 130-year old technology, but biotech is on the job.
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The covariance environment defines cellular niches for spatial inference Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-02 Doron Haviv, Ján Remšík, Mohamed Gatie, Catherine Snopkowski, Meril Takizawa, Nathan Pereira, John Bashkin, Stevan Jovanovich, Tal Nawy, Ronan Chaligne, Adrienne Boire, Anna-Katerina Hadjantonakis, Dana Pe’er
A key challenge of analyzing data from high-resolution spatial profiling technologies is to suitably represent the features of cellular neighborhoods or niches. Here we introduce the covariance environment (COVET), a representation that leverages the gene–gene covariate structure across cells in the niche to capture the multivariate nature of cellular interactions within it. We define a principled
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Systematic review on carrageenolytic enzymes: From metabolic pathways to applications in biotechnology Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-04 Chengcheng Jiang, Yuqi Ma, Wei Wang, Jingjing Sun, Jianhua Hao, Xiangzhao Mao
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Rethinking 13C-metabolic flux analysis – The Bayesian way of flux inference Metab. Eng. (IF 8.4) Pub Date : 2024-04-04 Axel Theorell, Johann F. Jadebeck, Wolfgang Wiechert, Johnjoe McFadden, Katharina Nöh
Metabolic reaction rates (fluxes) play a crucial role in comprehending cellular phenotypes and are essential in areas such as metabolic engineering, biotechnology, and biomedical research. The state-of-the-art technique for estimating fluxes is metabolic flux analysis using isotopic labelling (C-MFA), which uses a dataset-model combination to determine the fluxes. Bayesian statistical methods are gaining
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Combinatorial metabolic engineering of Bacillus subtilis for de novo production of polymyxin B Metab. Eng. (IF 8.4) Pub Date : 2024-04-04 Hui-Zhong Sun, Qing Li, Wei Shang, Bin Qiao, Qiu-Man Xu, Jing-Sheng Cheng
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Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications Biotechnol. Adv. (IF 16.0) Pub Date : 2024-04-03 Shi-Peng Zhou, Ya-Ping Xue, Yu-Guo Zheng
Nitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids. These enzymatic entities have garnered significant attention across a spectrum of industries, encompassing pharmaceuticals, agrochemicals, and fine chemicals. Moreover, their significance has been accentuated by mounting