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Detection of blaKPC gene among carbapenemase producing Klebsiella pneumoniae isolated from different clinical specimens at tertiary care hospital of Nepal BMC Microbiol. (IF 4.2) Pub Date : 2024-04-25 Rakshya Baral, Reshma Tuladhar, Sarita Manandhar, Anjana Singh, Samendra Sherchan
Klebsiella pneumoniae infections have become a major cause of hospital acquired infection worldwide with the increased rate of acquisition of resistance to antibiotics. Carbapenem resistance mainly among Gram negative is an ongoing problem which causes serious outbreaks dramatically limiting treatment options. This prospective cross-sectional study was designed to detect blaKPC gene from carbapenem
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Survival of highly related ESBL- and pAmpC- producing Escherichia coli in broiler farms identified before and after cleaning and disinfection using cgMLST BMC Microbiol. (IF 4.2) Pub Date : 2024-04-25 Caroline Robé, Michaela Projahn, Katrin Boll, Anja Blasse, Roswitha Merle, Uwe Roesler, Anika Friese
Broiler chickens are frequently colonized with Extended-Spectrum Beta-Lactamase- (ESBL-) and plasmid mediated AmpC Beta-Lactamase- (pAmpC-) producing Enterobacterales, and we are confronted with the potential spread of these resistant bacteria in the food chain, in the environment, and to humans. Research focused on identifying of transmission routes and investigating potential intervention measures
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Genomic characterization and probiotic potential assessment of an exopolysaccharide-producing strain Pediococcus pentosaceus LL-07 isolated from fermented meat BMC Microbiol. (IF 4.2) Pub Date : 2024-04-25 Kuan Lu, Xueya Wang, Ying Zhou, Qiujin Zhu
The genomic information available for Pediococcus pentosaceus is primarily derived from fermented fruits and vegetables, with less information available from fermented meat. P. pentosaceus LL-07, a strain isolated from fermented meat, has the capability of producing exopolysaccharides (EPS). To assess the probiotic attributes of P. pentosaceus LL-07, we conducted whole-genome sequencing (WGS) using
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Gut bacterial and fungal dysbiosis in tuberculosis patients BMC Microbiol. (IF 4.2) Pub Date : 2024-04-25 MeiQing Han, Xia Wang, JiaMin Zhang, Lin Su, Hafiz Muhammad Ishaq, Duan Li, JunWei Cui, HuaJie Zhao, Fan Yang
Recent studies have more focused on gut microbial alteration in tuberculosis (TB) patients. However, no detailed study on gut fungi modification has been reported till now. So, current research explores the characteristics of gut microbiota (bacteria)- and mycobiota (fungi)-dysbiosis in TB patients and also assesses the correlation between the gut microbiome and serum cytokines. It may help to screen
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Production of kojic acid by Aspergillus flavus OL314748 using box-Behnken statistical design and its antibacterial and anticancer applications using molecular docking technique BMC Microbiol. (IF 4.2) Pub Date : 2024-04-25 Ghada Abd-Elmonsef Mahmoud, Abo bakr Abdel Shakor, Nahla A. Kamal-Eldin, Abdel-Naser A. Zohri
Kojic acid is a wonderful fungal secondary metabolite that has several applications in the food, medical, and agriculture sectors. Many human diseases become resistant to normal antibiotics and normal treatments. We need to search for alternative treatment sources and understand their mode of action. Aspergillus flavus ASU45 (OL314748) was isolated from the caraway rhizosphere as a non-aflatoxin producer
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Microbiome signatures associated with clinical stages of gastric Cancer: whole metagenome shotgun sequencing study BMC Microbiol. (IF 4.2) Pub Date : 2024-04-24 Sohyun Jeong, Yi-Tyng Liao, Min-Hsuan Tsai, Yao-Kuang Wang, I-Chen Wu, Chung-Jung Liu, Ming-Shun Wu, Tze-Sian Chan, Ming-Yao Chen, Ping-Jen Hu, Wei-Yu Kao, Hsiang-Chin Liu, Ming-Ju Tsai, Cheng-Yuan Liu, Chun-Chao Chang, Deng-Chyang Wu, Yi-Hsiang Hsu
Gastric cancer is one of the global health concerns. A series of studies on the stomach have confirmed the role of the microbiome in shaping gastrointestinal diseases. Delineation of microbiome signatures to distinguish chronic gastritis from gastric cancer will provide a non-invasive preventative and treatment strategy. In this study, we performed whole metagenome shotgun sequencing of fecal samples
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The characteristics of microbiome in the upper respiratory tract of COVID-19 patients BMC Microbiol. (IF 4.2) Pub Date : 2024-04-24 Xilong Zhang, Nadira Nurxat, Jueraiti Aili, Yakupu Yasen, Qichen Wang, Qian Liu
Co-infection with other pathogens in coronavirus disease 2019 (COVID-19) patients exacerbates disease severity and impacts patient prognosis. Clarifying the exact pathogens co-infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is premise of the precise treatment for COVID-19 patients. Sputum samples were collected from 17 patients in the COVID-19 positive group and 18 patients
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Occurrence, antibiotic resistance profiles and associated risk factors of Klebsiella pneumoniae in poultry farms in selected districts of Somalia Reginal State, Ethiopia BMC Microbiol. (IF 4.2) Pub Date : 2024-04-24 Mohamed Abdi Kahin, Abdimalik Hussein mohamed, Ahmed Abdi Mohomed, Mubarik Ali Hassan, Haben Fesseha Gebremeskel, Isayas Asefa Kebede
Klebsiella pneumoniae is an opportunistic infection that causes production losses and death in the chicken industry. A cross-sectional study was conducted on exotic chicken breeds reared at the Jigjiga poultry farm from November 2022 to May 2023 to estimate the occurrence, associated risk factors, and antimicrobial susceptibility profiles of Klebsiella pneumoniae. The chickens were selected using systematic
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High prevalence of multidrug-resistant Enterobacterales carrying extended-spectrum beta-lactamase and AmpC genes isolated from neonatal sepsis in Ahvaz, Iran BMC Microbiol. (IF 4.2) Pub Date : 2024-04-24 Sima Mansouri, Mohammad Savari, Arash Malakian, Effat Abbasi Montazeri
In the recent years, multidrug resistant (MDR) neonatal septicemia-causing Enterobacterales has been dramatically increased due to the extended-spectrum beta-lactamases (ESBLs) and AmpC enzymes. This study aimed to assess the antibiotic resistance pattern, prevalence of ESBLs/AmpC beta-lactamase genes, and Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) fingerprints
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Phenotypic and molecular characterization of β-lactamase-producing Klebsiella species among children discharged from hospital in Western Kenya BMC Microbiol. (IF 4.2) Pub Date : 2024-04-23 Doreen Rwigi, Andrew K. Nyerere, Mame M. Diakhate, Kevin Kariuki, Kirkby D. Tickell, Timothy Mutuma, Stephanie N. Tornberg, Olusegun O. Soge, Judd L. Walson, Benson Singa, Samuel Kariuki, Patricia B. Pavlinac, Polycarp Mogeni
The emergence and spread of β-lactamase-producing Klebsiella spp. has been associated with a substantial healthcare burden resulting in therapeutic failures. We sought to describe the proportion of phenotypic resistance to commonly used antibiotics, characterize β-lactamase genes among isolates with antimicrobial resistance (AMR), and assess the correlates of phenotypic AMR in Klebsiella spp. isolated
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Exertional heat stroke-induced changes in gut microbiota cause cognitive impairment in mice BMC Microbiol. (IF 4.2) Pub Date : 2024-04-23 Jiangang Xie, Linxiao Wang, Yunyun Xu, Yuexiang Ma, Lingqin Zhang, Wen Yin, Yang Huang
The incidence of exertional heat stroke (EHS) escalates during periods of elevated temperatures, potentially leading to persistent cognitive impairment postrecovery. Currently, effective prophylactic or therapeutic measures against EHS are nonexistent. The selection of days 14 and 23 postinduction for detailed examination was guided by TEM of neuronal cells and HE staining of intestinal villi and the
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Altered microbiome of serum exosomes in patients with acute and chronic cholecystitis BMC Microbiol. (IF 4.2) Pub Date : 2024-04-20 Qing Zhu, Min-Xian Li, Ming-Chin Yu, Qi-Wen Ma, Ming-Jie Huang, Chun-Wei Lu, Chun-Bing Chen, Wen-Hung Chung, Chih-Jung Chang
This study aimed to investigate the differences in the microbiota composition of serum exosomes from patients with acute and chronic cholecystitis. Exosomes were isolated from the serum of cholecystitis patients through centrifugation and identified and characterized using transmission electron microscopy and nano-flow cytometry. Microbiota analysis was performed using 16S rRNA sequencing. Compared
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Saliva‑microbiome‑derived signatures: expected to become a potential biomarker for pulmonary nodules (MCEPN-1) BMC Microbiol. (IF 4.2) Pub Date : 2024-04-20 Yifeng Ren, Qiong Ma, Xiao Zeng, Chunxia Huang, Shiyan Tan, Xi Fu, Chuan Zheng, Fengming You, Xueke Li
Oral microbiota imbalance is associated with the progression of various lung diseases, including lung cancer. Pulmonary nodules (PNs) are often considered a critical stage for the early detection of lung cancer; however, the relationship between oral microbiota and PNs remains unknown. We conducted a ‘Microbiome with pulmonary nodule series study 1’ (MCEPN-1) where we compared PN patients and healthy
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The gut microbiota facilitate their host tolerance to extreme temperatures BMC Microbiol. (IF 4.2) Pub Date : 2024-04-20 Ziguang Wang, Yujie Wu, Xinxin Li, Xiaowen Ji, Wei Liu
Exposure to extreme cold or heat temperature is one leading cause of weather-associated mortality and morbidity in animals. Emerging studies demonstrate that the microbiota residing in guts act as an integral factor required to modulate host tolerance to cold or heat exposure, but common and unique patterns of animal-temperature associations between cold and heat have not been simultaneously examined
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Establishment of an in vitro model of monocyte-like THP-1 cells for trained immunity induced by bacillus Calmette-Guérin BMC Microbiol. (IF 4.2) Pub Date : 2024-04-20 Jin-Chuan Xu, Kang Wu, Rui-qing Ma, Jian-hui Li, Jie Tao, Zhidong Hu, Xiao-Yong Fan
Mycobacteria bloodstream infections are common in immunocompromised people and usually have disastrous consequences. As the primary phagocytes in the bloodstream, monocytes and neutrophils play critical roles in the fight against bloodstream mycobacteria infections. In contrast to macrophages, the responses of monocytes infected with the mycobacteria have been less investigated. In this study, we first
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The distinct cell physiology of Bradyrhizobium at the population and cellular level BMC Microbiol. (IF 4.2) Pub Date : 2024-04-20 Ian F. Medici, Leila Bartrolí, Francisco F. Guaimas, Fabiana R. Fulgenzi, Charo Luciana Molina, Ignacio Enrique Sánchez, Diego J. Comerci, Elías Mongiardini, Alfonso Soler-Bistué
The α-Proteobacteria belonging to Bradyrhizobium genus are microorganisms of extreme slow growth. Despite their extended use as inoculants in soybean production, their physiology remains poorly characterized. In this work, we produced quantitative data on four different isolates: B. diazoefficens USDA110, B. diazoefficiens USDA122, B. japonicum E109 and B. japonicum USDA6 which are representative of
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Decoding the role of oxidative stress resistance and alternative carbon substrate assimilation in the mature biofilm growth mode of Candida glabrata BMC Microbiol. (IF 4.2) Pub Date : 2024-04-20 Khem Raj, Dhiraj Paul, Praveen Rishi, Geeta Shukla, Dhiraj Dhotre, YogeshSouche
Biofilm formation is viewed as a vital mechanism in C. glabrata pathogenesis. Although, it plays a significant role in virulence but transcriptomic architecture and metabolic pathways governing the biofilm growth mode of C. glabrata remain elusive. The present study intended to investigate the genes implicated in biofilm growth phase of C. glabrata through global transcriptomic approach. Functional
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Occurrence, antimicrobial susceptibility, and resistance genes of Staphylococcus aureus in milk and milk products in the Arsi highlands of Ethiopia BMC Microbiol. (IF 4.2) Pub Date : 2024-04-16 Abiot Deddefo, Gezahegne Mamo, Minda Asfaw, Adem Edao, Adem Hiko, Dereje Fufa, Mohammed Jafer, Melaku Sombo, Kebede Amenu
In Ethiopia, milk production and handling practices often lack proper hygiene measures, leading to the potential contamination of milk and milk products with Staphylococcus aureus (S. aureus), including methicillin-resistant strains, posing significant public health concerns. This study aimed to investigate the occurrence, antimicrobial susceptibility profiles and presence of resistance genes in S
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Based on molecular docking and real-time PCR technology, the two-component system Bae SR was investigated on the mechanism of drug resistance in CRAB BMC Microbiol. (IF 4.2) Pub Date : 2024-04-15 Beizhen Pan, Yuefeng Wang, Jiansheng Su, Yan Liu, Jifei Yang, Yujiao Zhou, Liyuan Sun
This study aimed to explore the role of the two-component system Bae SR in the mechanism of drug resistance in carbapenem-resistant A. baumannii (CRAB) using molecular docking and real-time polymerase chain reaction (PCR). The two-component system Bae SR of Acinetobacter baumannii was subjected to molecular docking with imipenem, meropenem, and levofloxacin. Antibacterial assays and fluorescence quantitative
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Genomic characterization and related functional genes of γ- poly glutamic acid producing Bacillus subtilis BMC Microbiol. (IF 4.2) Pub Date : 2024-04-15 Jiayue Zhu, Xue Wang, Jianan Zhao, Fang Ji, Jun Zeng, Yanwen Wei, LiLi Xu, Guoying Dong, Xingyuan Ma, Chengmin Wang
γ- poly glutamic acid (γ-PGA), a high molecular weight polymer, is synthesized by microorganisms and secreted into the extracellular space. Due to its excellent performance, γ-PGA has been widely used in various fields, including food, biomedical and environmental fields. In this study, we screened natto samples for two strains of Bacillus subtilis N3378-2at and N3378-3At that produce γ-PGA. We then
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In silico analysis of intestinal microbial instability and symptomatic markers in mice during the acute phase of severe burns BMC Microbiol. (IF 4.2) Pub Date : 2024-04-15 Bochen Hou, Honglan Zhang, Lina Zhou, Biao Hu, Wenyi Tang, Bo Ye, Cui Wang, Yongmei Xu, Lingyun Zou, Jun Hu
Severe burns may alter the stability of the intestinal flora and affect the patient’s recovery process. Understanding the characteristics of the gut microbiota in the acute phase of burns and their association with phenotype can help to accurately assess the progression of the disease and identify potential microbiota markers. We established mouse models of partial thickness deep III degree burns and
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The effect of white grub (Maladera Verticalis) larvae feeding on rhizosphere microbial characterization of aerobic rice (Oryza sativa L.) in Puer City, Yunnan Province, China BMC Microbiol. (IF 4.2) Pub Date : 2024-04-15 Guang Wang, Zhengfei Li, Baoyun Yang, Huquan Yang, Yujie Zhang, Qingping Zeng, Chaojianping Yan, Yanyan He, Yuejin Peng, Wenqian Wang, Bin Chen, Guangzu Du
Rhizosphere microorganisms are vital in plants’ growth and development and these beneficial microbes are recruited to the root-zone soil when experiencing various environmental stresses. However, the effect of white grub (Maladera verticalis) larvae feeding on the structure and function of rhizosphere microbial communities of aerobic rice (Oryza sativa L.) is unclear. In this study, we compared physicochemical
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Characterization of genes related to the efflux pump and porin in multidrug-resistant Escherichia coli strains isolated from patients with COVID-19 after secondary infection BMC Microbiol. (IF 4.2) Pub Date : 2024-04-10 Aryan R. Ganjo, Salah Tofik Jalal Balaky, Ahang Hasan Mawlood, Sakar B. Smail, Nazar P. Shabila
Escherichia coli (E. coli) is a multidrug resistant opportunistic pathogen that can cause secondary bacterial infections in patients with COVID-19. This study aimed to determine the antimicrobial resistance profile of E. coli as a secondary bacterial infection in patients with COVID-19 and to assess the prevalence and characterization of genes related to efflux pumps and porin. A total of 50 nonduplicate
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Correction: Uncovering the complexity of childhood undernutrition through strain‑level analysis of the gut microbiome BMC Microbiol. (IF 4.2) Pub Date : 2024-04-08 Bingmei Chang, Wenjie Zhang, Yinan Wang, Yuanzheng Zhang, Shilin Zhong, Peng Gao, Lili Wang, Zicheng Zhao
Correction: BMC Microbiol 24, 73 (2024) https://doi.org/10.1186/s12866-024-03211-w Following publication of the original article [1], the authors identified an error in the order of author names in author list and its affiliations. The incorrect and correct author list, as well as its affiliation, are provided below. The original article has been corrected. The incorrect author list: Lili Wang1, Wenjie
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Optimization of fermentation conditions and medium components for chrysomycin a production by Streptomyces sp. 891-B6 BMC Microbiol. (IF 4.2) Pub Date : 2024-04-06 Zhe Hu, Qiangang Weng, Zhehui Cai, Huawei Zhang
Chrysomycin A (CA) is a promising antibiotic for treatment of Gram-positive bacterial infections and cancers. In order to enhance CA yield, optimization of fermentation conditions and medium components was carried out on strain Streptomyces sp. 891-B6, an UV-induced mutant with improved CA titer compared with its wide-type marine strain 891. Using one-way experiment, the optimal fermentation conditions
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Integrative metagenomic analysis reveals distinct gut microbial signatures related to obesity BMC Microbiol. (IF 4.2) Pub Date : 2024-04-05 Xinliang Hu, Chong Yu, Yuting He, Songling Zhu, Shuang Wang, Ziqiong Xu, Shaohui You, Yanlei Jiao, Shu-Lin Liu, Hongxia Bao
Obesity is a metabolic disorder closely associated with profound alterations in gut microbial composition. However, the dynamics of species composition and functional changes in the gut microbiome in obesity remain to be comprehensively investigated. In this study, we conducted a meta-analysis of metagenomic sequencing data from both obese and non-obese individuals across multiple cohorts, totaling
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Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen BMC Microbiol. (IF 4.2) Pub Date : 2024-04-04 Katja Mertens-Scholz, Amira A. Moawad, Elisabeth M. Liebler-Tenorio, Andrea Helming, Jennifer Andrack, Peter Miethe, Heinrich Neubauer, Mathias W. Pletz, Ina-Gabriele Richter
Q fever, a worldwide-occurring zoonotic disease, can cause economic losses for public and veterinary health systems. Vaccines are not yet available worldwide and currently under development. In this regard, it is important to produce a whole cell antigen, with preserved structural and antigenic properties and free of chemical modifications. Thus, inactivation of Coxiella burnetii with ultraviolet light
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Neutrophil extracellular traps formation: effect of Leishmania major promastigotes and salivary gland homogenates of Phlebotomus papatasi in human neutrophil culture BMC Microbiol. (IF 4.2) Pub Date : 2024-04-04 Fahimeh Firouzjaie, Niloofar Taghipour, Amir Ahmad Akhavan, Seyyed Javad Seyyed Tabaei, Soheila Rouhani, Maryam Shirazian, Ameneh Koochaki, Mahboubeh Fatemi, Nariman Mosaffa, Vahideh Moin Vaziri
Leishmaniasis as a neglected tropical disease (NTD) is caused by the inoculation of Leishmania parasites via the bite of phlebotomine sand flies. After an infected bite, a series of innate and adaptive immune responses occurs, among which neutrophils can be mentioned as the initiators. Among the multiple functions of these fighting cells, neutrophil extracellular traps (NETs) were studied in the presence
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Assessment of bacterial profile, antimicrobial susceptibility status, and associated factors of isolates among hospitalized patients at Dessie Comprehensive Specialized Hospital, Northeast Ethiopia BMC Microbiol. (IF 4.2) Pub Date : 2024-04-04 Assefa Sisay, Abdurahaman Seid, Selamyhun Tadesse, Wagaw Abebe, Agumas Shibabaw
Antimicrobial resistant bacteria among hospitalized patients are becoming a major public health threat worldwide, mainly in developing countries. Infections by these multidrug resistant pathogens cause high rate of mortality, prolong hospital stays, and affect individual and country economies in greater amounts. Thus, this study aimed to assess the bacterial profile, antimicrobial susceptibility status
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A review of emerging health threats from zoonotic New World mammarenaviruses BMC Microbiol. (IF 4.2) Pub Date : 2024-04-04 Arianna Lendino, Adrian A. Castellanos, David M. Pigott, Barbara A. Han
Despite repeated spillover transmission and their potential to cause significant morbidity and mortality in human hosts, the New World mammarenaviruses remain largely understudied. These viruses are endemic to South America, with animal reservoir hosts covering large geographic areas and whose transmission ecology and spillover potential are driven in part by land use change and agriculture that put
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Impact of Limosilactobacillus fermentum probiotic treatment on gut microbiota composition in sahiwal calves with rotavirus diarrhea: A 16S metagenomic analysis study” BMC Microbiol. (IF 4.2) Pub Date : 2024-04-04 Nadeem Murtaza, Muhammad Nawaz, Tahir Yaqub, Asim Khalid Mehmood
Diarrhea poses a major threat to bovine calves leading to mortality and economic losses. Among the causes of calf diarrhea, bovine rotavirus is a major etiological agent and may result in dysbiosis of gut microbiota. The current study was designed to investigate the effect of probiotic Limosilactobacillus fermentum (Accession No.OR504458) on the microbial composition of rotavirus-infected calves using
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Genetic characterizations of Cryptosporidium spp. from children with or without diarrhea in Wenzhou, China: high probability of zoonotic transmission BMC Microbiol. (IF 4.2) Pub Date : 2024-04-04 Wei Zhao, Guangxu Ren, Weiyan Jiang, Long Wang, Jiayang Wang, Zhongying Yuan, Lanzhu Yan, Yongtai Li, Yanbin Sun, Xinjie Xue, Yanyan Jiang, Gang Lu, Huicong Huang
Cryptosporidium is a highly pathogenic parasite responsible for diarrhea in children worldwide. Here, the epidemiological status and genetic characteristics of Cryptosporidium in children with or without diarrhea were investigated with tracking of potential sources in Wenzhou City, China. A total of 1032 children were recruited, 684 of whom had diarrhea and 348 without, from Yuying Children’s Hospital
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Effect of stress urinary incontinence on vaginal microbial communities BMC Microbiol. (IF 4.2) Pub Date : 2024-04-04 Man Zhang, Yanhua Zhou, Siqi Yao, Yiming Zhao, Syeda Sundas Batool, Jing Huang, Li Jiang, Dayu Yan, Wenguang Yan, Zheng Yu
Postpartum women often experience stress urinary incontinence (SUI) and vaginal microbial dysbiosis, which seriously affect women’s physical and mental health. Understanding the relationship between SUI and vaginal microbiota composition may help to prevent vaginal diseases, but research on the potential association between these conditions is limited. This study employed 16S rRNA gene sequencing to
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Hospital distribution, seasonality, time trends and antifungal susceptibility profiles of all Aspergillus species isolated from clinical samples from 2015 to 2022 in a tertiary care hospital BMC Microbiol. (IF 4.2) Pub Date : 2024-04-03 Iacopo Franconi, Cosmeri Rizzato, Emilia Ghelardi, Antonella Lupetti
Aspergillus species cause a variety of serious clinical conditions with increasing trend in antifungal resistance. The present study aimed at evaluating hospital epidemiology and antifungal susceptibility of all isolates recorded in our clinical database since its implementation. Data on date of isolation, biological samples, patients’ age and sex, clinical settings, and antifungal susceptibility tests
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Comparative analysis of proteomic adaptations in Enterococcus faecalis and Enterococcus faecium after long term bile acid exposure BMC Microbiol. (IF 4.2) Pub Date : 2024-04-03 Annika Dreyer, Christof Lenz, Uwe Groß, Wolfgang Bohne, Andreas Erich Zautner
All gastrointestinal pathogens, including Enterococcus faecalis and Enterococcus faecium, undergo adaptation processes during colonization and infection. In this study, we investigated by data-independent acquisition mass spectrometry (DIA-MS) two crucial adaptations of these two Enterococcus species at the proteome level. Firstly, we examined the adjustments to cope with bile acid concentrations at
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Influence of PhoPQ and PmrAB two component system alternations on colistin resistance from non-mcr colistin resistant clinical E. Coli strains BMC Microbiol. (IF 4.2) Pub Date : 2024-04-02 Ching-Hsun Wang, L. Kristopher Siu, Feng-Yee Chang, Yu-Kuo Tsai, Li-Yueh Huang, Jung-Chung Lin
The current understanding of acquired chromosomal colistin resistance mechanisms in Enterobacterales primarily involves the disruption of the upstream PmrAB and PhoPQ two-component system (TCS) control caused by mutations in the regulatory genes. Interestingly, previous studies have yielded conflicting results regarding the interaction of regulatory genes related to colistin resistance in Escherichia
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Staphylococcus aureus foldase PrsA contributes to the folding and secretion of protein A BMC Microbiol. (IF 4.2) Pub Date : 2024-04-02 Mei-Hui Lin, Chao-Chin Liu, Chiao-Wen Lu, Jwu-Ching Shu
Staphylococcus aureus secretes a variety of proteins including virulence factors that cause diseases. PrsA, encoded by many Gram-positive bacteria, is a membrane-anchored lipoprotein that functions as a foldase to assist in post-translocational folding and helps maintain the stability of secreted proteins. Our earlier proteomic studies found that PrsA is required for the secretion of protein A, an
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Transcriptional dynamics during Rhodococcus erythropolis infection with phage WC1 BMC Microbiol. (IF 4.2) Pub Date : 2024-04-01 Dana L. Willner, Sudip Paudel, Andrew D. Halleran, Grace E. Solini, Veronica Gray, Margaret S. Saha
Belonging to the Actinobacteria phylum, members of the Rhodococcus genus thrive in soil, water, and even intracellularly. While most species are non-pathogenic, several cause respiratory disease in animals and, more rarely, in humans. Over 100 phages that infect Rhodococcus species have been isolated but despite their importance for Rhodococcus ecology and biotechnology applications, little is known
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A hypervirulent Acinetobacter baumannii strain has robust anti-phagocytosis ability BMC Microbiol. (IF 4.2) Pub Date : 2024-04-01 Yan Li, Mohan Jv, Yuan Zhuang, Xu Zhao, Xiaoxiong Hu
Acinetobacter baumannii (A. baumannii) is associated with both hospital-acquired infections (HAP) and community-acquired pneumonia (CAP). In this study, we present a novel CAP-associated A. baumannii (CAP-AB) strain causing severe pneumonia in an afore healthy male patient without underlying conditions. Subsequently, we investigated the pathogenicity and immunogenicity of this CAP-AB strain using a
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Restoration of gut dysbiosis through Clostridium butyricum and magnesium possibly balance blood glucose levels: an experimental study BMC Microbiol. (IF 4.2) Pub Date : 2024-04-01 Hafiz Muhammad Ubaid Tayyib, Amjed Ali, Shaista Jabeen, Habib-ur-Rehman, Hafsa Kamran, Majed A. Bajaber, Muhammad Usman, Xiao Zhang
Diabetes mellitus (DM) is a chronic metabolic disorder characterized by an elevated level of blood glucose due to the absence of insulin secretion, ineffectiveness, or lack of uptake of secreted insulin in the body. The improperly diagnosed and poorly managed DM can cause severe damage to organs in the body like the nerves, eyes, heart, and kidneys. This study was aimed at investigating the effect
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Bacillus subtilis SOM8 isolated from sesame oil meal for potential probiotic application in inhibiting human enteropathogens BMC Microbiol. (IF 4.2) Pub Date : 2024-03-28 Zhongtian Zhao, Wenrui Li, The Thien Tran, Say Chye Joachim Loo
While particular strains within the Bacillus species, such as Bacillus subtilis, have been commercially utilised as probiotics, it is critical to implement screening assays and evaluate the safety to identify potential Bacillus probiotic strains before clinical trials. This is because some Bacillus species, including B. cereus and B. anthracis, can produce toxins that are harmful to humans. In this
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Promiscuous, persistent and problematic: insights into current enterococcal genomics to guide therapeutic strategy BMC Microbiol. (IF 4.2) Pub Date : 2024-03-28 David Hourigan, Ewelina Stefanovic, Colin Hill, R. Paul Ross
Vancomycin-resistant enterococci (VRE) are major opportunistic pathogens and the causative agents of serious diseases, such as urinary tract infections and endocarditis. VRE strains mainly include species of Enterococcus faecium and E. faecalis which can colonise the gastrointestinal tract (GIT) of patients and, following growth and persistence in the gut, can transfer to blood resulting in systemic
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Comparison of integron mediated antimicrobial resistance in clinical isolates of Escherichia coli from urinary and bacteremic sources BMC Microbiol. (IF 4.2) Pub Date : 2024-03-28 Gauri Kumar, Keshava Balakrishna, Chiranjay Mukhopadhyay, Vandana Kalwaje Eshwara
Antimicrobial resistance (AMR) is a global threat driven mainly by horizontal gene transfer (HGT) mechanisms through mobile genetic elements (MGEs) including integrons. The variable region (VR) of an integron can acquire or excise gene cassettes (GCs) that confer resistance to antibiotics based on the selection pressure. Escherichia coli plays a significant role in the genetic transfer of resistance
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Structure predictions and functional insights into Amidase_3 domain containing N-acetylmuramyl-L-alanine amidases from Deinococcus indicus DR1 BMC Microbiol. (IF 4.2) Pub Date : 2024-03-26 Malvika Modi, Menaka Thambiraja, Archana Cherukat, Ragothaman M Yennamalli, Richa Priyadarshini
N-acetylmuramyl-L-alanine amidases are cell wall modifying enzymes that cleave the amide bond between the sugar residues and stem peptide in peptidoglycan. Amidases play a vital role in septal cell wall cleavage and help separate daughter cells during cell division. Most amidases are zinc metalloenzymes, and E. coli cells lacking amidases grow as chains with daughter cells attached to each other. In
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Profile of non-tuberculous mycobacteria amongst tuberculosis presumptive people in Cameroon BMC Microbiol. (IF 4.2) Pub Date : 2024-03-26 Valerie Flore Donkeng Donfack, Thierry Anicet Zemo Fokou, Lazare Eric Noche Wadje, Yves Le Grand Napa Tchuedji, Yvonne Josiane Djieugoue, Sorelle Nguimfack Teagho, Brenda Shile Takong, Yannick Patrick Assolo, Suzanne Magloire Ongboulal, Yannick Willy Kamdem Simo, Stanley Nkemnji Awungafac, Sara Eyangoh
Cameroon is a tuberculosis (TB) burden country with a 12% positivity among TB presumptive cases. Of the presumptive cases with a negative TB test, some are infected with Non-tuberculous Mycobacteria (NTM). However, the diagnosis of NTM infections remains difficult due to the lack of tools in many laboratories, particularly in resource limited laboratories and remote setting. The present study was undertaken
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In vitro investigation of relationship between quorum-sensing system genes, biofilm forming ability, and drug resistance in clinical isolates of Pseudomonas aeruginosa BMC Microbiol. (IF 4.2) Pub Date : 2024-03-25 Jaber Hemmati, Mohsen Nazari, Fatemeh Sadat Abolhasani, Amjad Ahmadi, Babak Asghari
Pseudomonas aeruginosa is an opportunistic pathogen in the health-care systems and one of the primary causative agents with high mortality in hospitalized patients, particularly immunocompromised. The limitation of effective antibiotic administration in multidrug-resistant and extensively drug-resistant P. aeruginosa isolates leads to the development of nosocomial infections and health problems. Quorum
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Relationship between heart failure and intestinal inflammation in infants with congenital heart disease BMC Microbiol. (IF 4.2) Pub Date : 2024-03-25 Qi-Liang Zhang, Xiu-Hua Chen, Si-Jia Zhou, Yu-Qing Lei, Qiang Chen, Hua Cao
The association between heart failure (HF) and intestinal inflammation caused by a disturbed intestinal microbiota in infants with congenital heart disease (CHD) was investigated. Twenty infants with HF and CHD who were admitted to our hospital between October 2021 and March 2022 were included in this study. Twenty age- and sex-matched infants without HF at our hospital were selected as the control
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Clostridium butyricum inhibits the inflammation in children with primary nephrotic syndrome by regulating Th17/Tregs balance via gut-kidney axis BMC Microbiol. (IF 4.2) Pub Date : 2024-03-23 Ting Li, Xiaolong Ma, Ting Wang, Wenyan Tian, Jian Liu, Wenke Shen, Yuanyuan Liu, Yiwei Li, Xiaoxu Zhang, Junbai Ma, Xiaoxia Zhang, Jinhai Ma, Hao Wang
Primary nephrotic syndrome (PNS) is a common glomerular disease in children. Clostridium butyricum (C. butyricum), a probiotic producing butyric acid, exerts effective in regulating inflammation. This study was designed to elucidate the effect of C. butyricum on PNS inflammation through the gut-kidney axis. BALB/c mice were randomly divided into 4 groups: normal control group (CON), C. butyricum control
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Human-derived bacterial strains mitigate colitis via modulating gut microbiota and repairing intestinal barrier function in mice BMC Microbiol. (IF 4.2) Pub Date : 2024-03-23 Juanjuan Dai, Mingjie Jiang, Xiaoxin Wang, Tao Lang, Leilei Wan, Jingjing Wang
Unbalanced gut microbiota is considered as a pivotal etiological factor in colitis. Nevertheless, the precise influence of the endogenous gut microbiota composition on the therapeutic efficacy of probiotics in colitis remains largely unexplored. In this study, we isolated bacteria from fecal samples of a healthy donor and a patient with ulcerative colitis in remission. Subsequently, we identified three
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In vitro and in silico studies of enterobactin-inspired Ciprofloxacin and Fosfomycin first generation conjugates on the antibiotic resistant E. coli OQ866153 BMC Microbiol. (IF 4.2) Pub Date : 2024-03-22 Mohamed T. Khazaal, Ahmed H. I. Faraag, Hoda H. El-Hendawy
The emergence of antimicrobial resistance in bacterial pathogens is a growing concern worldwide due to its impact on the treatment of bacterial infections. The "Trojan Horse" strategy has been proposed as a potential solution to overcome drug resistance caused by permeability issues. The objective of our research was to investigate the bactericidal activity and mechanism of action of the "Trojan Horse"
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HPV-associated cervicovaginal microbiome and host metabolome characteristics BMC Microbiol. (IF 4.2) Pub Date : 2024-03-22 Yao Zhang, Xu Wu, Dan Li, Rong Huang, Xiangyu Deng, Mingxing Li, Fukuan Du, Yueshui Zhao, Jing Shen, Yu Chen, Pingxiu Zhang, Congcui Hu, Zhangang Xiao, Qinglian Wen
Cervicovaginal microbiome plays an important role in the persistence of HPV infection and subsequent disease development. However, cervicovaginal microbiota varied cross populations with different habits and regions. Identification of population-specific biomarkers from cervicovaginal microbiota and host metabolome axis may support early detection or surveillance of HPV-induced cervical disease at
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Transcriptomic and physiological analyses of Trichoderma citrinoviride HT-1 assisted phytoremediation of Cd contaminated water by Phragmites australis BMC Microbiol. (IF 4.2) Pub Date : 2024-03-21 DaWei Chen, YiHan Wang, Ni Li, YaLi Huang, YiFan Mao, XiaoJun Liu, YaRong Du, Kun Sun
Plant growth promoting microbe assisted phytoremediation is considered a more effective approach to rehabilitation than the single use of plants, but underlying mechanism is still unclear. In this study, we combined transcriptomic and physiological methods to explore the mechanism of plant growth promoting microbe Trichoderma citrinoviride HT-1 assisted phytoremediation of Cd contaminated water by
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Long-term push–pull cropping system shifts soil and maize-root microbiome diversity paving way to resilient farming system BMC Microbiol. (IF 4.2) Pub Date : 2024-03-18 Abdul A. Jalloh, Fathiya Mbarak Khamis, Abdullahi Ahmed Yusuf, Sevgan Subramanian, Daniel Munyao Mutyambai
The soil biota consists of a complex assembly of microbial communities and other organisms that vary significantly across farming systems, impacting soil health and plant productivity. Despite its importance, there has been limited exploration of how different cropping systems influence soil and plant root microbiomes. In this study, we investigated soil physicochemical properties, along with soil
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Pretreatment with an antibiotics cocktail enhances the protective effect of probiotics by regulating SCFA metabolism and Th1/Th2/Th17 cell immune responses BMC Microbiol. (IF 4.2) Pub Date : 2024-03-18 Jing Xu, Haoming Xu, Xue Guo, Hailan Zhao, Jiaqi Wang, Jianhong Li, Jie He, Hongli Huang, Chen Huang, Chong Zhao, Yingfei Li, Youlian Zhou, Yao Peng, Yuqiang Nie
Probiotics are a potentially effective therapy for inflammatory bowel disease (IBD); IBD is linked to impaired gut microbiota and intestinal immunity. However, the utilization of an antibiotic cocktail (Abx) prior to the probiotic intervention remains controversial. This study aims to identify the effect of Abx pretreatment from dextran sulfate sodium (DSS)-induced colitis and to evaluate whether Abx
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High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice BMC Microbiol. (IF 4.2) Pub Date : 2024-03-16 Fengjiao Li, Shuo Yang, Ji Ma, Xiaowen Zhao, Meng Chen, Ye Wang
To investigate the differences in bacterial and fungal community structure and diversity in conjunctival tissue of healthy and diabetic mice. RNA-seq assays and high-throughput sequencing of bacterial 16 S rDNA and fungal internal transcribed spacer (ITS) gene sequences were used to identify differentially expressed host genes and fungal composition profiles in conjunctival tissues of diabetic BKS-db/db
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High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China BMC Microbiol. (IF 4.2) Pub Date : 2024-03-16 Qing Zhan, Gaoqin Teng, Weiwei Chen, Xiao Yu
Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging global health threat, resulting in significant morbidity and mortality worldwide. This study aims to determine the molecular characteristics and antimicrobial susceptibility of 263 MRSA isolates in Zhejiang Province, east China. From 2014 to 2019, a total of 263 MRSA isolates from bloodstream infections (BSIs) were collected from 6
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Characteristics of the oral and gastric microbiome in patients with early-stage intramucosal esophageal squamous cell carcinoma BMC Microbiol. (IF 4.2) Pub Date : 2024-03-15 Han Chen, Xingzhou Jiang, Fengyi Zhu, Ruoyun Yang, Xin Yu, Xiaoying Zhou, Nana Tang
Oral microbiome dysbacteriosis has been reported to be associated with the pathogenesis of advanced esophageal cancer. However, few studies investigated the potential role of oral and gastric microbiota in early-stage intramucosal esophageal squamous carcinoma (EIESC). A total of 104 samples were collected from 31 patients with EIESC and 21 healthy controls. The compositions of oral and gastric microbiota
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The potential role of Listeria monocytogenes in promoting colorectal adenocarcinoma tumorigenic process BMC Microbiol. (IF 4.2) Pub Date : 2024-03-15 Giulia Baldelli, Mauro De Santi, Collins Njie Ateba, Giorgia Cifola, Giulia Amagliani, Christ-Donald Kaptchouang Tchatchouang, Peter Kotsoana Montso, Giorgio Brandi, Giuditta Fiorella Schiavano
Listeria monocytogenes is a foodborne pathogen, which can cause a severe illness, especially in people with a weakened immune system or comorbidities. The interactions between host and pathogens and between pathogens and tumor cells have been debated in recent years. However, it is still unclear how bacteria can interact with tumor cells, and if this interaction can affect tumor progression and therapy
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Evaluation of clinical characteristics and risk factors associated with Chlamydia psittaci infection based on metagenomic next-generation sequencing BMC Microbiol. (IF 4.2) Pub Date : 2024-03-13 Lei Yuan, Qiang Chen, Xin Yu Zhu, Lan Min Lai, Rui Zhao, Yang Liu
Psittacosis is a zoonosis caused by Chlamydia psittaci, the clinical manifestations of Psittacosis range from mild illness to fulminant severe pneumonia with multiple organ failure. This study aimed to evaluate the clinical characteristics of Chlamydia psittaci infection diagnosed based on metagenomic next-generation sequencing(mNGS), as well as the risk factors affecting the progress of Chlamydia
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Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods BMC Microbiol. (IF 4.2) Pub Date : 2024-03-11 Tran Thi Dieu Thuy, Hsu-Feng Lu, Carl Jay Ballena Bregente, Fong-Chi Annabelle Huang, Pei-Chun Tu, Cheng-Yen Kao
Antimicrobial peptides, such as bacteriocin, produced by probiotics have become a promising novel class of therapeutic agents for treating infectious diseases. Selected lactic acid bacteria (LAB) isolated from fermented foods with probiotic potential were evaluated for various tests, including exopolysaccharide production, antibiotic susceptibility, acid and bile tolerance, antibacterial activity,