-
The centrosome - diverse functions in fertilization and development across species. J. Cell Sci. (IF 4.0) Pub Date : 2023-12-01 Abrar Aljiboury,Heidi Hehnly
The centrosome is a non-membrane-bound organelle that is conserved across most animal cells and serves various functions throughout the cell cycle. In dividing cells, the centrosome is known as the spindle pole and nucleates a robust microtubule spindle to separate genetic material equally into two daughter cells. In non-dividing cells, the mother centriole, a substructure of the centrosome, matures
-
Crb3 is required to organize the apical domain of multiciliated cells. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-24 Céline Burcklé,Juliette Raitière,Grégoire Michaux,Laurent Kodjabachian,André Le Bivic
Cell shape changes mainly rely on the remodeling of the actin cytoskeleton. Multiciliated cells (MCCs) of the mucociliary epidermis of Xenopus laevis embryos, as they mature, dramatically reshape their apical domain to grow cilia, in coordination with the underlying actin cytoskeleton. Crumbs (Crb) proteins are multifaceted transmembrane apical polarity proteins known to recruit actin linkers and promote
-
Expanding roles of BCL-2 proteins in apoptosis execution and beyond. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-23 Louise E King,Lisa Hohorst,Ana J García-Sáez
The proteins of the BCL-2 family are known as key regulators of apoptosis, with interactions between family members determining permeabilisation of the mitochondrial outer membrane (MOM) and subsequent cell death. However, the exact mechanism through which they form the apoptotic pore responsible for MOM permeabilisation (MOMP), the structure and specific components of this pore, and what roles BCL-2
-
AP-1 signaling modulates cardiac fibroblast stress responses. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-23 Alexander J Whitehead,Hamza Atcha,James D Hocker,Bing Ren,Adam J Engler
Matrix remodeling outcomes largely dictate patient survival post-myocardial infarction. Moreover, human-restricted noncoding regulatory elements have been shown to worsen fibrosis, but their mechanism of action remains elusive. Here, we demonstrate using induced pluripotent stem cell derived cardiac fibroblasts (iCFs) that inflammatory ligands abundant in the remodeling heart after infarction activate
-
Cholesterol is required for activity-dependent synaptic growth. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-23 Amber Shaheen,Claire L Richter Gorey,Adam Sghaier,Jeffrey S Dason
Changes in cholesterol content of neuronal membranes occur during development and brain aging. Little is known about whether synaptic activity regulates cholesterol levels in neuronal membranes and whether these changes affect neuronal development and function. We generated transgenic flies that express the cholesterol-binding D4H domain of perfringolysin O toxin and found increased levels of cholesterol
-
Cellular responses to compound stress induced by atmospheric-pressure plasma in fission yeast. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-22 Yoko Otsubo,Akira Yamashita,Yuhei Goto,Keiichiro Sakai,Tetsushi Iida,Shinji Yoshimura,Katsuki Johzuka
The stress response is one of the most fundamental cellular processes. Although the molecular mechanisms underlying responses to a single stressor have been extensively studied, cellular responses to multiple stresses remain largely unknown. Here, we characterized fission yeast cellular responses to a novel stress inducer, non-thermal atmospheric-pressure plasma. Plasma irradiation generates ultraviolet
-
Cell jamming in a collagen-based interface assay: tuning by collagen density and proteolysis. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-21 Lianne Beunk,Nan Wen,Sjoerd van Helvert,Bram Bekker,Lars Ran,Ross Kang,Tom Paulat,Simon Syga,Andreas Deutsch,Peter Friedl,Katarina Wolf
Tumor cell invasion into heterogenous interstitial tissues consisting of network-, channel- or rift-like architecture involves both matrix metalloproteinase (MMP)-mediated tissue remodeling and cell-shape adaptation to tissue geometry. 3D models composed of either porous or linearly aligned architectures have added to the understanding of physical spacing principles on migration efficacy, however,
-
Identification of distinct active pools of yeast serine palmitoyltransferase in sub-compartments of the ER. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-20 Bianca M Esch,Stefan Walter,Oliver Schmidt,Florian Fröhlich
Sphingolipids (SP) are one of the three major lipid classes in eukaryotic cells and serve as structural components of the plasma membrane. The rate-limiting step in SP biosynthesis is catalyzed by the serine palmitoyltransferase (SPT). In yeast, SPT is negatively regulated by the two proteins, Orm1 and Orm2. Regulating SPT activity enables cells to adapt SP metabolism to changing environmental conditions
-
The epithelial polarity axis controls the resting membrane potential and Cl- co-transport in breast glandular structures. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-09 Albert K Urazaev,Lei Wang,Yunfeng Bai,Hibret A Adissu,Sophie A Lelièvre
The membrane potential (MP) controls cell homeostasis by directing molecule transport and gene expression. How the MP is set upon epithelial differentiation is unknown. Given that tissue architecture also controls homeostasis, we investigated the relationship between basoapical polarity and resting MP in three-dimensional culture of the HMT-3522 breast cancer progression. A microelectrode technique
-
30 years of nanobodies - an ongoing success story of small binders in biological research. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-08 Desiree I Frecot,Theresa Froehlich,Ulrich Rothbauer
A milestone in the field of recombinant binding molecules was achieved 30 years ago with the discovery of single-domain antibodies from which antigen-binding variable domains, better known as nanobodies (Nbs), can be derived. Being only one tenth the size of conventional antibodies, Nbs feature high affinity and specificity, while being highly stable and soluble. In addition, they display accessibility
-
Palmitate-induced insulin resistance causes actin filament stiffness and GLUT4 mis-sorting without altered Akt signalling. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-08 Victoria L Tokarz,Sivakami Mylvaganam,Amira Klip
Skeletal muscle insulin resistance, a major contributor to type 2 diabetes, is linked to the consumption of saturated fats. This insulin resistance arises from failure of insulin-induced translocation of glucose transporter type 4 (GLUT4; also known as SLC2A4) to the plasma membrane to facilitate glucose uptake into muscle. The mechanisms of defective GLUT4 translocation are poorly understood, limiting
-
Separation-of-function MCPH-associated mutations in CPAP affect centriole number and length. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-06 Sonal Jaiswal,Srishti Sanghi,Priyanka Singh
Centrioles are microtubule-based cylindrical ultrastructures characterized by their definite size and robustness. The molecular capping protein, CPAP (also known as CENPJ) engages its N-terminal region with the centriole microtubules to regulate their length. Nevertheless, the conserved C-terminal glycine-rich G-box of CPAP, which interacts with the centriole inner cartwheel protein STIL, is frequently
-
The antiproliferative effect of FGF2 in K-Ras-driven tumor cells involves modulation of rRNA and the nucleolus. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-30 Francisca N de Luna Vitorino,Michaella J Levy,Rosangela A Mansano Wailemann,Mariana Lopes,Mariana Loterio Silva,Mihaela E Sardiu,Benjamin A Garcia,Maria Cristina Machado Motta,Carla Columbano Oliveira,Hugo Aguirre Armelin,Laurence A Florens,Michael P Washburn,Julia Pinheiro Chagas da Cunha
The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response to stress signals. Despite being a growth factor, FGF2 has antiproliferative and tumor-suppressive functions in some cellular contexts. In this work, we investigated how the antiproliferative effect of FGF2 modulates chromatin-, nucleolus- and rDNA-associated proteins. The chromatin and nucleolar proteome
-
Cellular and molecular functions of SETD2 in the central nervous system. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-03 Benjamin Mitchell,Stefan Thor,Michael Piper
The covalent modification of histones is critical for many biological functions in mammals, including gene regulation and chromatin structure. Posttranslational histone modifications are added and removed by specialised 'writer' and 'eraser' enzymes, respectively. One such writer protein implicated in a wide range of cellular processes is SET domain-containing 2 (SETD2), a histone methyltransferase
-
Human sperm rotate with a conserved direction during free swimming in four dimensions. J. Cell Sci. (IF 4.0) Pub Date : 2023-11-29 Gabriel Corkidi,Fernando Montoya,Ana L González-Cota,Paul Hernández-Herrera,Neil C Bruce,Hermes Bloomfield-Gadêlha,Alberto Darszon
Head rotation in human spermatozoa is essential for different swimming modes and fertilisation, as it links the molecular workings of the flagellar beat with sperm motion in three-dimensional (3D) space over time. Determining the direction of head rotation has been hindered by the symmetry and translucent nature of the sperm head, and by the fast 3D motion driven by the helical flagellar beat. Analysis
-
The SUN-like protein TgSLP1 is essential for nuclear division in the apicomplexan parasite Toxoplasma gondii. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-30 Mirjam Wagner,Yuan Song,Elena Jiménez-Ruiz,Sonja Härtle,Markus Meissner
Connections between the nucleus and the cytoskeleton are important for positioning and division of the nucleus. In most eukaryotes, the linker of nucleoskeleton and cytoskeleton (LINC) complex spans the outer and inner nuclear membranes and connects the nucleus to the cytoskeleton. In opisthokonts, it is composed of Klarsicht, ANC-1 and Syne homology (KASH) domain proteins and Sad1 and UNC-84 (SUN)
-
Transcription inhibition suppresses nuclear blebbing and rupture independently of nuclear rigidity. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-27 Isabel K Berg,Marilena L Currey,Sarthak Gupta,Yasmin Berrada,Bao V Nguyen,Mai Pho,Alison E Patteson,J M Schwarz,Edward J Banigan,Andrew D Stephens
Chromatin plays an essential role in the nuclear mechanical response and determining nuclear shape, which maintain nuclear compartmentalization and function. However, major genomic functions, such as transcription activity, might also impact cell nuclear shape via blebbing and rupture through their effects on chromatin structure and dynamics. To test this idea, we inhibited transcription with several
-
Phosphorylation of axin within biomolecular condensates counteracts its tankyrase-mediated degradation. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-27 Katharina Klement,Martina Brückner,Dominic B Bernkopf
Axin (also known as AXIN1) is a central negative regulator of the proto-oncogenic Wnt/β-catenin signaling pathway, as axin condensates provide a scaffold for the assembly of a multiprotein complex degrading β-catenin. Axin, in turn, is degraded through tankyrase. Consequently, tankyrase small-molecule inhibitors block Wnt signaling by stabilizing axin, revealing potential for cancer therapy. Here,
-
Characterisation of TbSmee1 suggests endocytosis allows surface-bound cargo to enter the trypanosome flagellar pocket. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-26 Daja Schichler,Antonia Konle,Eva-Maria Spath,Sina Riegler,Alexandra Klein,Anna Seleznev,Sisco Jung,Timothy Wuppermann,Noah Wetterich,Alyssa Borges,Elisabeth Meyer-Natus,Katharina Havlicek,Sonia Pérez Cabrera,Korbinian Niedermüller,Sara Sajko,Maximilian Dohn,Xenia Malzer,Emily Riemer,Tuguldur Tumurbaatar,Kristina Djinovic-Carugo,Gang Dong,Christian J Janzen,Brooke Morriswood
All endocytosis and exocytosis in the African trypanosome Trypanosoma brucei occurs at a single subdomain of the plasma membrane. This subdomain, the flagellar pocket, is a small vase-shaped invagination containing the root of the single flagellum of the cell. Several cytoskeleton-associated multiprotein complexes are coiled around the neck of the flagellar pocket on its cytoplasmic face. One of these
-
Upregulation of fibronectin and its integrin receptors - an adaptation to isolation stress that facilitates tumor initiation. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-23 Chengsheng Wu,Sara M Weis,David A Cheresh
Tumor initiation at either primary or metastatic sites is an inefficient process in which tumor cells must fulfill a series of conditions. One critical condition involves the ability of individual tumor-initiating cells to overcome 'isolation stress', enabling them to survive within harsh isolating microenvironments that can feature nutrient stress, hypoxia, oxidative stress and the absence of a proper
-
Time-resolved proximity biotinylation implicates a porin protein in export of transmembrane malaria parasite effectors. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-18 David Anaguano,Watcharatip Dedkhad,Carrie F Brooks,David W Cobb,Vasant Muralidharan
The malaria-causing parasite, Plasmodium falciparum completely remodels its host red blood cell (RBC) through the export of several hundred parasite proteins, including transmembrane proteins, across multiple membranes to the RBC. However, the process by which these exported membrane proteins are extracted from the parasite plasma membrane for export remains unknown. To address this question, we fused
-
Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-18 Teresa Vitali,Rosa Sanchez-Alvarez,Tomasz M Witkos,Ioannis Bantounas,Marie F A Cutiongco,Michal Dudek,Guanhua Yan,Alexander A Mironov,Joe Swift,Martin Lowe
The Golgi complex comprises a connected ribbon of stacked cisternal membranes localized to the perinuclear region in most vertebrate cells. The position and morphology of this organelle depends upon interactions with microtubules and the actin cytoskeleton. In contrast, we know relatively little about the relationship of the Golgi complex with intermediate filaments (IFs). In this study, we show that
-
Atypical MAP kinases - new insights and directions from amoeba. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-16 Jeffrey A Hadwiger,Ramee G Aranda,Saher Fatima
Mitogen-activated protein kinases (MAPKs) have been the focus of many studies over the past several decades, but the understanding of one subgroup of MAPKs, orthologs of MAPK15, known as atypical MAPKs, has lagged behind others. In most organisms, specific activating signals or downstream responses of atypical MAPK signaling pathways have not yet been identified even though these MAPKs are associated
-
Epithelial mechanics are maintained by inhibiting cell fusion with age in Drosophila. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-16 Ari S Dehn,Levi Duhaime,Navdeep Gogna,Patsy M Nishina,Kristina Kelley,Vicki P Losick
A characteristic of normal aging and age-related diseases is the remodeling of the cellular organization of a tissue through polyploid cell growth. Polyploidy arises from an increase in nuclear ploidy or the number of nuclei per cell. However, it is not known whether age-induced polyploidy is an adaption to stressors or a precursor to degeneration. Here, we find that abdominal epithelium of the adult
-
Key role for Rac in the early transcriptional response to extracellular matrix stiffness and stiffness-dependent repression of ATF3. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-12 Irène Dang,Joseph A Brazzo,Yongho Bae,Richard K Assoian
The Rho family GTPases Rac and Rho play critical roles in transmitting mechanical information contained within the extracellular matrix (ECM) to the cell. Rac and Rho have well-described roles in regulating stiffness-dependent actin remodeling, proliferation and motility. However, much less is known about the relative roles of these GTPases in stiffness-dependent transcription, particularly at the
-
A molecular landscape of quiescence and proliferation highlights the role of Pten in mammary gland acinogenesis. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-11 Rebeka Tomasin,Ana Maria Rodrigues,Antonio Carlos Manucci,Alexandre Bruni-Cardoso
Cell context is key for cell state. Using physiologically relevant models of laminin-rich extracellular matrix (lrECM) induction of mammary epithelial cell quiescence and differentiation, we provide a landscape of the key molecules for the proliferation-quiescence decision, identifying multiple layers of regulation at the mRNA and protein levels. Quiescence occurred despite activity of Fak (also known
-
Loss of ATG4B and ATG4A results in two-stage cell cycle defects in pancreatic ductal adenocarcinoma cells. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-11 Paalini Sathiyaseelan,Suganthi Chittaranjan,Steve E Kalloger,Jennifer Chan,Nancy E Go,Mario A Jardon,Cally J Ho,Theodore Hui,Jing Xu,Christine Chow,Dongxia Gao,Fraser D Johnson,William W Lockwood,Gregg B Morin,Daniel J Renouf,David F Schaeffer,Sharon M Gorski
Pancreatic ductal adenocarcinoma (PDAC) exhibits elevated levels of autophagy, which promote tumor progression and treatment resistance. ATG4B is an autophagy-related cysteine protease under consideration as a potential therapeutic target, but it is largely unexplored in PDAC. Here, we investigated the clinical and functional relevance of ATG4B expression in PDAC. Using two PDAC patient cohorts, we
-
Reconstituted in vitro systems to reveal the roles and functions of septins. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-10 Koyomi Nakazawa,Brieuc Chauvin,Stéphanie Mangenot,Aurélie Bertin
Septins are essential cytoskeletal proteins involved in key cellular processes and have also been implicated in diseases from cancers to neurodegenerative pathologies. However, they have not been as thoroughly studied as other cytoskeletal proteins. In vivo, septins interact with other cytoskeletal proteins and with the inner plasma membrane. Hence, bottom-up in vitro cell-free assays are well suited
-
'Chip'-ing away at morphogenesis - application of organ-on-chip technologies to study tissue morphogenesis. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-05 Matthew J White,Tania Singh,Eric Wang,Quinton Smith,Matthew L Kutys
Emergent cell behaviors that drive tissue morphogenesis are the integrated product of instructions from gene regulatory networks, mechanics and signals from the local tissue microenvironment. How these discrete inputs intersect to coordinate diverse morphogenic events is a critical area of interest. Organ-on-chip technology has revolutionized the ability to construct and manipulate miniaturized human
-
Compromised junctional integrity phenocopies age-dependent renal dysfunction in Drosophila Snakeskin mutants. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-05 Anthony J Dornan,Kenneth V Halberg,Liesa-Kristin Beuter,Shireen-Anne Davies,Julian A T Dow
Transporting epithelia provide a protective barrier against pathogenic insults while allowing the controlled exchange of ions, solutes and water with the external environment. In invertebrates, these functions depend on formation and maintenance of 'tight' septate junctions (SJs). However, the mechanism by which SJs affect transport competence and tissue homeostasis, and how these are modulated by
-
Global and local functions of the Fused kinase ortholog CdaH in intracellular patterning in Tetrahymena. J. Cell Sci. (IF 4.0) Pub Date : 2023-10-04 Chinkyu Lee,Wolfgang Maier,Yu-Yang Jiang,Kentaro Nakano,Karl F Lechtreck,Jacek Gaertig
Ciliates assemble numerous microtubular structures into complex cortical patterns. During ciliate division, the pattern is duplicated by intracellular segmentation that produces a tandem of daughter cells. In Tetrahymena thermophila, the induction and positioning of the division boundary involves two mutually antagonistic factors: posterior CdaA (cyclin E) and anterior CdaI (Hippo kinase). Here, we
-
Surf4 collaborates with derlin-2 and derlin-1 to mediate cyclooxygenase-2 translocation to the cytosol for degradation. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-28 Shu-Fen Chen,Chun-Hu Wu,Yen-Ming Lee,Kabik Tam,Jun-Yang Liou,Song-Kun Shyue
Derlin family members participate in the retrotranslocation of endoplasmic reticulum (ER) lumen proteins to the cytosol for ER-associated degradation (ERAD); however, the proteins facilitating this retrotranslocation remain to be explored. Using CRISPR library screening, we have found that derlin-2 and surfeit locus protein 4 (Surf4) are candidates to facilitate degradation of cyclooxygenase-2 (COX-2
-
Live-imaging studies reveal how microclots and the associated inflammatory response enhance cancer cell extravasation. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-28 Juma Ward,Paul Martin
Previous clinical studies and work in mouse models have indicated that platelets and microclots might enable the recruitment of immune cells to the pre-metastatic cancer niche, leading to efficacious extravasation of cancer cells through the vessel wall. Here, we investigated the interaction between platelets, endothelial cells, inflammatory cells, and engrafted human and zebrafish cancer cells by
-
Paxillin regulates liver fibrosis via actin polymerization and ERK activation in hepatic stellate cells. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-28 Nour Hijazi,Zengdun Shi,Don C Rockey
Liver injury leads to fibrosis and cirrhosis. The primary mechanism underlying the fibrogenic response is the activation of hepatic stellate cells (HSCs), which are 'quiescent' in normal liver but become 'activated' after injury by transdifferentiating into extracellular matrix (ECM)-secreting myofibroblasts. Given that integrins are important in HSC activation and fibrogenesis, we hypothesized that
-
Response of cells and tissues to shear stress. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-25 Jaime A Espina,Marilia H Cordeiro,Milan Milivojevic,Ivana Pajić-Lijaković,Elias H Barriga
Shear stress is essential for normal physiology and malignancy. Common physiological processes - such as blood flow, particle flow in the gut, or contact between migratory cell clusters and their substrate - produce shear stress that can have an impact on the behavior of different tissues. In addition, shear stress has roles in processes of biomedical interest, such as wound healing, cancer and fibrosis
-
Expression of Concern: Estrogen receptor mediates a distinct mitochondrial unfolded protein response. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-25 Luena Papa,Doris Germain
-
Shock drives a STAT3 and JunB-mediated coordinated transcriptional and DNA methylation response in the endothelium. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-25 Ramon Bossardi Ramos,Nina Martino,Dareen Chuy,Shuhan Lu,Mei Xing G Zuo,Uma Balasubramanian,Iria Di John Portela,Peter A Vincent,Alejandro P Adam
Endothelial dysfunction is a crucial factor in promoting organ failure during septic shock. However, the underlying mechanisms are unknown. Here, we show that kidney injury after lipopolysaccharide (LPS) insult leads to strong endothelial transcriptional and epigenetic responses. Furthermore, SOCS3 loss leads to an aggravation of the responses, demonstrating a causal role for the STAT3-SOCS3 signaling
-
Chloroplast protein translocation pathways and ubiquitin-dependent regulation at a glance. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-21 Sreedhar Nellaepalli,Anne Sophie Lau,R Paul Jarvis
Chloroplasts conduct photosynthesis and numerous metabolic and signalling processes that enable plant growth and development. Most of the ∼3000 proteins in chloroplasts are nucleus encoded and must be imported from the cytosol. Thus, the protein import machinery of the organelle (the TOC-TIC apparatus) is of fundamental importance for chloroplast biogenesis and operation. Cytosolic factors target chloroplast
-
Schwann cells are axo-protective after injury irrespective of myelination status in mouse Schwann cell-neuron cocultures. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-20 Clara Mutschler,Shaline V Fazal,Nathalie Schumacher,Andrea Loreto,Michael P Coleman,Peter Arthur-Farraj
Myelinating Schwann cell (SC)-dorsal root ganglion (DRG) neuron cocultures are an important technique for understanding cell-cell signalling and interactions during peripheral nervous system (PNS) myelination, injury, and regeneration. Although methods using rat SCs and neurons or mouse DRG explants are commonplace, there are no established protocols for compartmentalised myelinating cocultures with
-
ILT4 reprograms glucose metabolism to promote tumor progression in triple-negative breast cancer. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-18 Haiqin Zhang,Aiqin Gao,Qiaohong Liu,Fang Zhang,Shuyun Wang,Xiaozheng Chen,Wenjing Shi,Ye Zhang,Qian Liu,Yan Zheng,Yuping Sun
Triple-negative breast cancer (TNBC) is the most aggressive and poorly treated subtype of breast cancer. Identifying novel drivers and mechanisms for tumor progression is essential for precise targeted therapy of TNBC. Immunoglobulin-like transcript 4 (ILT4; also known as LILRB2) is a classic myeloid suppressor for their activation and immune response. Our recent results found that ILT4 is also highly
-
Y-27632 acts beyond ROCK inhibition to maintain epidermal stem-like cells in culture. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-12 Travis A Witkowski,Bin Li,Jason G Andersen,Bhavna Kumar,Edmund A Mroz,James W Rocco
Conditional reprogramming is a cell culture technique that effectively immortalizes epithelial cells with normal genotypes by renewing epidermal stem cells. Y-27632, a compound that promotes conditional reprogramming through an unknown mechanism, was developed to inhibit the two Rho-associated kinase (ROCK) isoforms. We used human foreskin keratinocytes (HFKs) to study the role of Y-27632 in conditional
-
Leucine-rich repeat kinase 2 at a glance. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-12 Christiane Zhu,Susanne Herbst,Patrick A Lewis
Leucine-rich repeat kinase 2 (LRRK2) is a multidomain scaffolding protein with dual guanosine triphosphatase (GTPase) and kinase enzymatic activities, providing this protein with the capacity to regulate a multitude of signalling pathways and act as a key mediator of diverse cellular processes. Much of the interest in LRRK2 derives from mutations in the LRRK2 gene being the most common genetic cause
-
p53 amyloid pathology is correlated with higher cancer grade irrespective of the mutant or wild-type form. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-08 Shinjinee Sengupta,Namrata Singh,Ajoy Paul,Debalina Datta,Debdeep Chatterjee,Semanti Mukherjee,Laxmikant Gadhe,Jyoti Devi,Yeshwanth Mahesh,Mohit Kumar Jolly,Samir K Maji
p53 (also known as TP53) mutation and amyloid formation are long associated with cancer pathogenesis; however, the direct demonstration of the link between p53 amyloid load and cancer progression is lacking. Using multi-disciplinary techniques and 59 tissues (53 oral and stomach cancer tumor tissue samples from Indian individuals with cancer and six non-cancer oral and stomach tissue samples), we showed
-
Actomyosin-dependent cell contractility orchestrates Zika virus infection. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-08 Xinyi Huang,Yifan Xing,Yanqin Cui,Baohua Ji,Binbin Ding,Jin Zhong,Yaming Jiu
Emerging pathogen infections, such as Zika virus (ZIKV), pose an increasing threat to human health, but the role of mechanobiological attributes of host cells during ZIKV infection is largely unknown. Here, we reveal that ZIKV infection leads to increased contractility of host cells. Importantly, we investigated whether host cell contractility contributes to ZIKV infection efficacy, from both the intracellular
-
Mitochondrial phospholipid metabolism in health and disease. J. Cell Sci. (IF 4.0) Pub Date : 2023-09-01 Alaumy Joshi,Travis H Richard,Vishal M Gohil
Studies of rare human genetic disorders of mitochondrial phospholipid metabolism have highlighted the crucial role that membrane phospholipids play in mitochondrial bioenergetics and human health. The phospholipid composition of mitochondrial membranes is highly conserved from yeast to humans, with each class of phospholipid performing a specific function in the assembly and activity of various mitochondrial
-
A WNT4- and DKK3-driven canonical to noncanonical Wnt signaling switch controls multiciliogenesis. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-29 Riley A Cooney,Maxwell L Saal,Kara P Geraci,Caitlin Maynard,Ondine Cleaver,Oanh N Hoang,Todd T Moore,Rosa F Hwang,Jeffrey D Axelrod,Eszter K Vladar
Multiciliated cells contain hundreds of cilia whose directional movement powers the mucociliary clearance of the airways, a vital host defense mechanism. Multiciliated cell specification requires canonical Wnt signaling, which then must be turned off. Next, ciliogenesis and polarized ciliary orientation are regulated by noncanonical Wnt/planar cell polarity (Wnt/PCP) signaling. The mechanistic relationship
-
Knockout of all ErbB-family genes delineates their roles in proliferation, survival and migration. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-21 Kimiya Matsuda,Daiki Hirayama,Naoya Hino,Sota Kuno,Asako Sakaue-Sawano,Atsushi Miyawaki,Michiyuki Matsuda,Kenta Terai
The ErbB-family receptors play pivotal roles in the proliferation, migration and survival of epithelial cells. Because our knowledge on the ErbB-family receptors has been largely obtained by the exogenous application of their ligands, it remains unknown to what extent each of the ErbB members contributes to these outputs. We here knocked out each ErbB gene, various combinations of ErbB genes or all
-
Actin cytoskeletal dynamics do not impose an energy drain on growth cone bioenergetics. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-18 Sabrina M Holland,Gianluca Gallo
The regulation of the intracellular level of ATP is a fundamental aspect of bioenergetics. Actin cytoskeletal dynamics have been reported to be an energetic drain in developing neurons and platelets. We addressed the role of actin dynamics in primary embryonic chicken neurons using luciferase assays, and by measurement of the ATP/ADP ratio using the ratiometric reporter PercevalHR and the ATP level
-
α- and β-tubulin C-terminal tails with distinct modifications are crucial for ciliary motility and assembly. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-17 Tomohiro Kubo,Yuma Tani,Haru-Aki Yanagisawa,Masahide Kikkawa,Toshiyuki Oda
α- and β-tubulin have an unstructured glutamate-rich region at their C-terminal tails (CTTs). The function of this region in cilia and flagella is still unclear, except that glutamates in CTTs act as the sites for post-translational modifications that affect ciliary motility. The unicellular alga Chlamydomonas possesses only two α-tubulin and two β-tubulin genes, each pair encoding an identical protein
-
Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-17 Heather D Edgerton,Soumya Mukherjee,Marnie Johansson,Jeff Bachant,Melissa K Gardner,Duncan J Clarke
Accurate genome segregation in mitosis requires that all chromosomes are bioriented on the spindle. Cells monitor biorientation by sensing tension across sister centromeres. Chromosomes that are not bioriented have low centromere tension, which allows Aurora B (yeast Ipl1) to perform error correction that locally loosens kinetochore-microtubule attachments to allow detachment of microtubules and fresh
-
The plasma membrane of focal adhesions has a high content of cholesterol and phosphatidylcholine with saturated acyl chains. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-17 Hiroshi Tachibana,Kodai Minoura,Tomohiro Omachi,Kohjiro Nagao,Takafumi Ichikawa,Yasuhisa Kimura,Nozomu Kono,Yuta Shimanaka,Hiroyuki Arai,Kazumitsu Ueda,Noriyuki Kioka
Cellular functions, such as differentiation and migration, are regulated by the extracellular microenvironment, including the extracellular matrix (ECM). Cells adhere to ECM through focal adhesions (FAs) and sense the surrounding microenvironments. Although FA proteins have been actively investigated, little is known about the lipids in the plasma membrane at FAs. In this study, we examine the lipid
-
Post-translational modifications of ATG8 proteins - an emerging mechanism of autophagy control. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-15 Jose L Nieto-Torres,Sviatlana Zaretski,Tianhui Liu,Peter D Adams,Malene Hansen
Autophagy is a recycling mechanism involved in cellular homeostasis with key implications for health and disease. The conjugation of the ATG8 family proteins, which includes LC3B (also known as MAP1LC3B), to autophagosome membranes, constitutes a hallmark of the canonical autophagy process. After ATG8 proteins are conjugated to the autophagosome membranes via lipidation, they orchestrate a plethora
-
Glucose oxidation drives trunk neural crest cell development and fate. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-15 Nioosha Nekooie Marnany,Redouane Fodil,Sophie Féréol,Alwyn Dady,Marine Depp,Frederic Relaix,Roberto Motterlini,Roberta Foresti,Jean-Loup Duband,Sylvie Dufour
Bioenergetic metabolism is a key regulator of cellular function and signaling, but how it can instruct the behavior of cells and their fate during embryonic development remains largely unknown. Here, we investigated the role of glucose metabolism in the development of avian trunk neural crest cells (NCCs), a migratory stem cell population of the vertebrate embryo. We uncovered that trunk NCCs display
-
The role of metabolism in cellular quiescence. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-15 Yipeng Du,Parul Gupta,Shenlu Qin,Matthew Sieber
Cellular quiescence is a dormant, non-dividing cell state characterized by significant shifts in physiology and metabolism. Quiescence plays essential roles in a wide variety of biological processes, ranging from microbial sporulation to human reproduction and wound repair. Moreover, when the regulation of quiescence is disrupted, it can drive cancer growth and compromise tissue regeneration after
-
The endosomal system of primary human vascular endothelial cells and albumin-FcRn trafficking. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-11 Andreas Pannek,Janine Becker-Gotot,Steven K Dower,Anne M Verhagen,Paul A Gleeson
Human serum albumin (HSA) has a long circulatory half-life owing, in part, to interaction with the neonatal Fc receptor (FcRn or FCGRT) in acidic endosomes and recycling of internalised albumin. Vascular endothelial and innate immune cells are considered the most relevant cells for FcRn-mediated albumin homeostasis in vivo. However, little is known about endocytic trafficking of FcRn-albumin complexes
-
ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-09 Juan Wang,Lang-Fan Zheng,Su Ren,Dong-Lin Li,Chen Chen,Hui-Hui Sun,Li-Ying Liu,Huiling Guo,Tong-Jin Zhao
Protein palmitoylation is a post-translational lipid modification of proteins. Accumulating evidence reveals that palmitoylation functions as a sorting signal to direct proteins to destinations; however, the sorting mechanism remains largely unknown. Here, we show that ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane (PM). Through shRNA screening,
-
Import and quality control of peroxisomal proteins. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-08 Markus Rudowitz,Ralf Erdmann
Peroxisomes are involved in a multitude of metabolic and catabolic pathways, as well as the innate immune system. Their dysfunction is linked to severe peroxisome-specific diseases, as well as cancer and neurodegenerative diseases. To ensure the ability of peroxisomes to fulfill their many roles in the organism, more than 100 different proteins are post-translationally imported into the peroxisomal
-
ZnUMBA - a live imaging method to detect local barrier breaches. J. Cell Sci. (IF 4.0) Pub Date : 2023-08-08 Tomohito Higashi,Rachel E Stephenson,Cornelia Schwayer,Karla Huljev,Atsuko Y Higashi,Carl-Philipp Heisenberg,Hideki Chiba,Ann L Miller
Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods measure changes in global barrier function, they lack the sensitivity needed to detect local or transient barrier breaches, and they do not reveal the
-
Mechanotransduction through hemidesmosomes during aging and longevity. J. Cell Sci. (IF 4.0) Pub Date : 2023-07-31 Collin Y Ewald,Alexander Nyström
Hemidesmosomes are structural protein complexes localized at the interface of tissues with high mechanical demand and shear forces. Beyond tissue anchoring, hemidesmosomes have emerged as force-modulating structures important for translating mechanical cues into biochemical and transcriptional adaptation (i.e. mechanotransduction) across tissues. Here, we discuss the recent insights into the roles
-
A minimal cell model for lamellipodia-based cellular dynamics and migration. J. Cell Sci. (IF 4.0) Pub Date : 2023-07-27 Raj Kumar Sadhu,Aleš Iglič,Nir S Gov
One ubiquitous cellular structure for performing various tasks, such as spreading and migration over external surfaces, is the sheet-like protrusion called a lamellipodium, which propels the leading edge of the cell. Despite the detailed knowledge about the many components of this cellular structure, it is not yet fully understood how these components self-organize spatiotemporally to form lamellipodia