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Exploring the effect of Clostridium butyricum on lung injury associated with acute pancreatitis in mice by combined 16S rRNA and metabolomics analysis Anaerobe (IF 2.3) Pub Date : 2024-04-16 Jiaxin Liu, Biyan Wen, Yaoxing Huang, Guiqing Deng, Qingqing Yan, Lin Jia
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Novel transcriptional regulator OxtR1 regulates potential ferrodoxin in response to oxygen stress in Treponema denticola Anaerobe (IF 2.3) Pub Date : 2024-04-16 Yumi Numata, Yuichiro Kikuchi, Toru Sato, Kazuko Okamoto-Shibayama, Yutaro Ando, Yuri Miyai-Murai, Eitoyo Kokubu, Kazuyuki Ishihara
has been strongly implicated in the pathogenesis of chronic periodontitis. Previously, we reported that the potential transcriptional regulator TDE_0259 ( is upregulated in the bacteriocin ABC transporter gene-deficient mutant. OxtR1 may regulate genes to adapt to environmental conditions during colonization; however, the exact role of the gene in has not been reported. Therefore, we investigated its
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Optimization of CO fermentation by Clostridium carboxidivorans in batch reactors: Effects of the medium composition Anaerobe (IF 2.3) Pub Date : 2024-04-12 F. Lanzillo, S. Pisacane, F. Raganati, M.E. Russo, P. Salatino, A. Marzocchella
The objective of this study was to investigate the effects of medium composition on CO fermentation by . The focus was to reduce the medium cost preserving acceptable levels of solvent production. Yeast extract (YE) concentration was set in the range of 0–3 g/L. Different reducing agents were investigated, including cysteine-HCl 0.6 g/L, pure cysteine 0.6 g/L, sodium sulphide (Na2S) 0.6 g/L, cysteine-sodium
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Human fecal alpha-glucosidase activity and its relationship with gut microbiota profiles and early stages of intestinal mucosa damage Anaerobe (IF 2.3) Pub Date : 2024-04-12 Sergio Ruiz-Saavedra, Nuria Salazar, Adolfo Suárez, Ylenia Diaz, Carmen González del Rey, Sonia González, Clara G. de los Reyes-Gavilán
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RNA-based regulation in bacteria-phage interactions Anaerobe (IF 2.3) Pub Date : 2024-04-06 Marion Saunier, Louis-Charles Fortier, Olga Soutourina
Interactions of bacteria with their viruses named bacteriophages or phages shape the bacterial genome evolution and contribute to the diversity of phages. RNAs have emerged as key components of several anti-phage defense systems in bacteria including CRISPR-Cas, toxin-antitoxin and abortive infection. Frequent association with mobile genetic elements and interplay between different anti-phage defense
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The small acid-soluble proteins of spore-forming organisms: Similarities and differences in function Anaerobe (IF 2.3) Pub Date : 2024-04-04 Hailee N. Nerber, Joseph A. Sorg
The small acid-soluble proteins are found in all endospore-forming organisms and are a major component of spores. Through their DNA binding capabilities, the SASPs shield the DNA from outside insults (e.g., UV and genotoxic chemicals). The absence of the major SASPs results in spores with reduced viability when exposed to UV light and, in at least one case, the inability to complete sporulation. While
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The impact of existing total anti-toxin B IgG immunity in outcomes of recurrent Clostridioides difficile infection Anaerobe (IF 2.3) Pub Date : 2024-03-28 Isaura Rigo, Mary K. Young, Mayuresh M. Abhyankar, Feifan Xu, Girija Ramakrishnan, Farha Naz, Gregory R. Madden, William A. Petri
Late anti-toxin-B humoral immunity acquired after treatment is important for preventing recurrent infection. We prospectively-measured anti-toxin-B IgG and neutralization titers at diagnosis as potential early predictors of recurrence. High anti-toxin-B-IgG/neutralizing antibodies were associated with short-lasting protection within 6-weeks, however, no difference in recurrence risk was observed by
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Approaching toxigenic Clostridia from a one health perspective Anaerobe (IF 2.3) Pub Date : 2024-03-27 Laura M. Cersosimo, Jay N. Worley, Lynn Bry
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Clostridioides difficile infection promotes gastrointestinal dysfunction in human and mice post-acute phase of the disease Anaerobe (IF 2.3) Pub Date : 2024-03-26 Deiziane V.S. Costa, Natalie Pham, Andrea V. Loureiro, Suemin E. Yang, Brian W. Behm, Cirle A. Warren
In the US, () infection (CDI) is the 8th leading cause of hospital readmission and 7th for mortality among all gastrointestinal (GI) disorders. Here, we investigated GI dysfunction post-CDI in humans and mice post-acute infection. From March 2020 to July 2021, we reviewed the clinical records of 67 patients referred to the UVA Complicated linic for fecal microbiota transplantation (FMT) eligibility
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Antimicrobial effects of cannabidiol on select agriculturally important Clostridia Anaerobe (IF 2.3) Pub Date : 2024-03-25 Jourdan E. Lakes, Jessica L. Ferrell, Mark A. Berhow, Michael D. Flythe
Amino acid-fermenting Clostridia have undesirable effects in agricultural systems, which can be mitigated by antibiotics, but resistance necessitates alternatives. Here, we demonstrate the efficacy of cannabidiol on growth and ammonia inhibition of five agriculturally relevant Clostridia: , spp., , , and .
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Hungatella hathewayi bacteremia due to acute appendicitis: A case report and a narrative review Anaerobe (IF 2.3) Pub Date : 2024-03-21 Elie Bou Sanayeh, Mark Tawfik, Marina Makram, Georges Khattar, Faris Qaqish, Sudeep Acharya, Janet Farag, Michel Chalhoub, Allison Glaser
species, including and , previously identified as part of the genus, are anaerobic bacteria primarily residing in the gut microbiome, with infrequent implications in human infections. This article presents the case of an 87-year-old Asian male admitted for a hyperosmolar hyperglycemic state with septic shock secondary to bacteremia originating from acute appendicitis. Remarkably, the bacterium was
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Fecal microbiota transplantation stimulates type 2 and tolerogenic immune responses in a mouse model Anaerobe (IF 2.3) Pub Date : 2024-03-21 G. Brett Moreau, Farha Naz, William A. Petri Jr.
infection (CDI) is the leading hospital-acquired infection in North America. While previous work on fecal microbiota transplantation (FMT), a highly effective treatment for CDI, has focused on colonization resistance mounted against by FMT-delivered commensals, the effects of FMT on host gene expression are relatively unexplored. This study aims to identify transcriptional changes associated with FMT
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Systemic neutrophil degranulation and emergency granulopoiesis in patients with Clostridioides difficile infection Anaerobe (IF 2.3) Pub Date : 2024-03-20 Girija Ramakrishnan, Mary K. Young, Uma Nayak, Isaura Rigo, Andrea S. Marrs, Carol A. Gilchrist, Brian W. Behm, Gregory R. Madden, William A. Petri Jr.
infection (CDI) is characterized by neutrophilia in blood, with a high leukocyte count accompanying severe infection. In this study, we characterized peripheral blood neutrophil activation and maturity in CDI by (i) developing a method to phenotype stored neutrophils for disease-related developmental alterations and (ii) assessing neutrophil-associated biomarkers. We stored fixed leukocytes from blood
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Increase of healthcare-onset Clostridioides difficile infection in adult population since SARS-CoV-2 pandemic: A retrospective cohort study in a tertiary care hospital from 2019 to 2022 Anaerobe (IF 2.3) Pub Date : 2024-02-28 Alfredo Maldonado-Barrueco, Francisco Moreno-Ramos, Beatriz Díaz-Pollán, Belén Loeches-Yagüe, Alicia Rico-Nieto, Julio García-Rodríguez, Guillermo Ruiz-Carrascoso
The aim was to assess the impact of the SARS-CoV-2 pandemic on the prevalence, relative incidence (RI), incidence density (ID), ratio of rate incidence (RRI), rate of incidence density (RID), and relative risks (RR) of healthcare-onset infection (HO-CDI) as well as its correlation with the antibiotic consumption. Demographic and analytical data of adult patients exhibiting diarrhoea and testing positive
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Low usefulness of reporting tonsillar PCR Ct-values in pharyngeal infections with Fusobacterium necrophorum Anaerobe (IF 2.3) Pub Date : 2024-02-16 David Nygren, Lisa Wasserstrom, Gustav Torisson, Karin Holm
Tonsillar PCR Ct-values were higher in participants with asymptomatic tonsillar carriage than patients with pharyngeal infections. However, Ct-values were not associated with severity of disease or predictive of development of complications and hence lacked clinical usefulness. The reporting of Ct-values in clinical samples is not recommended.
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Novel and rare β-lactamase genes of Bacteroides fragilis group species: Detection of the genes and characterization of their genetic backgrounds Anaerobe (IF 2.3) Pub Date : 2024-02-13 Bakhtiyar Mahmood, Károly Péter Sárvári, Laszló Orosz, Elisabeth Nagy, József Sóki
This study screened the prevalence of rare β-lactamase genes in group strains from clinical specimens and normal microbiota and examined the genetic properties of the strains carrying these genes. HGD1, OXA347, , , and were detected by real-time polymerase chain reaction in collections of strains from clinical (n = 406) and fecal (n = 184) samples. To examine the genetic backgrounds of the samples
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Microscopy methods for Clostridioides difficile Anaerobe (IF 2.3) Pub Date : 2024-02-08 Chenlin Hu, Kevin W. Garey
Microscopic technologies including light and fluorescent, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and cryo-electron microscopy have been widely utilized to visualize C at the molecular, cellular, community, and structural biology level. This comprehensive review summarizes the microscopy tools (fluorescent and reporter system) in their use to study different aspects
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The burden of Clostridioides difficile infections in South-East Asia and the Western Pacific: A narrative review Anaerobe (IF 2.3) Pub Date : 2024-02-07 Frederick J. Angulo, Canna Ghia, Mark A. Fletcher, Egemen Ozbilgili, Graciela del Carmen Morales
(formerly ) is well-documented in Europe and North America to be a common cause of healthcare-associated gastrointestinal tract infections. In contrast, infection (CDI) is infrequently reported in literature from Asia, which may reflect a lack of clinician awareness. We conducted a narrative review to better understand CDI burden in Asia. We searched the PubMed database for English language articles
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Polyphasic molecular approach to the characterization of methanogens in the saliva of Tunisian adults Anaerobe (IF 2.3) Pub Date : 2024-02-02 Fériel Bouzid, Imen Gtif, Salma Charfeddine, Leila Abid, Najla Kharrat
Methanogenic archaea are a minor component of human oral microbiota. Due to their relatively low abundance, the detection of these neglected microorganisms is challenging. This study concerns the presence of methanogens in salivary samples collected from Tunisian adults to evaluate their prevalence and burden using a polyphasic molecular approach. A total of 43 saliva samples were included. Metagenomic
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Bacteroides and related species: The keystone taxa of the human gut microbiota Anaerobe (IF 2.3) Pub Date : 2024-01-10 Jae Hyun Shin, Glenn Tillotson, Tiffany N. MacKenzie, Cirle A. Warren, Hannah M. Wexler, Ellie J.C. Goldstein
Microbial communities play a significant role in maintaining ecosystems in a healthy homeostasis. Presently, in the human gastrointestinal tract, there are certain taxonomic groups of importance, though there is no single species that plays a keystone role. Bacteroides spp. are known to be major players in the maintenance of eubiosis in the human gastrointestinal tract. Here we review the critical
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Clinical and microbiological characteristics of Ruminococcus gnavus bacteremia and intra-abdominal infection Anaerobe (IF 2.3) Pub Date : 2024-01-10 Naoki Watanabe, Tomohisa Watari, Yoshihito Otsuka, Naoto Hosokawa, Kazufumi Yamagata, Miyuki Fujioka
Objectives Ruminococcus gnavus is a rare human pathogen, and clinical data on R. gnavus infection are insufficient. This retrospective study aimed to investigate the clinical characteristics of R. gnavus infections. Methods This study included 13 cases of bacteremia and three cases of non-bacteremia infections caused by R. gnavus. We evaluated the patient data, infection source, clinical outcomes,
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Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins Anaerobe (IF 2.3) Pub Date : 2023-12-30 Guilherme Guerra Alves, Ronnie Antunes Assis, Victor Santos do Amarante, Carlos Augusto de Oliveira Júnior, Rodrigo Otávio Silveira Silva, Luiz Guilherme Dias Heneine, Francisco Carlos Faria Lobato
Objectives This study aimed to produce and purify Clostridium perfringens type C beta-toxin, sheep anti-beta toxin immunoglobulin G (IgG) and chicken immunoglobulin Y (IgY). Methods Two methods were used for beta-toxin purification: single-step metal affinity chromatography (MAC) using zinc as a chelator and ion exchange chromatography (IEX). The purified and inactivated beta-toxoids were then administered
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In vitro activity of delafloxacin against anaerobic bacteria compared with other antimicrobials Anaerobe (IF 2.3) Pub Date : 2023-12-23 Fernando Cobo, Ana Franco-Acosta, Lina Martín-Hita, Javier Rodríguez-Granger, Antonio Sampedro-Martínez, Juan Antonio Reguera-Márquez, José María Navarro-Marí
The aim of this study was to describe the in vitro activity of delafloxacin against 230 anaerobic isolates and compare it with the activity of other antimicrobials used against infections caused by anaerobic microorganisms. Minimal inhibitory concentrations (MICs) were lower for delafloxacin than for all other antibiotics tested with the exception of piperacillin-tazobactam and meropenem against Gram-positive
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Enhancing biogas generation from lignocellulosic biomass through biological pretreatment: Exploring the role of ruminant microbes and anaerobic fungi Anaerobe (IF 2.3) Pub Date : 2023-12-23 R. Tamilselvan, A. Immanuel Selwynraj
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TMT-based quantitative proteomics reveals the nutritional and stress resistance functions of anaerobic fungi in yak rumen during passage at different time intervals Anaerobe (IF 2.3) Pub Date : 2023-12-02 Jingrong Zhang, Yaqin Wei, Huizhen Qiu, Jiayi Han
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Evaluation of the additive effects of volatile fatty acids and moderate heat treatment for enhancing the inactivation of vegetative cells and spores of Clostridium perfringens by flow cytometry Anaerobe (IF 2.3) Pub Date : 2023-11-23 Cedric Delmon, Catherine Ouk, Magali Casellas, Audrey Prorot
Objectives Clostridium perfringens is a well-known spore-forming bacterium that can resist the environment. A mixture of volatile fatty acids or thermal treatments can interact with these bacteria. The aim of this study was to evaluate the effects of different volatile fatty acid concentrations and moderate heat treatment on Clostridium perfringens sporulation. Methods A pure culture of Clostridium
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Brain abscess caused by Porphyromonas gingivalis and Eubacterium nodatum mimicking acute stroke Anaerobe (IF 2.3) Pub Date : 2023-11-23 Domingo Fernández Vecilla, Mary Paz Roche Matheus, Mikel Urrutikoetxea Gutiérrez, Gotzon Iglesias Hidalgo, Cristina Aspichueta Vivanco, José Luis Díaz de Tuesta del Arco
We described a case of a 65-year-old man with a brain abscess caused by Porphyromonas gingivalis and Eubacterium nodatum. The patient presented right central facial nerve palsy, mutism and right hemiparesis at the examination. The patient underwent a left frontal craniotomy with evacuation of the brain abscess. Specimens were collected for microbiological analysis and intravenous treatment was started
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Clinical usefulness of anaerobic blood culture in pediatric patients with bacteremia Anaerobe (IF 2.3) Pub Date : 2023-11-20 Ga Young Noh, Yoon Soo Park, Si Hyun Kim, Sae Am Song, Jeong Hwan Shin
Objectives The purpose of this study is to evaluate the usefulness of anaerobic blood culture in pediatric patients by comparing the detection rate and distribution of bacteria between aerobic and anaerobic blood culture bottles. Methods A retrospective analysis was conducted on 11,664 blood cultures obtained from children under the age of 14 between January 2013 and June 2020. The positive rate of
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Antimicrobial susceptibility testing of anaerobic bacteria causing bacteremia: A 13-year (2010–2022) retrospective study in a tertiary hospital Anaerobe (IF 2.3) Pub Date : 2023-11-19 Jorge Ligero-López, Eduardo Rubio-Mora, Mario David Ruiz-Bastián, María Inmaculada Quiles-Melero, Juana Cacho-Calvo, Emilio Cendejas-Bueno
Infections from anaerobic microorganisms result from breached mucosal barriers, posing a significant mortality risk. A retrospective study at Hospital Universitario La Paz (Madrid) from 2010 to 2022 analyzed 491 (6.17 %) anaerobic bacteremia cases out of 7956 significant bacteremia cases among 171,833 blood culture requests. Bacteroides fragilis was the most frequently isolated species (28.3 %), followed
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Efficacy of bezlotoxumab to prevent recurrent Clostridioides difficile infection (CDI) in patients with multiple prior recurrent CDI Anaerobe (IF 2.3) Pub Date : 2023-11-04 Andrew M. Skinner, Xing Tan, Colin Westman, Thomas Birris, Emily N. Drwiega, Michael S. Wang, Stuart Johnson
Among 23 patients with multiply recurrent Clostridiodies difficile infection (mrCDI) who received bezlotoxumab at the end of antibiotic treatment a sustained clinical response of 91 % at 30 days and 78 % at 90 days was achieved. Bezlotoxumab administered at the end of antibiotic treatment was effective in patients with mrCDI.
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Description of Fusibacillus kribbianus gen. nov., sp. nov., a fusiform anaerobe isolated from pig feces Anaerobe (IF 2.3) Pub Date : 2023-11-02 Lu Bai, Jayoung Paek, Yeseul Shin, Hongik Kim, Si Hyun Kim, Jeong Hwan Shin, Joong-Ki Kook, Young-Hyo Chang
Objective The family Lachnospiraceae is affiliated with the order Clostridiales and was originally contained within Clostridial cluster XIVa. The members of Lachnospiraceae inhabiting the gut comprise the chemoorganotrophic genera, generating sundry short-chain fatty acids to supply energy to the host, and are considered to be related to obesity and gut health. Methods The polyphasic taxonomic approach
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Identification of root canal microbiota profiles of periapical tissue diseases using matrix-assisted laser desorption/ionization time-of-flight mass spectrometer Anaerobe (IF 2.3) Pub Date : 2023-11-02 Mete Ahlat, Cumhur Aydin, Sinem Kaya, Mehmet Baysallar
Objectives The purpose of this study was to identify microorganisms isolated from various periapical tissue diseases using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and classify them via an unsupervised machine learning approach. Methods A total of 150 patients with various apical conditions and teeth in need of endodontic retreatment were divided into
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Growth assessment of mixed cultures of probiotics and common pathogens Anaerobe (IF 2.3) Pub Date : 2023-10-30 Mansa Fredua-Agyeman, Paul Stapleton, Simon Gaisford
Objectives In this work, an isothermal microcalorimeter was applied to investigate the antipathogenic activity of three probiotics (Lactobacillus acidophilus, Bifidobacterium lactis and Bifidobacterium bifidum) against Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli using the probiotics in mixed culture with the pathogenic microorganisms. Methods A microcalorimeter was used to monitor
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Oral teicoplanin administration suppresses recurrence of Clostridioides difficile infection: Proof of concept Anaerobe (IF 2.3) Pub Date : 2023-10-23 Yoko Tanaka, Sho Tashiro, Shintaro Ikegami, Yuki Enoki, Kazuaki Taguchi, Kazuaki Matsumoto
Objectives Teicoplanin is a potential antimicrobial candidate for Clostridioides difficile infection (CDI) treatment. However, the therapeutic potential of teicoplanin against severe CDI has not been clinically proven. In the present study, we investigated the efficacy of oral teicoplanin administration against severe CDI and the recurrence of severe CDI after teicoplanin treatment in a mouse model
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Impact of nicotine and cotinine on macrophage inflammatory plasticity via vesicular modifications in gastrointestinal bacteria Anaerobe (IF 2.3) Pub Date : 2023-10-10 Jourdan E. Lakes, Xu Fu, Brock T Harvey, Khaga R. Neupane, Surya P. Aryal, Jessica L. Ferrell, Michael D. Flythe, Christopher I. Richards
Objectives This study aimed to elucidate mechanistic explanation(s) for compositional changes to enteric microflora by determining the impacts of continuous nicotine/cotinine exposure on representative gastrointestinal bacteria and how these alterations impact innate immune cell plasticity. Methods In vitro cultures of the gastrointestinal bacteria (Bacteroides fragilis 25285, Prevotella bryantii B14
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Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer Anaerobe (IF 2.3) Pub Date : 2023-10-06 Yuriko Matsumiya, Mitsukuni Suenaga, Toshiaki Ishikawa, Toshifumi Kudo, Tsuyoshi Nakagawa, Kentaro Okamoto, Masanori Tokunaga, Claudia Hurtado, Yuki Yamada, Kentaro Oka, Motomichi Takahashi, Luis Francisco Lopez Kostner, Miguel Luis O'Ryan Gallardo, Hiroyuki Uetake, Yusuke Kinugasa
Introduction Bacteroides fragilis (B. fragilis) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic B. fragilis (ETBF), a subtype of B. fragilis, produces an enterotoxin (BFT; B. fragilis toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of B. fragilis and ETBF on the clinical outcome of colorectal
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The impact of Akkermansia muciniphila and its extracellular vesicles in the regulation of serotonergic gene expression in a small intestine of mice Anaerobe (IF 2.3) Pub Date : 2023-10-04 Rezvan Yaghoubfar, Ehsan Zare BanadKoki, Fatemeh Ashrafian, Arefeh Shahryari, Arian Kariman, Mehdi Davari, Abolfazl Fateh, Shohreh Khatami, Seyed Davar Siadat
Objectives A better understanding of host-microbe interactions as a cross-talk between the gastrointestinal (GI) tract and the gut microbiota can help treat and prevent GI disorders by improving the maintenance of GI homeostasis. The gut microbiota can affect signaling molecules, such as serotonin, which regulates endocrine systems through the GI tract. Moreover, studying the effects of gut microbiota
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Metabolism of azo food dyes by bacterial members of the human gut microbiome Anaerobe (IF 2.3) Pub Date : 2023-09-27 Riley Elder, Sarah J. Vancuren, Alexander J. Botschner, P. David Josephy, Emma Allen-Vercoe
Objectives We set out to survey the capacities of bacterial isolates from the human gut microbiome to reduce common azo food dyes in vitro. Methods A total of 206 strains representative of 124 bacterial species and 6 phyla were screened in vitro using a simple azo dye decolorization assay. Strains which showed azoreductive activity were characterized by studies of azoreduction kinetics and bacterial
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Upregulation of the cfiA carbapenemase gene in a Bacteroides fragilis strain by the novel integrative and conjugative element Tn7563 Anaerobe (IF 2.3) Pub Date : 2023-09-23 Tore Taksdal Stubhaug, Nermin Zecic, Dagfinn Skaare
We describe Tn7563, a 31,844-bp integrative and conjugative element (ICE) carrying promoters upregulating the cfiA carbapenemase gene in Bacteroides fragilis strain Tbg-22. Excision and circularization of Tn7563 was demonstrated by PCR. Previously, only insertion sequences (IS) have been shown to carry mobile promoters for cfiA.
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Granular activated carbon enhances the anaerobic digestion of solid and liquid fractions of swine effluent at different mesophilic temperatures Anaerobe (IF 2.3) Pub Date : 2023-09-17 Miriam Orrantia, Edna R. Meza-Escalante, Vianey A. Burboa-Charis, Refugio B. García-Reyes, Marina M. Atilano-Camino, Denisse Serrano-Palacios, Luis A. Leyva, Yair A. Del Angel, Luis H. Alvarez
Objectives This study evaluated the effect of particle size and dosage of granular activated carbon (GAC) on methane production from the anaerobic digestion of raw effluent (RE) of swine wastewater, and the solid (SF) and liquid (LF) fractions. The effect of temperature using the selected size and dosage of GAC was also evaluated. Methods 60 mL of swine wastewater were inoculated with anaerobic granular
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Effect of formalin percentage, incubation time and temperature on Clostridium chauvoei culture inactivation and immunogenicity Anaerobe (IF 2.3) Pub Date : 2023-09-01 Niusha Adib, Azadeh Zahmatkesh, Parvaneh Esmaeilnejad-Ahranjani, Alireza Paradise, Lida Abdolmohammadi Khiav, Masoumeh Bagheri, Mohammad Abdoli, Atoosa Adib
Objectives In order to find the optimal inactivation conditions for Clostridium chauvoei culture, different factors were investigated and the immunogenicity of inactivated cultures was studied. Methods C. chauvoei was cultured with different formalin percentages (0.3, 0.5 or 0.7% V/V), inactivation temperatures (37 °C or room temperature) and incubation times (one or two weeks). Sterility tests were
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Pseudodesulfovibrio thermohalotolerans sp. nov., a novel obligately anaerobic, halotolerant, thermotolerant, and sulfate-reducing bacterium isolated from a western offshore hydrocarbon reservoir in India Anaerobe (IF 2.3) Pub Date : 2023-08-22 Saurabh L. Gaikwad, Soham D. Pore, Prashant K. Dhakephalkar, Sumit Singh Dagar, Rajeev Soni, Maninder Pal Kaur, Harshita Negi Rawat
Objective Characterization and documentation of strain MCM B-1480T, a novel sulfate-reducing bacterium isolated from produced water of India's western offshore hydrocarbon reservoir. Method Strain MCM B-1480T was unequivocally identified using a polyphasic approach routinely followed in bacterial systematics. The morphological and biochemical characterization of strain MCM B-1480T was carried out using
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Common laboratory tests and their correlation with the clinical presentation and prognosis of lemierre syndrome Anaerobe (IF 2.3) Pub Date : 2023-08-16 Riccardo M. Fumagalli, Elvira Gloor, Philippe A. Kaufmann, Maurus Frehner, Davide Voci, Stavros V. Konstantinides, Nils Kucher, Tommaso F. Nicoletti, Alessandro Pecci, Luca Valerio, Stefano Barco
Introduction Lemierre syndrome is a thromboembolic complication following an acute bacterial infection of the head/neck area, often due to anaerobes. Data on the prognostic role of laboratory parameters is lacking. Methods We analyzed individual-patient level data from a multinational cohort of patients with Lemierre-syndrome. Patients had an infection in the head/neck area, and contiguous vein thrombosis
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Prevalence of Clostridioides difficile in dogs (Canis familiaris) with gastrointestinal disorders in Rio de Janeiro Anaerobe (IF 2.3) Pub Date : 2023-08-12 Suzana Leite, Carlos Cotias, Kelly C. Rainha, Mayara Gil Santos, Bruno Penna, Renata F. F.Moraes, Céline Harmanus, Wiep Klaas Smits, Eliane de Oliveira Ferreira
Clostridioides difficile infections (CDI) have a high morbidity and mortality rate and have always been considered a nosocomial disease. Nonetheless, the number of cases of community-acquired CDI is increasing, and new evidence suggests additional C. difficile reservoirs exist. Pathogenic C. difficile strains have been found in livestock, domestic animals, and meat, so a zoonotic transmission has been
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Bacteremia due to Clostridium innocuum: Analysis of four cases and literature review Anaerobe (IF 2.3) Pub Date : 2023-08-09 Fernando Cobo, Virginia Pérez-Carrasco, María Tarriño-León, María Aguilera-Franco, José A. García-Salcedo, José María Navarro-Marí
Clostridium innocuum is a Gram-positive anaerobic spore-forming bacillus that has been identified as part of the normal intestinal microbiota. This bacterium has been rarely associated with human infections, and only few severe infections have been reported until now. In this work, we report on four patients with bacteremia due to C. innocuum, which were well identified by MALDI-TOF MS. Moreover, a
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The role of microbiome-based therapeutics for the reduction and prevention of antimicrobial-resistant organism colonization Anaerobe (IF 2.3) Pub Date : 2023-08-10 Armaghan-e-Rehman Mansoor, Caroline A. O'Neil, Jennie H. Kwon
The gut is host to a diverse array of microbiota that constitute a complex ecological system crucial to human physiology. Disruptors to the normal host microbiota, such as antimicrobials, can cause a loss of species diversity in the gut, reducing its ability to resist colonization by invading pathogens and potentially leading to colonization with antimicrobial resistant organisms (AROs). ARO negatively
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Probiotic-related Clostridium butyricum bacteremia: A case report and literature review Anaerobe (IF 2.3) Pub Date : 2023-08-05 Kazuhiro Ishikawa, Ryo Hasegawa, Koko Shibutani, Yumiko Mikami, Fujimi Kawai, Takahiro Matsuo, Yuki Uehara, Nobuyoshi Mori
We report three cases of Clostridium butyricum bacteremia associated with taking C. butyricum-related probiotics. We performed a literature review and found 11 cases of C. butyricum bacteremia including our cases. Nine cases related to probiotics. We should consider that probiotics may infect clinically unstable patients.
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Autolysin as a fibronectin receptor on the cell surface of Clostridium perfringens Anaerobe (IF 2.3) Pub Date : 2023-08-06 Riyo Aono, Shogo Emi, Kanako Okabe-Watanabe, Hirofumi Nariya, Nozomu Matsunaga, Yasuo Hitsumoto, Seiichi Katayama
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The outer membrane protein of Fusobacterium necrophorum, 43K OMP, stimulates inflammatory cytokine production through nuclear factor kappa B activation Anaerobe (IF 2.3) Pub Date : 2023-08-02 Xianjing He, Jiao Liu, Kai Jiang, Shuai Lian, Yu Shi, Shan Fu, Pengyu Zhao, Jiawei Xiao, Dongbo Sun, Donghua Guo
Objective Fusobacterium necrophorum causes bovine hepatic abscess, foot rot, mastitis, and endometritis. The 43 kDa outer membrane protein (43 K OMP) of F. necrophorum is a porin protein that plays an important role in infections by this bacterium, but the biological function and the pathogenesis of this protein are largely unknown. Methods In this study, we investigated the role of the 43 K OMP in
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The challenges of diagnosis and treatment of the rare Prevotella induced breast abscess: A retrospective cohort study Anaerobe (IF 2.3) Pub Date : 2023-07-25 I.M. Brouwer de Koning, S.W.T. Hoogmoet, N.H.M. Renders, Y.C.G.J. Paquay, M. Bessems, W.A. Draaisma, K. Bosscha
Objective Although the genus Prevotella is part of the general human flora, species of this anaerobic gram-negative bacteria have been described as cause of persisting nonpuerperal breast abscesses. Collecting punctate samples and testing these samples for anaerobic bacteria, is not part of the common diagnostic workflow in atypical breast abscesses. The causative anaerobic micro-organism can remain
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Ruminococcus gnavus bacteremia: Literature review and a case report associated to acute flare of ulcerative colitis in an immunocompromised patient Anaerobe (IF 2.3) Pub Date : 2023-07-21 Javier Martínez de Victoria Carazo, David Vinuesa García, Esther Serrano-Conde Sánchez, José Antonio Peregrina Rivas, Antonio José Ruíz Rodríguez, José Hernández Quero
We present a case of bacteremia caused by Ruminococcus gnavus in an immunocompromised patient. R. gnavus is a Gram-positive strict anaerobe bacterium that forms chains. The bacteremia has been associated with an acute flare of ulcerative colitis. Anaerobic bacteremia is becoming increasingly frequent in patients with compromised gastrointestinal barrier. The role of the human microbiota and its alterations
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The role of Actinomyces spp. And related organisms in cervicofacial infections: Pathomechanism, diagnosis and therapeutic aspects Anaerobe (IF 2.3) Pub Date : 2023-07-22 Zsanett Kövér, Vidar Johansen Nordskag, Ágnes Bán, Márió Gajdács, Edit Urbán
Members of the Actinomyces genus and Actinomyces-like organisms (ALOs; namely Actinotignum, Arcanobacterium, Schaalia and Varibaculum) are Gram-positive, non-spore-forming rods that are commensal members of the human oral cavity, gastrointestinal tract, female genital tract and skin flora. Cervicofacial actinomycosis or “lumpy jaw syndrome” – the chronic, suppurative granulomatous disease caused by
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Survival of bovine digital dermatitis treponemes in conditions relevant to the host and farm environment Anaerobe (IF 2.3) Pub Date : 2023-07-20 Jennifer Bell, Hayley E. Crosby-Durrani, Roger W. Blowey, Stuart D. Carter, Nicholas J. Evans
Objectives Bovine digital dermatitis (BDD), a painful infectious foot disease in dairy cattle, endemic in many countries worldwide, causes substantial economic and welfare impacts. Treponema spp. are considered key to BDD pathogenesis. To aid infection reservoir identification and control measure development, survival of BDD treponemes was investigated in different temperatures (4, 12, 20, 37, 45 and
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Biology activity and characterization of the functional L-HN fragment derivative of botulinum neurotoxin serotype E Anaerobe (IF 2.3) Pub Date : 2023-07-20 Xiao Tan, Cong-Cong Zhang, Jian-Sheng Lu, Zhi-Ying Li, Bo-Lin Li, Xu-Yang Liu, Yun-Zhou Yu, Qing Xu
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Membrane vesicles of Lactobacillus gasseri ATCC 19992 disrupt biofilms of vaginal pathogens Anaerobe (IF 2.3) Pub Date : 2023-07-17 Sameen Khan, Parul Shishpal, Vikrant M. Bhor
Membrane vesicles (MVs) are bioactive, nano-sized entities produced by all organisms. MVs of L. gasseri ATCC 19992 were isolated and their effect on the biofilms of vaginal pathogens, G. vaginalis and S. aureus was studied. The L. gasseri MVs resulted in significant disruption of biofilms of the vaginal pathogens.
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Antimicrobial susceptibility of Bacteroides fragilis group organisms in Hong Kong, 2020–2021 Anaerobe (IF 2.3) Pub Date : 2023-07-08 Hanshu Fang, Xin Li, Mei-Kum Yan, Man-Ki Tong, Kin-Hung Chow, Vincent Chi-Chung Cheng, Pak-Leung Ho
Objectives This retrospective study analyzed the susceptibility levels of Bacteroides fragilis group (BFG) in a hospital-based laboratory where disk diffusion test (DDT) was routinely performed. Isolates non-susceptible to imipenem and metronidazole by DDT were further investigated by gradient method. Methods The DDT and MIC susceptibility data of clindamycin, metronidazole, moxifloxacin and imipenem
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Type IV pili facilitated natural competence in Fusobacterium nucleatum Anaerobe (IF 2.3) Pub Date : 2023-07-13 Blake E. Sanders, Ariana Umaña, Tam T.D. Nguyen, Kevin J. Williams, Christopher C. Yoo, Michael A. Casasanta, Bryce Wozniak, Daniel J. Slade
Objectives Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first characterization of Type IV pili facilitated natural competence in Fusobacterium nucleatum, which is a Gram-negative, anaerobic bacterium that participates in a range
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Strain specificity in fusobacterial co-aggregation with colorectal cancer-relevant species Anaerobe (IF 2.3) Pub Date : 2023-07-08 Avery V. Robinson, Emma Allen-Vercoe
Objectives The purpose of the present study was to characterize co-aggregation interactions between isolates of Fusobacterium nucleatum subsp. animalis and other colorectal cancer (CRC)-relevant species. Methods Co-aggregation interactions were assessed by comparing optical density values following 2-h stationary strain co-incubations to strain optical density values when incubated alone. Co-aggregation
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Description of eight human infections caused by Bacteroides pyogenes in a tertiary hospital of northern Spain Anaerobe (IF 2.3) Pub Date : 2023-07-05 Domingo Fernández Vecilla, Mikel Joseba Urrutikoetxea Gutiérrez, Mary Paz Roche Matheus, Itziar Angulo López, Cristina Aspichueta Vivanco, Felicitas Elena Calvo Muro, José Luis Díaz de Tuesta del Arco
Bacteroides pyogenes is a Gram-negative obligate anaerobe, saccharolytic, non-motile, non-pigment-producing and non-spore-forming rod. Reports of human infections caused by B. pyogenes are scarce, with approximately 30 cases documented in scientific literature. The aim of this study was to describe the clinical characteristics of 8 different patients and to study the in vitro antibiotic susceptibility